Method for inducing phytophthora parasitica var. nicotianae to generate zoosporangium and release spore

A technology of Phytophthora nicotianae and Phytophthora nicotianae is applied in the field of microbial culture, which can solve the problems such as the release rate of zoospores needs to be improved, the effect of zoosporangium is unstable, and the induction time is long, so as to improve the induction effect, efficiency and activity. The effect of strong ability and shortened time

Active Publication Date: 2011-10-26
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Abstract
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Problems solved by technology

It can also be taken: after Phytophthora nicotianae grows mycelium on oat medium, drop the Peeter's culture solution of soil leaching liquid to induce zoosporangia, after low temperature treatment, then cultivate at an appropriate temperature to produce a large number of zoospores [Yang Jianqing , Jiang Tong, Chen Xueping. Research on the cultivation of Phytophthora nicotiana and the method of mass production of zoosporangia and zoospores. Plant Protection, 2001, 27(4):12-14.], but because the soil leachate was not strictly sterilized Bacteria and Petri culture fluid are sterilized by moist heat, the effect of inducing zoosporangia is unstable and easy to contaminate
Chinese invention patent ZL...

Method used

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Effect test

Embodiment 1

[0030] The method for inducing Phytophthora tabacum to produce zoosporangia and release spores takes the following steps:

[0031] A. Mycelia culture of Phytophthora nicotiana: Take the activated Phytophthora nicotiana mycelium block, inoculate it on a 10mL oat medium plate made by a 100 mL narrow mouth Erlenmeyer flask, and cultivate it at 26-28°C for 10 days;

[0032] B. Prepare the induction solution for producing zoosporangia: select tobacco seedlings with 6-7 true leaves of the tobacco variety susceptible to black shank (Safflower Dajinyuan) fully expanded, take out the root system of the tobacco seedlings completely, and rinse them with tap water to remove them The soil or seedling substrate on the root system is shaken or blotted dry; the root water mixture is made by adding 20ml of tap water to 1g of fresh root system, smashed or ground into a suspension that can pass through a 40-mesh fluid sieve, and use 5 times the volume Wash the screen with tap water, dilute the f...

Embodiment 2

[0037] The method for inducing Phytophthora tabacum to produce zoosporangia and release spores takes the following steps:

[0038] A. Mycelia culture of Phytophthora nicotiana: Take the activated Phytophthora nicotiana mycelium block, inoculate it on a 15mL oat medium plate in a 150 mL narrow-mouth Erlenmeyer flask, and cultivate it at 26-28°C for 12 days;

[0039]B. Preparation of induction solution for producing zoosporangia: select tobacco seedlings with 6 to 7 true leaves of the tobacco variety susceptible to black shank (KRK26) fully expanded, take out the root system of the tobacco seedlings completely, rinse with tap water to remove Soil or seedling substrate, shake dry or absorb clean water; make root water mixture by adding 20ml of tap water to 2g of fresh roots, mash or grind it into a suspension that can pass through a 40-mesh fluid sieve, and rinse with 5 times the volume of tap water Sieve, dilute the filtrate, and mix well; after suction filtration with filter pa...

Embodiment 3

[0044] The method for inducing Phytophthora nicotianae to produce zoosporangia and release spores takes the following steps:

[0045] A. Mycelia cultivation of Phytophthora nicotiana: Take the activated Phytophthora nicotiana mycelium block, inoculate it on a 15mL oat medium plate in a 100mL narrow-mouth Erlenmeyer flask, and cultivate it at 26-28°C for 11 days;

[0046] B. Preparation of induction solution for producing zoosporangia: select tobacco seedlings with 6 to 7 true leaves of the tobacco variety susceptible to black shank (Xiaojin 1025) fully unfolded, take out the root system of the tobacco seedling completely, and rinse with tap water to remove the root system The soil or seedling substrate on the ground, shake dry or absorb open water; make a root water mixture according to the ratio of 20ml tap water and 1.5g fresh root system, smash or grind it into a suspension that can pass through a 40-mesh fluid sieve, and use 5 times the volume Wash the sieve with tap water...

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Abstract

The invention discloses a method for inducing phytophthora parasitica var. nicotianae to generate zoosporangia and release spores. The method comprises steps of: culturing phytophthora parasitica var. nicotianae mycelia on an oat medium prepared in a narrow mouth conical flask; preparing an induction liquid for generation of zoosporangia by selecting roots of tobacco seedlings, with 6-7 pieces of true leaves completely expanding, of a black shank infected variety; inducing the mycelia with the induction liquid to generate zoosporangia and to release spores. According to the invention, a characteristic that a black shank infected tobacco variety is more easily to generate affinity reaction with phytophthora parasitica var. nicotianae is employed, and a filtrate of seedling roots of the black shank infected tobacco variety is used as the induction liquid, so as to induce the cultured mycelia to generate zoosporangia. Proved by tests, the invention has a short induction time, high zoospore release rate, high obtained zoospore content; and scraping mycelia for culture is not needed. Therefore, the invention is of less pollution and easy to operate.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a method for inducing Phytophthora nicotianae to produce zoosporangia and release spores with good effect, high efficiency and low pollution rate. Background technique [0002] Phytophthora tobacco ( Phytophthora parasitica var . nicotianae ) is the pathogen of tobacco black shank. Under natural conditions, zoospores are mainly used to infect tobacco plants from the roots or stem bases. In artificial culture, it can be cultured by culturing hyphae, inducing zoosporangia, and low-temperature treatment. The zoosporangium releases zoospores, thereby obtaining a greater number of zoospores. Commonly used induction methods are: using oat plate to culture mycelium, 0.1% KNO 3 Solution infiltration can induce Phytophthora nicotianae to produce zoosporangia[Gooding G V, Lucas G B. Factors influencing sporangail formation and zoospore activity in Phytoph...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
Inventor 方敦煌晋艳王成宋春满秦西云
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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