Preservation method of pathogenic bacteria of potato late blight and special medium for the same

Abstract

The invention discloses a preservation method of pathogenic bacteria of potato late blight and the special medium for the same. The preservation method of pathogenic bacteria of potato late blight comprises the following steps. After being inoculated into a vessel containing the mixture of aseptic rye kernels and water, pathogenic bacteria of potato late blight are preserved under dark and sealed condition at the temperature of 12-18 DEG C.. On the basis that the pathogenic bacteria of potato late blight can survive in water, the method of preserving pathogenic bacteria of potato late blight in the mixture of aseptic rye kernels and water is put forward in the invention. Experiments show that the activity of pathogenic bacteria of potato late blight is still high after long-term preservation. The method of the invention also has the advantages of easy operation, low cost and decreased pollution probabilities of undesired microbes, thereby providing the foundation for the research on pathogenic bacteria of potato late blight and diseases thereof.

Description

technical field [0001] The invention relates to a preservation method and a special medium for potato late blight pathogenic bacteria. Background technique [0002] Potato is the fourth largest food crop in China and can be grown in Northeast, Northwest, Southwest, North China and other regions. Since 2005, China's potato production has ranked first in the world. Late blight is one of the most important diseases in potato and tomato production. It occurs in all major potato producing areas in my country. It occurs all year round in Southwest China due to climate reasons. In other areas, it also occurs at different times due to climate differences. It is distributed in 3- October. The disease is a soil-borne disease, which is frequently infested in the same growing season, and can infect the leaves, stems and tubers of the host, leading to the death of the whole plant within a week. In the early stage of infection, the leaves appear as water-soaked lesions, and a white fros...

AI Technical Summary

Problems solved by technology

It is difficult to preserve the bacteria for a long time in vitro

The general storage method, such as the slope preservation method, because the medium used to prepare the slope is easy to dry, the storage time is usually less than one year, and usually the medium needs to be sterilized when preparing the slope, and then the medium is divided. work, increasing the chance of contamination

Spore freeze-drying storage method, liquid nitrogen storage method, etc. require special equipment, and the storage cost is also high

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