38 results about "Potato late blight fungus" patented technology

The invention relates to a strain of lys-starch bacillus as well its application. The bacterium is separated from the soil on eggplant fields, anning district, Lanzhou, Gansu. The bacterium is identified to be Bacillus amyloliquefaciens. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus amyloliquefaciens I7 and preserving code of M206027. The broth of M206027 and M206027strain, non-cell culture fluid of M206027 strain's broth, and the monomer extracted from the M206027strain, M206027 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

The invention relates to a strain of Bacillus subtilis as well its application. The bacterium is separated from the soil on lily fields, fanjiaping, Lanzhou, Gansu. The bacterium is identified to be Bacillus-subtilis. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus-subtilis M22BH3 and preserving code of M206028. The broth of M22BH3 and M22BH3 strain, non-cell culture fluid of M22BH3 strain's broth, and the monomer extracted from the M22BH3 strain, M22BH3 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

The invention disclose a pyridine salt compound containing 1,3,4-oxadiazolyl (thiadiazolyl) and a preparation method and application thereof. The compound is of the structure shown in the general formula (I). Based on the 1,3,4- oxadiazole (thiadiazole) thioether (sulfoxide or sulfone) compound, a pyridine base capable of improving the biological activity of the target compound is introduced into the system, and a series of 1,3,4- oxadiazole (thiadiazole) oxygen ether, thioether, sulfoxide or sulfone amphipathic molecules containing pyridine salt are synthesized. It is found that the compound has the good restraining effect on pathogenic bacteria and fungi such as Xanthomonas oryzae pv. oryzae, Ralstonia solanacearum, Xanthomonas axonopodis pv. citri, Gibberella zeae, Phytophythora infestans, Rhizoctonia solani, Borrytis cinerea, Fusarium oxysporum and Phytophythora infestans, and provides an important scientific foundation for new pesticide research, development and creation.

The invention discloses a Streptomyces albus MC-15 bacterial strain as well as a method and application thereof for preparing fermentation liquor thereby. The preservation number of the Streptomyces albus MC-15 bacterial strain is CGMCC NO.3652. The method comprises the following steps of: transplanting bacterial strains stored on a Gause No.1 inclined culture medium to a Gause NO.1 flat culture medium for activated cultivation; then inoculating the product into a Gause No.1 culture medium with 50mL liquid for the cultivation of seed liquor; then inoculating the product into a 250mL triangular flask in a Gause No.1 culture medium with 100mL liquid for intermediate cultivation; and then filtering and degerming the liquor to obtain the fermentation liquor. The fermentation liquor can not only exhibit the effect of restraining the growth of hypha of potato phytophtorainfestans strongly, but also has obvious effect in promoting the germination of seed potatoes, the growth of seedlings and the growth of plants, resisting phytophtorainfestans and increasing the yield.

The invention relates to a heterocycle substituted 1,3,4-oxadiazole compound and a preparation method and application thereof. The compound includes the following steps shown in the general formula (I) in the description. The compound has a good inhibitory effect on pathogenic bacteria and fungi, such as rice white leaf diseases, citrus canker diseases, pepper wilt pathogenic bacteria, blueberry phytophthora lateralis, wheat gibberella saubinetii, potato phytophthora infestans, oilseed rape sclerotinia sclerotiorum, dragon-fruit colletotrichum orbiculare and the like.

The invention relates to a preparation method for a carbazolylv isopropanolamine derivative with the chiral center and application. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwi fruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteria and the like.

The invention discloses streptomyces hygroscopicus capable of producing salicylic acid and rapamycin and application of the streptomyces hygroscopicus to prevention and control of plant oomycetes andfungal diseases. The invention also discloses the streptomyces hygroscopicus capable of producing the salicylic acid and the rapamycin, which has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 15518, and the application of the streptomyces hygroscopicus to the prevention and control of the plant oomycetes and fungal diseases. The invention identifiesa biocontrol bacterium aiming at the plant oomycetes and fungal diseases, especially phytophthora infestans and fusarium oxysporum; the bacterium can be used for remarkably inhibiting spore germination and mycelium growth of the phytophthora infestans; an effective method is provided for biologically preventing and controlling the plant oomycetes and fungal diseases, especially the phytophthora infestans and fusarium wilt. The method disclosed by the invention can be used for effectively preventing and controlling the phytophthora infestans, the fusarium wilt and other plant diseases and reducing the economic loss; the method has important meaning of reducing the utilization of pesticide and alleviating environment pollution, and also has significant application value of keeping the balance and sustainable development of an ecological system.

