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Polypeptide preparation for microorganism ferment making feed and preparation method

A microbial fermentation and feed technology, which is applied in animal feed, animal feed, microbial/single-cell algae protein processing, etc., can solve problems such as limited application range and potential safety hazards, and achieve guaranteed survival time, low site requirements, and improved daily The effect of feed intake

Inactive Publication Date: 2008-02-13
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its production process only uses physical methods to extract peptides from animal raw materials, which has certain potential safety hazards. At the same time, it uses pharmaceutical raw materials as components, and its application range is not wide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Step 1: Breeding of Fermentation Strains

[0043] a. Subculture of strains: Bacillus subtilis strains, Lactobacillus plantarum and Lactobacillus casei provided by the Culture Preservation Center of the Ministry of Agriculture are used to subculture through nutrient agar medium, and line separation is drawn during the cultivation process to select for fast growth and colony characteristics For obvious strains, the strains should be preserved at 4°C;

[0044] b. Composite induction of strains: the Bacillus subtilis preserved in step a is diluted to 10 with 0.9% normal saline -5 , treat the bacterial suspension with a low fire for 30 seconds in a microwave oven, count by hemocytometer, compare the number of viable bacteria before and after treatment, spread the processed bacterial suspension with a higher survival rate on a flat plate of starch medium, Inverted culture at 35°C for 48 hours, select colonies with a ratio of transparent circle diameter to colony diameter gre...

Embodiment 2

[0062] Step 1: Breeding of Fermentation Strains

[0063] a. Bacterial strain passage: Same as Example 1.

[0064] b. Compound induction of strains: the preserved Bacillus subtilis was diluted to 10 with 0.9% normal saline -7 , treat the bacterial suspension with low heat in a microwave oven for 45S, count by hemocytometer, spread the treated bacterial suspension on a starch medium plate, and culture it upside down at 37°C for 48 hours, and select the culture medium The colony with the larger ratio of the diameter of the middle transparent circle to the diameter of the colony (HC value) was inoculated into a nutrient agar test tube for preservation. Dilute the preserved strain to 10 -7 . At 30cm place, use a power of 20W UV lamp (wavelength is 260nm) to treat for 25s, and the bacteria suspension after treatment is cultivated upside down at 35°C for 48h on the nutrient agar plate, and the colony with fast growth rate is selected for shake flask experiment. Observe the growth...

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Abstract

The present invention relates to a preparation method for microbial polypeptide preparation with the use of lactic acid bacteria and bacillus subtilis, and which is characterized in that the high yield enzyme and high quantity production strains is selected and trough the integration of original microwave-induction and rays-induction, wherein the production process applies the liquid-alone culture and solid-composite culture, adds special product promoter into the medium, the content of soybean peptide is high, at the same time which is rich in metabolic products such as peptidoglycan, microbial antioxidants and enzymes. The present invention has the advantage of low production cost, faster cycle, and faster absorption for animal after breeding, high daily increasing in weight, immunity enhancing, and stress rising, which has obvious effect in breeding and is a safe and high efficient new feed additive.

Description

technical field [0001] The invention relates to a polypeptide preparation and preparation method for microbial fermentation to produce feed, in particular to a polypeptide preparation and preparation method for producing feed using Bacillus subtilis and two lactic acid bacteria. Adding it to animals can improve the immunity of animals. The invention improves the digestion and absorption function of farmed animals and improves the stress of animals, and belongs to the technical field of agricultural biological products. Background technique [0002] Protein is the basic substance of the structure and function of animal cells. At present, there are two kinds of protein in our feed, soybean protein and animal protein, which are mainly used by animals in the form of amino acids on the wall of the small intestine after the action of digestive juice in the digestive tract of animals. Modern biological metabolism experiments have proved that the intestinal absorption of short pepti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23J3/34A23J3/20A23K10/12A23K10/37
CPCY02P60/87
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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