Tissue culture and rapid propagation method of highly efficient induction of cluster buds of davidia involucrata

Abstract

The invention discloses a tissue culture and rapid propagation method of highly efficient induction of cluster buds of davidia involucrata. The method includes the specific steps: 1) selecting davidia involucrata branches as mother plants, wherein the davidia involucrata branches have robust growth, are free of pest and disease damage, have good characters and form lateral buds without leaf expansion, and soaking the davidia involucrata branches in a GA3 solution until the lateral buds of the davidia involucrata grow to 3 cm or above; 2) shearing the lateral buds, conducting disinfection, inoculating the lateral buds to a culture medium which contains Wpm, 1.2 mg / L<-1> of KT, 0.3 mg / L<-1> of NAA, 0.5 mg / L<-1> of 6-BA, 3.5 g / L<-1> of agar, 20 g / L<-1> of sucrose and 0.2 g / L<-1> of AC, and conducting cultivation for 18-22 days until bud points germinate and grow to form the cluster buds. By the use of a provided davidia involucrata cluster bud induction technology, the method is simple, the induction cycle is short, the propagation coefficient is high, and the method has a certain role in promoting the national level protection of rare and endangered plants.

Description

technical field [0001] The invention relates to a plant tissue culture propagation technology, in particular to a tissue culture rapid propagation method for efficiently inducing clustered buds of Davidia involucrata. Background technique [0002] Dove tree is a deciduous tree. It can grow to a height of 15-25 meters, and the leaves are broadly ovate with serrated edges. The beautiful color flowers are relict plants left by the Cenozoic Tertiary period 10 million years ago. During the Quaternary glacial period, most of the dove tree became extinct one after another, and only survived in some areas in southern China, becoming a plant kingdom. Today's "living fossil" is known as "China's pigeon tree", also known as "pigeon flower tree" and "water pear". The wild species only grow in the surrounding areas of Sichuan Province and Hubei Province in China. Davidia involucrata has been listed as a first-class national key protected wild plant. It is a unique single genus plant in...

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Problems solved by technology

[0003] At present, the propagation technology of Davidia involucrata is mainly based on sowing and raising seedlings, but this method has the following disadvantages: the seedling formation period is long, and the emergence time is 3-10 years; The traits have certain influence; at the same time, a large amount of manpower, material resources and land resources are required, and the production cost is high; in summary, it is necessary to find a fast seedling emergence and low production cost method of tissue culture and rapid propagation of Davidia involucrata to promote industrial production

[0004] "Tissue Culture and Plant Regeneration of the Endangered Plant Doveia involucrata" published by Zou Lijuan of Southwest Normal University in "Plant Research" discloses a rapid propagation method using the lateral buds of Doveia involucrata to directly induce clustered buds, but this method has the limitation of explant collection time. The disadvantages of limited and long induction time, and its value-added rate and multiplication multiple have a certain room for improvement; Xia Han of Sichuan Agricultural University published "Research on the First Generation of Davidia involucrata" and Luo Shirong of Hubei University for Nationalities published "Tissue Culture of Davidia involucrata Buds" It is mentioned in "Research" that the lateral buds of Davidia involucrata are used as explants to induce buds, but they can only promote the germination of single buds, and cannot induce clustered buds.

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