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Embedding immobilization method for anaerobic ammoxidation mixed culture

An anaerobic ammonia oxidation and mixed culture technology, applied in anaerobic digestion treatment, chemical instruments and methods, fixed on/in organic carriers, etc., can solve the impact, loss of ANAMMOX mixed culture, ANANMMOX mixed culture It can solve the problems of destroying the reactor conditions and other problems, and achieve the effect of simple process, good physical stability and mechanical strength, and low cost.

Inactive Publication Date: 2008-05-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the anaerobic ammonium oxidation process with harsh operating conditions, if the ordinary activated sludge reflux technology is used to maintain its biomass, it will lead to the destruction of the ANANMMOX mixed culture and the impact on the reactor conditions, and will still make valuable ANAMMOX Mixture Loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Treatment of nitrogenous wastewater under batch conditions using immobilized anammox co-cultures. The specific process is: prepare a mixed solution of 8wt% polyvinyl alcohol (PVA) and 2wt% sodium alginate (SA) as an embedding agent, and then mix it with anammox sludge in a ratio of 2:1 (anammox The sludge is centrifuged after being washed with phosphate buffer solution with a pH of 7.0), and the above-mentioned mixed solution is added dropwise to 4wt% CaCl through a No. 9 injection needle with a peristaltic pump. 2 Solution (CaCl 2 The volume of the solution is more than 3 times the mixing volume of the embedding agent and the sludge) as a cross-linking agent, the pellets are rapidly formed, and the pellets have a particle size between 3 and 5 mm. In an anaerobic environment, crosslink in a 4°C refrigerator for 12 hours. Sufficient cross-linking time helps the embedding agent and the cross-linking agent to fully cross-link, improving the stability and physical strengt...

Embodiment 2

[0021] Using immobilized anammox mixed cultures to treat nitrogenous wastewater under continuous culture conditions. The specific process is: prepare a mixed solution of 10wt% PVA and 2wt% SA as an embedding agent, mix it according to a ratio of 7:1, and then mix it with anammox sludge according to a ratio of 1:1 (anammox sludge After washing with phosphate buffer solution with a pH of 6.0 and centrifuging), add dropwise to 6wt% CaCl 2 Solution (CaCl 2 The volume of the solution is more than 3 times the mixing volume of the embedding agent and the sludge) as a cross-linking agent, the pellets are rapidly formed, and the pellets have a particle size between 3 and 5 mm. In an anaerobic environment, crosslink in a refrigerator at 4°C for 8 hours. Sufficient cross-linking time helps the embedding agent and the cross-linking agent to fully cross-link, improving the stability and physical strength of the pellets (the breakage rate of the pellets is less than 2% after stirring with...

Embodiment 3

[0024] Treatment of nitrogenous wastewater under batch conditions using immobilized anammox co-cultures. The specific process is: prepare a mixed solution of 8wt% PVA and 1wt% SA as an embedding agent, and then mix it with the anammox sludge at a ratio of 1.2:1 (the anammox sludge is passed through a phosphate buffer solution with a pH of 8.0 Centrifugal after cleaning), the above-mentioned mixed solution is added dropwise to 5wt% CaCl through the No. 9 injection needle with a peristaltic pump 2The solution (volume more than 3 times the volume of the mixed embedding agent and sludge) is used as a cross-linking agent, and the pellets are formed rapidly, with a particle size between 3 and 5 mm. In a completely anaerobic environment, crosslink in a refrigerator at 4°C for 24 hours. Sufficient cross-linking time helps the embedding agent and the cross-linking agent to fully cross-link, improving the stability and physical strength of the pellets (the breakage rate of the pellets ...

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Abstract

The invention discloses an entrapping immobilization method of mixed anaerobic ammonium oxidation culture, which comprises the steps of obtaining embedding medium, mixing the embedding medium and sludge, obtaining immobilized anaerobic ammonium oxidation beads, activating the immobilized anaerobic ammonium oxidation beads, and the like. The obtaining of the embedding medium is to weigh polyvinyl alcohol and sodium alginate to be made into solution, according to the mass percent, the concentration of the polyvinyl alcohol is 6%-10wt% and the concentration of the sodium alginate is 1%-2wt%, and the polyvinyl alcohol and the sodium alginate are well stirred to make into mixed embedding medium. The method is simple in technique, simple in operation and low in costs. The entrapping of immobilized ANAMMOX mixed culture can be successful, the method can design an anaerobic ammonia oxidation reactor which is highly effective and specific in action, and the anaerobic ammonia oxidation reactor and a lead nitrosation reactor can be combined to form a high-efficiency stable biological denitrification system, which is adaptable for commercial manufacture and can bring about perfect economic and environmental benefits.

Description

technical field [0001] The invention relates to the fields of environmental engineering, water pollution control and ecological restoration engineering, in particular to the realization of an immobilized anaerobic ammonium oxidation process. Background technique [0002] In 1990, the Kluyver Biotechnology Laboratory of the Delft University of Technology in the Netherlands discovered the phenomenon of anaerobic ammonia oxidation for the first time, and soon became a research hotspot in the field of water treatment all over the world. Compared with the traditional nitrification-denitrification biological denitrification method, the anammox process has the following advantages: the anammox bacteria are autotrophic bacteria, and there is no need to add organic matter as an electron donor, which can save costs and prevent secondary denitrification. Pollution; in the anaerobic ammonium oxidation reaction, only ammonia needs to be oxidized into nitrite. Compared with the traditiona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C02F3/28C02F3/34
Inventor 胡勇有朱刚利
Owner SOUTH CHINA UNIV OF TECH
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