Method for separating monosporangiums of potato late blight pathogens

A technology of potato late blight and monosporangium, applied in the field of microorganisms, can solve the problems of low success rate, time-consuming, difficult operation, etc., and achieve the effect of reducing time and improving operation efficiency

Inactive Publication Date: 2016-04-13
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for isolating monosporangia of potato infestans, which s...

Method used

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  • Method for separating monosporangiums of potato late blight pathogens
  • Method for separating monosporangiums of potato late blight pathogens
  • Method for separating monosporangiums of potato late blight pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Pick a little mycelium from the diseased potato leaves with an inoculation needle, stir it several times in a 2ml centrifuge tube containing 200ul sterile water, and mix the mycelia and sporangia on the inoculation needle with sterile water, then use Vortex with a rotating speed of 1500rpm / min for 30s, repeat twice; then adjust the concentration of the sporangia suspension with a hemocytometer to adjust the concentration of the sporangia suspension to 1 / 2ul;

[0021] (2) Place three coverslips that have been sterilized with 75% ethanol on a glass slide, drop a drop of sporangia suspension on the three coverslips respectively, and then inspect under a 10×10 times microscope. To a single sporangia, add a 0.8cm×0.8cm rye culture medium on the droplet, then transfer it to a sterilized empty petri dish, and seal it;

[0022] (3) Put the petri dish in a refrigerator at 4°C for 3 hours at low temperature to stimulate the sporangia to release zoospores, then transfer to an ...

Embodiment 2

[0025] (1) Pick a little mycelium from the diseased potato leaves with an inoculation needle, stir it several times in a 2ml centrifuge tube containing 200ul sterile water, and mix the mycelia and sporangia on the inoculation needle with sterile water, then use Vortex with a rotating speed of 1500rpm / min for 30s, repeat twice; then adjust the concentration of the sporangia suspension with a hemocytometer to adjust the concentration of the sporangia suspension to 1 / 2ul;

[0026] (2) Place three coverslips that have been sterilized with 75% ethanol on a glass slide, drop a drop of sporangia suspension on the three coverslips respectively, and then inspect under a 10×10 times microscope. To a single sporangia, add a 0.8cm×0.8cm rye culture medium on the droplet, then transfer it to a sterilized empty petri dish, and seal it;

[0027] (3) Put the petri dish in a refrigerator at 4°C for 2 hours at low temperature to stimulate the sporangia to release zoospores, then transfer to an ...

Embodiment 3

[0030] (1) Pick a little mycelium from the diseased potato leaves with an inoculation needle, stir it several times in a 2ml centrifuge tube containing 200ul sterile water, and mix the mycelia and sporangia on the inoculation needle with sterile water, then use Vortex with a rotating speed of 1500rpm / min for 30s, repeat twice; then adjust the concentration of the sporangia suspension with a hemocytometer to adjust the concentration of the sporangia suspension to 1 / 2ul;

[0031] (2) Place three coverslips that have been sterilized with 75% ethanol on a glass slide, drop a drop of sporangia suspension on the three coverslips respectively, and then inspect under a 10×10 times microscope. To a single sporangia, add a 0.8cm×0.8cm rye culture medium on the droplet, then transfer it to a sterilized empty petri dish, and seal it;

[0032] (3) Put the petri dish in a refrigerator at 4°C for 2 hours at low temperature to stimulate sporangia to release zoospores, then transfer to an envi...

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Abstract

The invention belongs to the field of microorganisms, and particularly relates to a method for separating monosporangiums of potato late blight pathogens. On the basis of an existing suspension liquid separating method, the monosporangiums are separated through the characteristic that each monosporangium contains a plurality of zoospores, and genetic materials of the zoospores are the same; the monosporangiums are stimulated at the low temperature to release the zoospores, and the new method for separating the monosporangiums is formed. The success rate of the improved method is nearly 6 times that of the original method, and operation time is halved; meanwhile, operative difficulty is greatly reduced, and the method is an effective method for rapidly separating a large mass of monosporangiums in a pathogeny-group researching test.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a method for isolating monosporangia of potato infestans. Background technique [0002] Potato late blight is the most important disease in potato production, and it is also one of the most classic diseases in plant pathology. The pathogen causing the disease is Phytophthora infestans. Potato-producing areas all over the world have occurred, and the epidemic year generally reduces production by 30%. In the 1840s, a large number of Irish potatoes died, and the production was cut in half, causing more than 1 million people to starve to death and 2 million people to emigrate overseas. At that time, there were various speculations about the cause of potato death. In 1842, von Martius first believed that it was caused by pathogens. In 1857, Speerschneider proved that mold on leaves could cause tuber rot. From 1861 to 1863, DeBary (deBary) determined that the lesions on the ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00
CPCC12N1/14C12N3/00
Inventor 谢业焜吴娥娇詹家绥李冬亮靳宇佳
Owner FUJIAN AGRI & FORESTRY UNIV
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