The invention discloses a phyrophthora infestans LAMP detection primer and a visual detection method thereof, and the phyrophthora infestans LAMP detection primer is specially used for specific detection of phyrophthora infestans. The phyrophthora infestans LAMP primer F3, B3, FIP and BIP is mainly designed. Green fluorescence or a ladder pattern with the LAMP characteristic feature can be observed through LAMP chromogenic reaction after isothermal amplification or agarose gel electrophoresis detection. The LAMP primer and the using method thereof can be used for fast, sensitively and accurately detecting plants infected by phyrophthora infestans, can be used for monitoring and authenticating of infestans and early-stage diagnosis of field disease damage and provides reliable technical and theoretical basis for prevention and control of potato late blight.

The invention relates to a process which induces potato late blight pathogen to generate pathogenic secretory protein, which comprises placing potato late blight pathogen sample in common water agar culture medium to hydrate and cultivate, selecting a little amount of bacterial filament to graft in the common solid culture medium through a grafting needle when hoary moldy layers grow on the leaves, cultivating in dark under 16-18 DEG C, transferring bacterial filament blocks into 100mL pathogenic protein liquid inducing medium after forming a larger bacterial colony, removing bacterial filament bodies by double-layer sterilizing filtering paper vibrating in 150 rpm for 14-16days under 18 DEG C, centrifuging the filtering liquid in 4000rpm for 10 min , adding ammonium sulfate (697g / L) in supernatant fluid, centrifuging in 10000rpm for 20min conducting dialysis treatment to the sedimentation, obtaining potato late blight pathogen pathogenic secretory protein abstract after freeze drying, and reserving under -20 DEG C. The process can induce potato late blight pathogen to generate a great amount of pathogenic secretory protein, fills the blank that pathogenic secretory protein can not be obtained in potato late blight pathogen pathogenic mechanism research at home and board, and has great significance.

The invention relates to a 1,3,4-oxadiazole thioether compound as well as a preparation method and application thereof. The compound has a structure shown as a general formula (I) in the description.The compound has a good inhibition effect on pathogenic bacteria and fungi. By aiming at xanthomonas oryzae pv.oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjac xanthomonas oryzae, xanthomonas axonopodis pv.citri, botryosphaeria dothidea, bacterial canker and wilt of tomato, pseudomonas syringae pv.actinidae, Leucostoma cincta(Fr.:Fr.)Hohn, B.cinerea, F.oxysporum, S.sclerotiorum,G.zeae, P.infestans, P.cinnamomi and the like, good inhibition effects are achieved.

The invention discloses a biocontrol inoculant for controlling potato late blight, and belongs to the technical field of crop disease control. The biocontrol inoculant is fermented by streptomyceseuropaeiscabiei St19. A strain is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) on March 24, 2014; and the preservation number is CGMCCNO.8962. The biocontrol inoculant can inhibit growth of potato phytophthora infestans, represents a better control effect on the potato late blight, can be used for root irrigation in a seedling stage or an adult-plant stage of a potato, or can be mixed with an organic fertilizer to prepare a microbial fertilizer. The St19 strain is obtained from potato rhizosphere soil, is harmonious and compatible with soil ecology, and is non-toxic and non-virulent, so that the strain has a larger application potential in biological control of the potato late blight.

The invention relates to an application of an isoflavonoids compound, that is, glabridin, to prepare an agricultural bactericide. The compound is used for inhibiting the following pathogenic funguses of crops: fusarium graminearum, colletotrichum gloeosporioides, botrytis cinerea, phytophthora infestans, or pyricularia grisea, and the following pathogenic bacteria of crops: erwina carotovora, pseudomonas solanacearum, ralstonia solanacearum, or pseudomonas syringae pv. actinidae. Furthermore, the compound can be prepared into various formulations with other agricultural auxiliaries, and the compound has a synergistic effect after combined with shikonin according to a certain weight ratio.

