3D full-layer skin model, culture medium for forming 3D full-layer skin model and preparation method of 3D full-layer skin model

Abstract

The invention discloses a 3D full-layer skin model, a culture medium for forming the 3D full-layer skin model and a preparation method of the 3D full-layer skin model. The culture medium comprises a protein preparation, and the protein preparation comprises the following components in percentage by mass: 0.1-99% of albumin, 0.1-99% of globulin and 0.1-99% of fibronectin. Serum is not needed in thegas-liquid phase process of preparing the 3D full-thickness skin model by combining the culture medium with pig type I collagen; the prepared 3D full-layer skin model is complete, the activity and the stability are remarkably improved, the application field of the type of model is expanded, and large-scale industrial production of the skin model is achieved.

Description

technical field [0001] The invention relates to the field of skin tissue engineering. In particular, it relates to a 3D full-thickness skin model, a culture medium for its formation, and a preparation method. Background technique [0002] The skin is the largest organ in the human body. It acts as an important barrier between the external environment and the internal organs, protecting the body from water loss, environmental influences, injury or infection, and helping to regulate body temperature. Due to its exposure to the outside world, it is susceptible to damage such as mechanical, thermal or chemical damage. Although the skin barrier is usually self-repairing, various factors such as infection, oxidation or large wound area can make the skin barrier difficult to repair. Skin inoculation is usually chosen in clinical treatment, but it is limited by the available sites of healthy donors, such as the limited area of ​​available sites, so many skin substitutes have been d...

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the disadvantages of poor controllability of the skin model provided by the current prior art, poor stability of the construction method, high cost, complex synthesis technology that is not conducive to industrial production, and fast collagen degradation during the construction process. , providing a 3D full-thickness skin model and a culture medium and preparation method for its formation

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