Antibiological inoculant for preventing and treating potato late blight
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A technology of potato late blight and biocontrol agents, which is applied in the direction of chemicals, biocides, fungicides for biological control, etc. Control effect, inhibition of mycelial growth, easy operation effect
Inactive Publication Date: 2018-11-16
CHONGQING ACAD OF AGRI SCI
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[0006] (1) In the prior art, chemical pesticides are often used for potato late blight, which is easy to remain, and whether it is the staff in the early stage or the eaters in the later stage, it has a certain impact on its health;
[0007] (2) At present, chemical
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Embodiment 1
[0064] The influence of embodiment 1 inoculum size on bacterial strain fermentation effect
[0065] Step 1: Select high-quality commercially available virus-free potatoes; commercially available Bacillus subtilis powder;
[0066] Step 2: Activation of the Bacillus subtilis powder: inoculate the Bacillus subtilis powder in the culture solution, and culture it on a shaker at 30°C and 150r / min for 36 hours to prepare the Bacillus subtilis powder seed liquid for future use;
[0067] Step 3: Fermentation: Inoculate the seed solution in the fermentation medium, the inoculation volume is 2%, 4%, 6%, 8%, 10%, respectively, the amount of culture liquid is 250mL, put 60mL in the Erlenmeyer flask, and incubate at 30°C , fermented under the condition of 150r / min for 7 days to obtain a fermentation liquid;
[0068] Step 4: Determining the activity of the fermented liquid: centrifuging the fermented liquid at 5000r / min for 5 minutes and then filtering, collecting the processed fermented li...
Embodiment 2
[0072] The impact of embodiment 2 rotating speed on bacterial strain fermentation effect
[0073] Step 1: Select high-quality commercially available virus-free potatoes; commercially available Bacillus subtilis powder;
[0074] Step 2: Activation of the Bacillus subtilis powder: inoculate the Bacillus subtilis powder in the culture solution, and culture it on a shaker at 30°C and 150r / min for 36 hours to prepare the Bacillus subtilis powder seed liquid for future use;
[0075] Step 3: Fermentation: inoculate the seed solution in the fermentation medium, the inoculation volume is 6%, and the culture liquid volume is 250mL, put 60mL in the Erlenmeyer flask, at 30°C, 110r / min, 120r / min, 130r / min , 140r / min, and 150r / min were fermented for 7 days to obtain a fermented liquid;
[0076] Step 4: Determining the activity of the fermented liquid: centrifuging the fermented liquid at 5000r / min for 5 minutes and then filtering, collecting the processed fermented liquid, and using P. inf...
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Abstract
The invention belongs to the field of crop disease prevention and control and discloses an antibiological inoculant for preventing and treating potato late blight. A preparation method of the antibiological inoculant comprises: selecting high-quality commercially available virus-free potatoes and commercially available bacillus subtilis powder, activating the commercially available bacillus subtilis powder through inoculating a culture liquid with the commercially available bacillus subtilis powder and carrying out shake cultivation at 30 DEG C at a rotation rate of 150r/min for 36h to obtaina bacillus subtilis powder seed liquid for next use, carrying out fermentation through inoculating a fermentation medium with the seed liquid and fermenting the seed liquid at 30 DEG C at a rotation rate of 150r/min for 7 days to obtain fermentation broth, measuring the activity of the fermentation broth through centrifuging the fermentation broth at a rotation rate of 5000r/min for 5min, carryingout filtration, collecting the treated fermentation broth and measuring the bacteriostatic activity of the fermentation broth through potato late blight as a target, and collecting the fermentation broth through detecting the fermentation broth and collecting the qualified fermentation broth. The antibiological inoculant does not cause any pollution to the environment and has good prevention andtreatment effects on potato late blight.
Description
technical field [0001] The invention belongs to the technical field of crop disease prevention and control, and in particular relates to a biocontrol agent for preventing and treating potato late blight. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Potato late blight is caused by Phytophthora infestans, a devastating oomycete disease that causes potato stem and leaf death and tuber rot. Potato late blight is caused by Phytophthora infestans. When the leaves are infected, water-soaked green-brown spots will appear on the tip or edge of the leaves, with a light green halo around the spots. When the humidity is high, the spots will expand rapidly and turn brown, and produce a circle of white mold, namely cysts and spores. cysts, especially on the back of leaves; when dry, the lesions turn brown and dry, brittle and easy to crack, no white mold, and the expansion speed slows down. Brown streaks appea...
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