The invention relates to a preparation method for carbazolyl isopropanolamine derivative and application thereof. The structure of the compound is shown in the formula (I), and the compound achieves the better effect of inhibiting xanthomonas oryzae, ralstonia solanacearum, cucumber xanthomonas oryzae, konjak xanthomonas oryzae, xanthomonas citri, grape ulcer bacteria, tomato ulcer bacteria, kiwifruit ulcer bacteria, apple ulcer bacteria, cucumber botrytis cinerea, chili blight bacteria, sclerotinia sclerotiorum, wheat gibberella saubinetii, potato late blight, blueberry root rotting bacteriaand the like.

The invention provides a set of production technology for preventing ralstonia solanacearum from growing and reproducing in solanaceae crops to increase crop yield by aiming at the defects of the prior art for controlling the damages of the ralstonia solanacearum to the crops. Brevibacillus choshinensis X23 has a wide bacteriostatic spectrum and good environmental safety and has antagonism effects to various pathogenic fungi, such as phytophthora parasitica var nicotianae, pyricularia grisea, alternaria alternate, phytophthora infestans and the like, so that the brevibacillus choshinensis X23 has favorable development prospect and application potential.

The invention belongs to a method for preparing inula japonica plant extract and applications of the inula japonica extract. The plant material (root, stem, leaves, inflorescence and whole grass) of the inula japonica (including Inula japonica and Inula britannica) is crushed and extracted by a solvent which comprises 95% of alcohol; and the extract solution is concentrated to have the same weight with the inula japonica crushed matters, thus obtaining inula japonica extract. The inula japonica extract has obvious inhibition effects to cucumber botrytis cinerea, alternaria solani, cladosporium fulvum, colletotrichum lagenarium, sphaerotheca fuliginea, pseudoperonospora cubensis, potato phytophthora infestans, tomato phytophthora infestans and rhizoctonia cerialis; wherein the inflorescence extract has strong antibacterial activity. The inula japonica extract extracted by alcohol can be matched with suitable solvent and pesticide adjuvant to prepare britannica lactone emulsifiable solution and britannica lactione micro-emulsion of botanical fungicide. The inula japonica extract has obvious effects for preventing cucumber downy mildew, sphaerotheca fuliginea, tomato gray mold and tomato leaf mould, is especially suitable for prevention and control on the fungi diseases to economical crops such as vegetables and the like, and is a botanical fungicide with nuisanceless.

The invention discloses a preservation method of pathogenic bacteria of potato late blight and the special medium for the same. The preservation method of pathogenic bacteria of potato late blight comprises the following steps. After being inoculated into a vessel containing the mixture of aseptic rye kernels and water, pathogenic bacteria of potato late blight are preserved under dark and sealed condition at the temperature of 12-18 DEG C.. On the basis that the pathogenic bacteria of potato late blight can survive in water, the method of preserving pathogenic bacteria of potato late blight in the mixture of aseptic rye kernels and water is put forward in the invention. Experiments show that the activity of pathogenic bacteria of potato late blight is still high after long-term preservation. The method of the invention also has the advantages of easy operation, low cost and decreased pollution probabilities of undesired microbes, thereby providing the foundation for the research on pathogenic bacteria of potato late blight and diseases thereof.

The invention discloses a pyrazolyl-oxa(thia)diazole compound containing pyridinium salt, and a preparation method and application thereof. The compound has the structure as shown in the following general formula (I). On the basis of a pyrazolyl-oxa(thia)diazole thioether(oxoether) compound, a pyridinium group capable of improving the biological activity of a target compound is introduced into the system to synthesize a series of pyrazolyl-oxa(thia)diazole oxoether or thioether amphipathic molecules containing the pyridinium salt; moreover, the compound has a good inhibition effect on pathogenic bacteria and fungi, has good inhibition effects on bacteria (bacterial leaf blight of rice, ralstonia solanacearum, xanthomonas citri and the like) and fungi (gibberella zeae, phytophthora infestans, rhizoctonia solani, botrytis cinerea pers, fusarium oxysporum, apple rot pathogenic bacteria and the like), and provides an important scientific base for research, development and creation of novel pesticides.

The invention discloses the application of a compound Quinolactacide. The structure of the compound is shown in the formula (1); the compound has better inhibitory activity to the phytophthora infestans, and minimal inhibitory concentration (MIC) of 8mu g / mL can be used as a novel pesticide component or a lead compound with a bactericidal effect. The compound is a microorganism-derived natural product. Compared with a traditional chemical synthetic bactericide, the compound has the advantages of good environment compatibility and easiness in mass production.

