Culture medium for antagonistic test of phytophthora infestans as well as preparation method and application of culture medium

Abstract

The invention discloses a preparation method of a culture medium for an antagonistic test of phytophthora infestans. The preparation method comprises steps as follows: 1), preparing a PDA culture medium; 2), preparing an HX culture medium; 3), preparing a mixed culture medium to obtain the culture medium for the antagonistic test of phytophthora infestans. The invention further provides the culture medium and an application. The culture medium has the beneficial effects as follows: influence of the culture medium on microorganism growth state is taken into full consideration, the culture medium suitable for the antagonistic test of phytophthora infestans is obtained through determination for healthy growth of phytophthora infestans on the culture medium and determination for capability ofan antagonistic strain HC5 for producing secretory substances on the culture medium, and one new practical substrate is provided for screening work of the antagonistic strains of phytophthora infestans.

Description

technical field [0001] The invention relates to the technical field of microbial culture medium, in particular to a culture medium for antagonism test of potato infestans and its preparation method and application. Background technique [0002] Potato late blight is caused by Phytophthora infestans ( Phytophthora infestans (Mont.) de Bary) is a devastating disease in potato production, seriously affecting the yield and quality of potatoes. At present, the control of potato late blight mainly includes chemical control and disease-resistant breeding. Chemical control works quickly, but there are problems such as pesticide residues and environmental pollution; the application of disease-resistant breeding in potatoes is greatly limited, because the cultivated species of potatoes are autotetraploid asexually propagated crops, which are highly heterozygous. Due to the complex genetic type, it is incompatible with most diploid wild species. With the continuous development and ...

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Problems solved by technology

During the applicant's research, because the growth of Phytophthora infestans infestans requires a special culture substrate, it needs to grow healthy on the rye medium, and the applicant screened a strain with better antagonistic properties from the soil. Strain HC5 cannot produce antagonistic secretions on rye medium. Studies have found that it can produce antagonistic secretions better on the culture medium PDA medium, but P. infestans cannot produce spores on PDA medium.

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