Phytophthora infestans culture medium and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of potato late blight and culture medium, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of unsatisfactory sporulation quantity of late blight bacteria, etc., and achieves improvement of sporulation ability and production time. The effect of short, simple preparation method
Inactive Publication Date: 2019-07-23
YUNNAN AGRICULTURAL UNIVERSITY
View PDF6 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, in the experiment, the number of spores produced by the artificial culture of P. infestans is not ideal, especially after long-term multi-generational cultivation, the number of sporangia produced by culture is difficult to meet the needs of the experiment, forming a bottleneck for research.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0017] A kind of preparation method of potato infestans infestans culture medium:
[0018] 1) Preparation of rye juice: Weigh 50-60g of rye, add appropriate amount of water, sterilize in an autoclave for 30 minutes, add 1200ml of water to the sterilized rye, break it, boil it and filter to obtain rye juice;
[0019] 2) Preparation of tomato juice: cut the tomato into small pieces and smash it to make tomato juice, mix the tomato juice with clear water at a volume ratio of 1:1 to obtain tomato juice, and autoclave it for later use;
[0020] 3) Add 1.2g of calcium carbonate to every 300ml of tomato juice, then mix rye juice and tomato juice at a volume ratio of 1:1 to obtain a mixed solution, add 8.5g of agar strips to each 500ml of mixed solution, and use 0.1 % sodium hydroxide solution to adjust the pH value to 6.5-7.0, carry out secondary sterilization after subpackaging, and complete the preparation of the medium after 30 minutes of sterilization.
Embodiment 2
[0022] A kind of preparation method of potato infestans infestans culture medium:
[0023] 1) Preparation of rye juice: Weigh 50-60g of rye, add appropriate amount of water, sterilize in an autoclave for 30 minutes, add 1200ml of water to the sterilized rye, break it, boil it and filter to obtain rye juice;
[0024] 2) Preparation of tomato juice: cut the tomato into small pieces and smash it to make tomato juice, mix the tomato juice with clear water at a volume ratio of 1:1 to obtain tomato juice, and autoclave it for later use;
[0025] 3) Add 1.2g of calcium carbonate to every 300ml of tomato juice, then mix rye juice and tomato juice at a volume ratio of 2:3 to obtain a mixed solution, add 8.5g of agar strips to each 500ml of mixed solution, and use 0.1 % sodium hydroxide solution to adjust the pH value to 6.5-7.0, carry out secondary sterilization after subpackaging, and complete the preparation of the medium after 30 minutes of sterilization.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a phytophthora infestans culture medium and a preparation method thereof, and belongs to the field of ovum bacterium culture. The phytophthora infestans culture medium comprises mixed liquid consisting of rye juice, tomato juice and calcium carbonate, and agar strips. The preparation method comprises specific preparation steps of weighing rye, adding an appropriate amountof water, performing sterilization in a high-pressure sterilization pot, adding water to the sterilized rye, performing crushing, performing boiling over, and performing filtration so as to obtain therye juice; cutting tomatoes into dices, performing crushing to obtain tomato original juice, and mixing the tomato original juice with clean water in the volume ratio being 1 to 1 so as to obtain thetomato juice; adding the calcium carbonate to the tomato juice in proportion, then mixing the rye juice with the tomato juice in proportion to obtain the mixed liquid, adding the agar strips to the mixed liquid in proportion, adjusting pH value with a 0.1% sodium hydroxide solution, and performing sterilization so as to complete the preparation of the culture medium. The culture medium disclosedby the invention can effectively promote growth of the phytophthora infestans, the spore production capacity of the phytophthora infestans can be increased, the spore production number is obviously increased, the preparation method is simple and the preparation time is short.
Description
technical field [0001] The invention belongs to the field of oomycete culture, and in particular relates to a culture medium for potato infestans and a preparation method thereof. Background technique [0002] Potato late blight is caused by Phytophthora infestans (Mont.) de Bary, an oomycete. It is one of the devastating diseases of potatoes. It can cause a 10% to 20% yield reduction in a normal year, and a 50% yield reduction in a severe year. % ~ 70%, or even no harvest. [0003] In order to control the disease of potato infestation, scientists have conducted a lot of research. The research shows that the sporangia produced by the strain of potato infestation is the key to the disease cycle of potato infestation. Therefore, more in-depth research should be carried out on this disease, and Phytophthora spores should be extracted Sac is one of the necessary jobs. However, the number of spores produced by artificially cultivating P. infestans in experiments is not ideal, e...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more