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Method of preparing microbial feed additive for reducing cholesterol level of vitellus

A microbial feed and cholesterol technology, applied in animal feed, animal feed, application, etc., can solve the problems of poor enzymatic hydrolysis effect and low utilization of soybean protein, and achieve the effects of short mutagenesis cycle, high-speed growth, and low site requirements

Inactive Publication Date: 2008-02-27
SHANGHAI CHUANGBO ECOLOGICAL ENG
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AI Technical Summary

Problems solved by technology

[0006] The use of Bacillus subtilis to ferment soybeans has been used earlier in Japan. Natto, a nutritional and health food that is all the rage in Japan, is fermented by Bacillus subtilis. Poor, low utilization of soybean protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1: Breeding of Fermentation Strains

[0035]a. Strain subculture: Bacillus licheniformis (ACCC10619) strain provided by the Culture Conservation Center of the Ministry of Agriculture was used for subculture on nutrient agar medium. Streptococcus lactis (ACCC10653) was subcultured through lactic acid bacteria medium, and the strains with fast growth and obvious colony characteristics were selected and preserved at 4°C;

[0036] b. Compound induction of strains: the preserved Bacillus subtilis was diluted to 10 with 0.9% normal saline -8 , treat the bacterial suspension with low heat in a microwave oven for 42S, count by hemocytometer, spread the treated bacterial suspension on a starch medium plate, and incubate it upside down at 37°C for 48 hours, and the culture medium is transparent Bacterial colonies with a ratio of ring diameter to colony diameter (HC value) greater than 1.0 are inoculated into nutrient agar test tubes for preservation;

[0037] Make bacteria...

Embodiment 2

[0049] Step 1: Breeding of Fermentation Strains

[0050] a. strain passage: with embodiment 1;

[0051] b. Compound induction of bacterial strains: Dilute the preserved Bacillus subtilis to 10-9 with 0.9% normal saline, treat the bacterial suspension in a microwave oven for 55 seconds with low heat, count by hemocytometer, and the treated bacteria The suspension was spread on a flat plate of starch medium, and cultured upside down at 35°C for 48 hours, and colonies with a ratio of transparent circle diameter to colony diameter (HC value) greater than 0.8 in the medium were selected and inoculated into nutrient agar test tubes Save in;

[0052] Make bacterial suspensions of Bifidobacterium, Lactobacillus plantarum and Streptococcus lactis respectively, and make spore suspensions of Bacillus subtilis, dilute to 10 -7 , at 30cm, use a UV lamp with a power of 20W and a wavelength of 260nm for 30 seconds. The treated bacterial suspension is cultured upside down on a lactic acid b...

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Abstract

The invention discloses a microbe additive and making method by using cholesterol metabolic path to modulate egg poultry through lactic acid bacteria, which is characterized by the following: utilizing bacillus subtilis to manufacture soybean polypeptide; reducing the content of yolk cholesterol; combining the microwave induction and ray induction to ferment bacteria; improving the sieving production and enzyme production; accelerating the growth of soybean polypeptide; improving the egg production and immunity of egg poultry; reducing the usage of antibiotics; providing a safe and high-effective agricultural biological agent.

Description

technical field [0001] A microbial feed additive and a preparation method that utilizes lactic acid bacteria and Bacillus subtilis to regulate the cholesterol metabolism pathway of laying poultry, thereby reducing the cholesterol content of egg yolk in poultry egg production, can effectively reduce the cholesterol content of poultry egg yolk, and at the same time reduce antibiotic residues in poultry eggs content, belonging to the technical field of agricultural biological products. Background technique [0002] my country is the world's largest producer and consumer of poultry eggs, but the quality of poultry eggs is relatively low, mainly due to the high-density feeding in poultry breeding, which adds a large amount of antibiotics to prevent diseases of poultry and poultry, resulting in antibiotics, veterinary drugs , Feed additive residues seriously exceed the standard; at the same time, the animal source protein added in the feed makes the cholesterol content in the egg y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K10/18
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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