The invention discloses a simple storage method for potato late blight bacteria. The method comprises the following steps: taking sterile water as a storage carrier for storing the potato late blightbacteria, specifically, pouring a solid medium into a culture dish, inoculating the potato late blight bacteria into the center of the solid medium, causing the potato late blight bacteria to grow andmultiply at 17 DEG C, transferring the grown potato late blight bacteria blocks into a triangular flask filled with sterile water and storing under 17 DEG C in a dark and sealed container, thereby realizing the simple, quick, medium and long term storage for the potato late blight bacteria. The storage method disclosed by the invention is characterized by simple operation, convenience, high speedand low cost; the treated potato late blight bacteria are long in storage period and stable in culture characters; the regular transplanting in the storage period is not required; the storage cost and space are saved, the storage is convenient and quick and the required culture can be stored at any time; and a basic guarantee is supplied for the biological characteristics of the potato late blight bacteria, the breeding for disease resistance of the potato and the research on diseases.

The invention belongs to the field of microorganisms, and particularly relates to a method for separating monosporangiums of potato late blight pathogens. On the basis of an existing suspension liquid separating method, the monosporangiums are separated through the characteristic that each monosporangium contains a plurality of zoospores, and genetic materials of the zoospores are the same; the monosporangiums are stimulated at the low temperature to release the zoospores, and the new method for separating the monosporangiums is formed. The success rate of the improved method is nearly 6 times that of the original method, and operation time is halved; meanwhile, operative difficulty is greatly reduced, and the method is an effective method for rapidly separating a large mass of monosporangiums in a pathogeny-group researching test.

The invention belongs to the technical field of plant breeding for disease resistance and in particular relates to a method for identifying phytophythora infestans physiological race and application thereof. The method for identifying phytophythora infestans physiological race disclosed by the invention comprises the following steps: (a) setting a disease nursery field; (b) culturing a potato hostmaterial on a culture medium, and performing water culture to form water culture seedlings; (c) transplanting the water culture seedlings into the disease nursery field in the step (a); and (d) performing disease investigation on potatoes in the step (c), and recording the type of the phytophythora infestans physiological race. The experimental study is performed by selecting natural disease nursery, the advantages of scientificity, effectiveness and environment friendliness are taken into account, the requirement on professional skill is low in the whole experimental process, the operation is simple and feasible, the latter half part of the experiment is transferred into a large field for identification, the experimental effect is obvious, the experimental duration is shortened, and theexperimental accuracy is improved.

The invention discloses a preparation method of a culture medium for an antagonistic test of phytophthora infestans. The preparation method comprises steps as follows: 1), preparing a PDA culture medium; 2), preparing an HX culture medium; 3), preparing a mixed culture medium to obtain the culture medium for the antagonistic test of phytophthora infestans. The invention further provides the culture medium and an application. The culture medium has the beneficial effects as follows: influence of the culture medium on microorganism growth state is taken into full consideration, the culture medium suitable for the antagonistic test of phytophthora infestans is obtained through determination for healthy growth of phytophthora infestans on the culture medium and determination for capability ofan antagonistic strain HC5 for producing secretory substances on the culture medium, and one new practical substrate is provided for screening work of the antagonistic strains of phytophthora infestans.

The invention specifically discloses a real-time fluorescent LAMP detection method and kit for Phytophthora infestans, belonging to the field of molecular biology. According to the invention, proper primers are screened out and a reaction system is optimized, so the real-time fluorescent LAMP detection method for Phytophthora infestans is convenient and fast, has high specificity and high sensitivity, and has the lowest detection limit of 371 pg / [mu]L, which is 10 times of the lowest detection limit of corresponding ordinary PCR; and results can be observed in 40 min. The detection method provided by the invention overcomes a plurality of problems such as complex detection process, high cost and low efficiency in conventional detection of Phytophthora infestans.

The invention provides a method for rapidly extracting DNA of a single sporangium of phytophythora infestans. The method comprises the steps that the single sporangium is obtained through the capillarity of a capillary glass tube, and then the DNA of the single sporangium is directly extracted through an improved CTAB method. Impurities such as protein and polysaccharide in the single sporangium are few, therefore, phenol-chloroform-isoamyl alcohol does not need to be utilized for removing the impurities, and losing of the DNA in the cumbersome operation process is greatly reduced. Pure DNA of the phytophythora infestans can be efficiently extracted within a short time.

The invention provides a bactericide composition, a controlled release system and applications thereof, wherein the active components of the bactericide composition and the controlled release system are allyl isothiocyanate and an aldehyde-based compound. According to the present invention, the bactericide composition and the controlled release system have good prevention and control effects on Phytophthora infestans, and can control Phytophthora infestans in a long-acting manner, reduce the use frequency and reduce the cost; by separately using allyl isothiocyanate or combining allyl isothiocyanate and a certain aldehyde-based compound, Phytophthora infestans can be effectively controlled, and the effective duration can be up to 3 months, such that a certain combination of allyl isothiocyanate and aldehyde can provide the synergistic effects in the prevention and control of Phytophthora infestans.

The invention provides a control method for phytophthora infestans and plant diseases caused by phytophthora infestans. The method includes applying allyl isothiocyanate and / or a controlled release system thereof, such as controlled release capsules, sustained release granules, wettable powder, and the like, to the soil for plants to be planted, especially before planting. The controlled release system of allyl isothiocyanate provided by the invention has good control effect on phytophthora infestans, and can control the disease for a long time, reduce application frequency, and reduce the application cost. The use of allyl isothiocyanate alone can effectively control phytophthora infestans and the lasting period can be up to 2 months.

The invention relates to the field of agricultural plant protection, and particularly relates to a sterilization compound and a preparation method and an application thereof. The compound has the structure represented by a formula defined in the specification, wherein R is selected from one of components defined in the specification; the compound has excellent bacteriostatic activity on potato phytophthora infestans, phytophthora capsici, phytophthora nicotianae, peronophthora litchii, pseudoperonospora cubensis and other plant pathogenic oomycetes, and has broad application prospects in prevention and control of oomycete diseases.

The invention belongs to the field of crop disease prevention and control and discloses an antibiological inoculant for preventing and treating potato late blight. A preparation method of the antibiological inoculant comprises: selecting high-quality commercially available virus-free potatoes and commercially available bacillus subtilis powder, activating the commercially available bacillus subtilis powder through inoculating a culture liquid with the commercially available bacillus subtilis powder and carrying out shake cultivation at 30 DEG C at a rotation rate of 150r / min for 36h to obtaina bacillus subtilis powder seed liquid for next use, carrying out fermentation through inoculating a fermentation medium with the seed liquid and fermenting the seed liquid at 30 DEG C at a rotation rate of 150r / min for 7 days to obtain fermentation broth, measuring the activity of the fermentation broth through centrifuging the fermentation broth at a rotation rate of 5000r / min for 5min, carryingout filtration, collecting the treated fermentation broth and measuring the bacteriostatic activity of the fermentation broth through potato late blight as a target, and collecting the fermentation broth through detecting the fermentation broth and collecting the qualified fermentation broth. The antibiological inoculant does not cause any pollution to the environment and has good prevention andtreatment effects on potato late blight.

The invention relates to a phytophthora infestans culture medium and a preparation method thereof, and belongs to the field of ovum bacterium culture. The phytophthora infestans culture medium comprises mixed liquid consisting of rye juice, tomato juice and calcium carbonate, and agar strips. The preparation method comprises specific preparation steps of weighing rye, adding an appropriate amountof water, performing sterilization in a high-pressure sterilization pot, adding water to the sterilized rye, performing crushing, performing boiling over, and performing filtration so as to obtain therye juice; cutting tomatoes into dices, performing crushing to obtain tomato original juice, and mixing the tomato original juice with clean water in the volume ratio being 1 to 1 so as to obtain thetomato juice; adding the calcium carbonate to the tomato juice in proportion, then mixing the rye juice with the tomato juice in proportion to obtain the mixed liquid, adding the agar strips to the mixed liquid in proportion, adjusting pH value with a 0.1% sodium hydroxide solution, and performing sterilization so as to complete the preparation of the culture medium. The culture medium disclosedby the invention can effectively promote growth of the phytophthora infestans, the spore production capacity of the phytophthora infestans can be increased, the spore production number is obviously increased, the preparation method is simple and the preparation time is short.