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Method for evaluating pepper phytophthora blight resistance of crops

A technology for pepper blight and crops, which is applied to biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve the problems of large space occupation and long time, and achieve the effects of small space occupation, labor cost saving, and experimental period shortening.

Inactive Publication Date: 2017-05-10
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used mycelium block wound method needs to sow and cultivate the pepper varieties to be tested until they become seedlings, and then transplant.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Methods for Evaluating Capsicum Varieties Resistance to Capsicum Blight

[0013] (1) Preparation of pepper seedlings: The seeds of American Xiyuan pepper (susceptible variety) and Minjiao No. 3 (disease-resistant variety) were surface-sterilized with 30% sodium hypochlorite, rinsed with sterilized distilled water, and blanched in a water bath at 56°C. After 20 minutes of planting, the seeds were sown on 4 layers of wet filter paper, cultivated at a relative humidity of 85%, light intensity of 4000 LUX, and a temperature of 25°C, and 4-leaf seedlings were formed after 8 days for future use;

[0014] (2) Preparation of zoospore suspension of Phytophthora capsici: Inoculate Phytophthora capsici model fungus LT1534 on a 10% V8 medium plate, culture in the dark at 25°C for 8 days until the mycelia cover the entire plate, open the sealing film Irradiate for 3 days at a light intensity of 7000 LUX, add sterile water to cover the entire plate overnight, treat at 4°C for 17 minu...

Embodiment 2

[0019] Method for evaluating resistance of watermelon varieties to capsicum blight

[0020] (1) Preparation of watermelon seedlings: Xinhongbao (susceptible variety) and Xiangximelon No. 6 seeds (disease-resistant variety) were surface-sterilized with 30% sodium hypochlorite, rinsed with sterilized distilled water, and blanched in a water bath at 56°C. After 20 minutes of planting, the seeds were sown on 4 layers of wet filter paper, cultivated at a relative humidity of 85%, light intensity of 4000 LUX, and a temperature of 25°C, and 4-leaf seedlings were formed after 8 days for future use;

[0021] (2) Preparation of zoospore suspension of Phytophthora capsici LT1534: Inoculate Phytophthora capsici model fungus LT1534 on a 10% V8 medium plate, culture in the dark at 25°C for 7-10 days until the mycelia cover the entire plate, and open the seal The membrane was irradiated for 3-4 days under the light intensity of 7000 LUX, and the whole plate was covered with sterile water ove...

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PUM

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Abstract

The invention discloses a method for evaluating the pepper phytophthora blight resistance of a crop. The method comprises the specific steps of preparation of seedlings, preparation of phytophthora capsici zoospore suspension liquid, root-irrigation and inoculation, statistics of morbidity and evaluation of a disease-resistant result. Quantitative phytophthora capsici zoospores are inoculated into the seedling root parts of the crop, it is avoided that the same variety anti-infection shows difference due to excessive inoculation amount, and during resistance identification, the method is more accurate and effective; in the actual operation, inoculation can be conducted in a large batch mode, and the method has the advantages of being small in occupied space, saving labor cost and shortening test period. Accordingly, the method is suitable for application and popularization.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, and in particular relates to a method for evaluating crop resistance to capsicum blight. Background technique [0002] Pepper blight is a devastating fungal disease that occurs worldwide. It was first discovered in New Mexico, USA in 1918. Phytophthora capsici Leonian, which caused pepper blight, was named by Leon Leonian in 1922. It belongs to plant pathogenic eggs bacteria. Phytophthora capsici overwinters in diseased soil as oospores or chlamydospores, and can survive for several months or even longer. It has a wide range of hosts and mainly infects many important vegetable crops such as peppers, tomatoes, eggplants, cucumbers, and pumpkins. Capsicum blight can occur in both open fields and protected areas. The prevalence of the disease has caused serious losses of peppers. The general rate of diseased plants is 15-30%, and it can reach more than 80% in severe ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 刘裴清李本金丁雪玲翁启勇陈庆河
Owner INST OF PLANT PROTECTION FAAS
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