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Bacillus for preventing and treating root rot of paris polyphylla, and preparation method and application thereof

A technique of Aesculus aesculus and Bacillus, applied in the field of biocontrol bacteria, can solve problems such as economic loss, excessive residue of carbendazim, and obstacles to repeated cropping, and achieve the effects of reducing economic loss, avoiding residual pollution, and reducing harm

Active Publication Date: 2020-02-18
汉江师范学院
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AI Technical Summary

Problems solved by technology

[0003] Root rot is one of the most important diseases of Aescinus aesculus. It is a soil-borne disease and spreads through the soil. It is especially serious in wet and rainy seasons, and it is easy to cause large-scale epidemics and cause Aescinus aescini to cease production. Seriously affect the yield, limit the development of the Chinese medicinal material industry, and cause major economic losses; currently, it is found that Fusarium is the main pathogen causing root rot of Aesculus aesculi. Studies have shown that 5 species of Fusarium (F. .graminearumf.sp.paris, F.oxysporum, F.equiseti, F.tricinctum) can infect the rhizome of Aesculus aesculus and cause root rot;
[0004] At present, the prevention and control measures of root rot are mainly spraying carbendazim or chemical agents to disinfect the soil, but the effect is not ideal. Many years of prevention and control have led to the increase of pathogenic bacteria resistance, soil degradation, repeated cropping obstacles, excessive carbendazim residues and many other problems. Seriously, the yield and quality of Aesculus aescini are affected; therefore, it is of great significance to discover strains with biocontrol potential and develop biogenic fungicides that can replace chemical fungicides for the prevention and treatment of Aescinus aesculus root rot

Method used

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  • Bacillus for preventing and treating root rot of paris polyphylla, and preparation method and application thereof
  • Bacillus for preventing and treating root rot of paris polyphylla, and preparation method and application thereof
  • Bacillus for preventing and treating root rot of paris polyphylla, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, the specific preparation steps of the Paenibacillus polymyxa strain HJ-2 are as follows:

[0045] Step 1: Isolation of Paenibacillus polymyxa strain HJ-2

[0046] (1) Soil sample collection: healthy Aesculus aesculus rhizosphere soil at a depth of 8-12cm was collected from the planting base of Aesculus aesculata in Shiyan City, Hubei Province, and brought back to the laboratory;

[0047] (2) Put 1 g of rhizosphere soil into a 250 ml sterilized Erlenmeyer flask, add 100 ml of sterile water, 30-40 sterilized glass beads, shake at 160 rpm on a shaker for 30 min, and let stand for 30 min;

[0048] (3) Take the supernatant and dilute the concentration to 10 by gradient dilution method. -4 、10 -5 , 10- 6 For three gradients, draw 0.1ml of liquid from the three gradients and inoculate them on beef extract peptone solid medium, and incubate at a constant temperature of 30°C for 48 hours;

[0049] (4) Pick a single colony with different culture characteristics,...

Embodiment 2

[0056] Embodiment two, test the bacteriostatic rate of Paenibacillus polymyxa HJ-2 to different Fusarium

[0057] (1) Connect F.solani f.sp. paris, F.graminearum f.sp.paris, F.oxysporum, F. equiseti, F.tricinctum bacteria cakes in the center of the PDA solid medium plate, at 3.0cm on one side of the bacteria cakes Place a sterile filter paper round cake with a diameter of 6mm, connect the sterile filter paper to the Paenibacillus polymyxa strain HJ-2, add blank control on the other side, connect the sterile filter paper to sterile water, repeat 5 times for each test strain, and set Cultivate in the dark in a 25°C incubator;

[0058] (2) observe and measure the bacteriostatic zone width after 4-8 days according to pathogen growth rate, measure bacteriostasis rate, concrete result is as shown in table 2;

[0059] Table 2: Inhibitory rate of Paenibacillus polymyxa HJ-2 to different Fusarium

[0060]

[0061] Bacterial inhibition rate=(control side colony radius-contrast side...

Embodiment 3

[0063] Embodiment three, detecting the influence of Paenibacillus polymyxa strain HJ-2 fermentation filtrate on the zoospore activity of Fusarium F. oxysporum, concrete steps are as follows:

[0064] Step 1: After activating the strain HJ-2, inoculate it into a 250mL Erlenmeyer flask containing 50mL of fermentation medium, shake and culture it on a shaker at 28°C with a rotation speed of 150rpm for 2 days, so that the concentration of the bacterial solution reaches 2.0×10 9 cfu / ml, after the fermentation broth was centrifuged at 12000rpm for 5min, it was filtered with a 0.22 μm bacterial filter to obtain a sterile fermentation filtrate;

[0065] Step 2: Divide into three groups of experiments, one group takes 450 μl zoospores + 450 μl sterile fermentation filtrate, the other group takes 450 μl zoospores + 450 μl fermentation filtrate, and uses 450 μl zoospores + 450 μl sterile water suspension as a control, Placed at 25°C, microscopic observation was carried out every 3 hours,...

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Abstract

The invention discloses bacillus for preventing and treating root rot of paris polyphylla, and a preparation method and application thereof. A paenibacillus polymyxa strain HJ-2 is adopted to infect the fusarium zoospores, then germination of fusarium spore is further inhibited, and harm of fusarium to rhizome parts of the paris polyphylla is reduced, so that harm of root rot to the paris polyphylla is reduced, the yield of the paris polyphylla is ensured, and economic loss of pesticide farmers is reduced. In addition, the paenibacillus polymyxa strain HJ-2 is used for biological prevention, so that pesticide residues are not generated, and the bacillus provided by the invention has the characteristics of greenness and recyclability, good prevention and control effect, avoidance of pesticide residue pollution and the like.

Description

technical field [0001] The invention relates to the technical field of biocontrol bacteria, in particular to a bacillus for preventing and treating Aescinus root rot and its preparation method and application. Background technique [0002] Aescin is a plant of the genus Paris in the family Liliaceae. It mostly grows in the shade of forests at an altitude of 800-1300 meters. It is distributed in Fangxian, Zhushan, Zhuxi, Wudang Mountain, Fuzhou, Shiyan City. Longshan (Saiwudang) is the most distinctive authentic medicinal material in Northwest Hubei, "Four Ones". Yizhihua is an important prescription drug for many famous and precious Chinese patent medicines such as Yunnan Baiyao, Jidesheng Sheyao, Gongxuening; due to the generally high altitude and high forest coverage in Shiyan, the planting area of ​​Aescin and surrounding counties and cities The largest, Aesculus aesculus in Fulong Mountain, Shiyan City has been listed as a geographical indication product by the country;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P3/00C12R1/12
CPCA01N63/00C12N1/20C12N1/205C12R2001/12
Inventor 柯尊伟江爱明巩建华龙德清陈立孙良学
Owner 汉江师范学院
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