Method for generating sporangia and releasing zoospore by inducing phytophthora capsici

A technology for Phytophthora capsicum and zoospores, which is applied in the field of microbial culture, can solve the problems of easy pollution, difficult to obtain plant materials, and complicated methods, and achieves the effect of shortening the induction period.

Active Publication Date: 2016-03-30
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It takes more than 10 days for this method to induce Phytophthora to produce sporangia, which is a long time; meanwhile, the soil leachate is not strictly sterilized, and the skin culture medium is sterilized by moist heat, so the effect of inducing zoosporangia is unstable and easy to contaminate
There are other induction methods that can also produce a large number of zoospores: rye culture medium to culture mycelium overgrown the plate, smear the medium plate covered with mycelia with a sterile L-shaped glass rod, and place it at 28°C for light biochemical Cultivate in an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Phytophthora capsici standard sequencing strain produces sporangia and zoospores

[0027] (1) Inoculate Phytophthora capsici (strain number: LT1534, standard strain for sequencing) on ​​the carrot medium on a clean bench, and incubate in the dark at 25°C for 2 days.

[0028] (2) On the ultra-clean workbench, cut 0.3 × 0.3cm mycelium pieces with a scalpel blade at the edge of the colony, get 20 pieces of mycelium pieces and place them in a sterile petri dish with a diameter of 11cm, and add a volume concentration of 30 mL of 15% V8 vegetable juice, cultured in the dark at 25°C for 2 days; then pour out the V8 vegetable juice, wash it once with sterilized deionized water, and replace it with sterile 0.3g / LCaCO 3 15mL, placed in a light incubator with a white fluorescent lamp with a light intensity of 800Lux, and cultured in continuous light at 25°C for 2 days.

[0029] (3) Place the Phytophthora capsici that has undergone the above induced culture in a refriger...

Embodiment 2

[0031] Example 2 Induces Phytophthora capsici strains isolated in fresh pepper blight samples to produce sporangia and zoospores

[0032] (1) Take freshly-onset capsicum blight specimens, and use scissors to cut out tissue blocks with a size of about 5mm×5mm at the junction of disease and health.

[0033] (2) On the ultra-clean workbench, soak the tissue block in 75% alcohol for 30s, then soak and rinse in 5% sodium hypochlorite solution for 1min; then wash it with sterilized deionized water for 3 times. After the water was blotted dry on sterile filter paper, it was transferred to a carrot medium plate and incubated in the dark at 25°C for 3 days.

[0034] (3) After the colony grows, pick the border mycelium block whose colony color is white on the ultra-clean workbench, and purify it to obtain Phytophthora capsici.

[0035] (4) On the ultra-clean workbench, use a scalpel blade to cut 1cm×1cm mycelium blocks at the edge of the colony, take 20 mycelium blocks and place them i...

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PUM

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Abstract

The invention discloses a method for generating sporangia and releasing zoospore by inducing phytophthora capsici. The method includes the steps that phytophthora capsici is activated, an inducing solution for generating sporangia is prepared, sporangia are induced to be generated, and zoospore is induced to be released. The inducing solution used in the method is originally imported V8 vegetable juice with the volume concentration being 10-15% and a CaCO3 solution with the volume concentration being 0.2-0.3 g/L. When phytophthora capsici is induced to generate the sporangia, continuous light culture is carried out under a white fluorescent lamp. The method is simple, convenient to use, quick, free of pollution and capable of being used in phytophthora capsici pathogenicity measurement and biological activity measurement, achieved through an antibacterial agent, of phytophthora capsici.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a method for inducing Phytophthora capsici to produce sporangia and release zoospores. Background technique [0002] Pepper blight is a devastating fungal disease that occurs worldwide. It was first discovered in New Mexico, USA in 1918. Phytophthora capsici Leonian, which caused pepper blight, was named by Leon Leonian in 1922. It belongs to plant pathogenic oomycetes. Phytophthora capsici overwinters in diseased soil as oospores or chlamydospores, and can survive for several months or even longer. It has a wide range of hosts and mainly infects many important vegetable crops such as peppers, tomatoes, eggplants, cucumbers, and pumpkins. Capsicum blight can occur in both open fields and protected areas. The prevalence of the disease has caused serious losses of peppers. The general rate of diseased plants is 15-30%, and it can reach more than 80% in sev...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
Inventor 刘裴清陈庆河李本金翁启勇
Owner INST OF PLANT PROTECTION FAAS
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