Preservation method of pathogenic bacteria of potato late blight and special medium for the same

A technology of potato late blight and preservation method, which is applied in the field of preservation of potato late blight pathogenic bacteria, can solve the problems of high preservation cost, increased pollution, difficulty in long-term preservation, etc., and achieve long preservation time, simple operation and low cost Effect

Inactive Publication Date: 2011-03-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to preserve the bacteria for a long time in vitro
The general storage method, such as the slope preservation method, because the medium used to prepare the slope is easy to dry, the storage time is usually less than one year, and usually the medium needs to be sterilized when preparing the slope, and then the medium is divided. work, increasing the chance of contamination
Spore freeze-drying storage method, liquid nitrogen storage method, etc. require special equipment, and the storage cost is also high

Method used

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  • Preservation method of pathogenic bacteria of potato late blight and special medium for the same
  • Preservation method of pathogenic bacteria of potato late blight and special medium for the same
  • Preservation method of pathogenic bacteria of potato late blight and special medium for the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, isolation culture and preservation of potato infestans

[0045] 1. Collection of samples of potato late blight and isolation and cultivation of pathogens

[0046] A total of 100 strains of P. infestans were isolated from Gansu Province, the main potato producing area in China. The specific method is: the diseased plants collected in the field ( figure 1) added to dry newspaper and brought back to the laboratory. In order to ensure the humidity, pour the newly prepared water agar medium (1.6%) into disposable 9cm plastic petri dishes, pour about 12-14mL medium into each dish, then let it cool in the ultra-clean bench to make it solidify. Take out the diseased samples taken back, select the leaves that are infected by pathogenic bacteria and still contain healthy parts; adjust the size of tap water to flow down in small streams, and rinse the leaves, especially the back of the leaves (mainly to wash off the zoospores produced on the back of the leaves sac...

Embodiment 2

[0065] Example 2 Bacterial strain preservation viability detection

[0066] (1) Determination of the survival rate of P. infestans after preservation

[0067] Pick one grain of rye from the 2mL cryopreservation tubes with storage time of 2 years and 1 year respectively in method II of Example 1 and put it on a 9cm flat plate containing rye tomato medium, and place it in the dark at 18°C After culturing for 7 days, observe under a microscope whether new hyphae or zoosporangia grow. Bacteria that grow new hyphae or zoosporangia are judged to be viable. In this way, the bacteria in 100 preservation tubes were detected respectively, and the ratio of the number of surviving tubes to the number of 100 tubes was counted. The experiment was repeated 3 times, and the results were averaged. Results 90% of the bacteria in the tubes kept for 2 years were alive; 90% of the bacteria in the tubes kept for 1 year were alive.

[0068] The detection results of the survival rate of the bacte...

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Abstract

The invention discloses a preservation method of pathogenic bacteria of potato late blight and the special medium for the same. The preservation method of pathogenic bacteria of potato late blight comprises the following steps. After being inoculated into a vessel containing the mixture of aseptic rye kernels and water, pathogenic bacteria of potato late blight are preserved under dark and sealed condition at the temperature of 12-18 DEG C.. On the basis that the pathogenic bacteria of potato late blight can survive in water, the method of preserving pathogenic bacteria of potato late blight in the mixture of aseptic rye kernels and water is put forward in the invention. Experiments show that the activity of pathogenic bacteria of potato late blight is still high after long-term preservation. The method of the invention also has the advantages of easy operation, low cost and decreased pollution probabilities of undesired microbes, thereby providing the foundation for the research on pathogenic bacteria of potato late blight and diseases thereof.

Description

technical field [0001] The invention relates to a preservation method and a special medium for potato late blight pathogenic bacteria. Background technique [0002] Potato is the fourth largest food crop in China and can be grown in Northeast, Northwest, Southwest, North China and other regions. Since 2005, China's potato production has ranked first in the world. Late blight is one of the most important diseases in potato and tomato production. It occurs in all major potato producing areas in my country. It occurs all year round in Southwest China due to climate reasons. In other areas, it also occurs at different times due to climate differences. It is distributed in 3- October. The disease is a soil-borne disease, which is frequently infested in the same growing season, and can infect the leaves, stems and tubers of the host, leading to the death of the whole plant within a week. In the early stage of infection, the leaves appear as water-soaked lesions, and a white fros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/645
Inventor 国立耘朱小琼韩淼
Owner CHINA AGRI UNIV
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