Method for identifying phytophythora infestans physiological race and application thereof

A potato late blight, potato late blight technology, applied in biochemical equipment and methods, microbial determination/inspection and other directions, can solve the problems of high identification technology requirements, cumbersome operation, heavy workload, etc., and achieves accelerated experimental progress and operation. Simple and feasible, the effect of improving the accuracy of the experiment

Active Publication Date: 2018-12-07
ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the experimental process of identifying the physiological race of Potato infestans, Potato infestans is isolated and purified after sampling the disease samples. To identify the host, the detoxified tissue culture seedlings are usually cultivated in the room for 1-3 months before the leaves can be cut. , and each experiment needs to be repeated 3-5 times, and the experiment is guaranteed to be free from pollution. The existing identification technology of the physiological race of P. longer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying phytophythora infestans physiological race and application thereof
  • Method for identifying phytophythora infestans physiological race and application thereof
  • Method for identifying phytophythora infestans physiological race and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A method for identifying physiological races of Phytophthora infestans, comprising the following steps:

[0070] (a) Set up disease gardens: select representative fields with high incidence of potato late blight in seven provinces: Liaoning, Jilin, Heilongjiang, Shandong, Henan, Hebei and Shaanxi;

[0071] (b) Rapid Propagation in Hydroponics: The virus-free test-tube plantlets of 12 kinds of standard identification hosts from R0 to R11 were cut indoors and cultured on MS medium at a temperature of 22°C. The cultivation room is used for rapid propagation of cuttings, and after 10 days of growth, roots will form strong hydroponic seedlings;

[0072] (c) Hydroponic seedlings enter the diseased nursery: transplant the hydroponic seedlings identifying the host to the diseased nursery field in step (a), and transplant 10 plants for each identified host in each region;

[0073] (d) Disease investigation: during the epidemic period of potato late blight, with the incidence of...

Embodiment 2

[0079] A method for identifying physiological races of Phytophthora infestans, comprising the following steps:

[0080] (a) Set up disease nursery fields: select representative fields with high incidence of potato late blight in Jiangxi, Inner Mongolia, Sichuan, Yunnan, Guizhou, Hubei and Chongqing;

[0081] (b) Rapid Propagation in Hydroponics: The virus-free test-tube plantlets of 12 kinds of standard identification hosts from R0 to R11 were cut indoors and then cultured on MS medium at a temperature of 28°C. The cultivation room is used for rapid propagation of cuttings, and after 15 days of growth, roots will form strong hydroponic seedlings;

[0082] (c) Hydroponic seedlings enter the disease nursery: transplant the hydroponic seedlings of the identified host to the diseased nursery field in step (a), and transplant 7 plants for each identified host in each region;

[0083] (d) Disease investigation: during the epidemic period of potato late blight, with the incidence of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of plant breeding for disease resistance and in particular relates to a method for identifying phytophythora infestans physiological race and application thereof. The method for identifying phytophythora infestans physiological race disclosed by the invention comprises the following steps: (a) setting a disease nursery field; (b) culturing a potato hostmaterial on a culture medium, and performing water culture to form water culture seedlings; (c) transplanting the water culture seedlings into the disease nursery field in the step (a); and (d) performing disease investigation on potatoes in the step (c), and recording the type of the phytophythora infestans physiological race. The experimental study is performed by selecting natural disease nursery, the advantages of scientificity, effectiveness and environment friendliness are taken into account, the requirement on professional skill is low in the whole experimental process, the operation is simple and feasible, the latter half part of the experiment is transferred into a large field for identification, the experimental effect is obvious, the experimental duration is shortened, and theexperimental accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of plant disease resistance breeding, in particular to a method for identifying physiological races of Phytophthora infestans and its application. Background technique [0002] Potato late blight is a typical airborne disease caused by Phytophthora infestans, which can spread rapidly when the temperature and humidity are suitable. The pathogen usually reproduces asexually; when two mating-type strains (A1 and A2) are present, they also reproduce sexually via oospores. With the migration of A2 mating type, many physiological races with stronger virulence appeared, which enhanced the disease resistance of potato infestation pathogen and aggravated the occurrence and prevalence of the disease. [0003] According to incomplete statistics, the physiological races of P. infestans infestans around the world are prone to change and tend to be complicated, and the probability and species of occurrence in different m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 肖春芳王甄张等宏沈艳芬高剑华张远学闫雷
Owner ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap