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Microbiological additive for ruminants and preparation technology thereof

A technology for ruminants and additives, which is applied in the field of microecological additives and its preparation, can solve problems such as the efficacy of metabolites that are not involved, and achieve the effects of increasing feed conversion rate of dairy cows, increasing fattening weight gain of lambs, and increasing milk production

Inactive Publication Date: 2011-11-02
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There are also records of related ruminant feed additives and their preparation methods in the prior art, for example: Patent ZL200810103485.1: Composite microbial additives for dairy cattle feed and its preparation method, its characteristics mainly describe three kinds of bacteria (Bacillus subtilis natto, feces Enterococcus and Saccharomyces cerevisiae) and their synergistic effect, not related to the efficacy of the metabolites produced by the three bacteria

Method used

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  • Microbiological additive for ruminants and preparation technology thereof
  • Microbiological additive for ruminants and preparation technology thereof
  • Microbiological additive for ruminants and preparation technology thereof

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Embodiment 1

[0030] (1) Strain recovery culture: Saccharomyces cerevisiae, Candida utilis and Bacillus subtilis were respectively inoculated on slant surfaces, cultured at 30°C for 48 hours, then stored at 4°C, and subcultured once every 2 months;

[0031] (2) Bacterial strain expansion cultivation: inoculate step (1) recovery culture strain into the Erlenmeyer flask for expansion cultivation, and the concentration of the bacterial solution after the expansion cultivation is greater than 10 9 cfu / mL;

[0032](3) The strains are subjected to liquid enrichment fermentation respectively: inoculate the bacterium liquid cultivated in step (2) into the liquid medium with an inoculum size of 5% by volume fraction, and cultivate at 30°C for 18 hours; wherein Saccharomyces cerevisiae and urin The liquid culture medium of Candida includes the following weight ratio raw materials, glucose 1%, ammonium sulfate 0.5%, yeast extract 4%, KH 2 PO 4 0.4%, MgSO 4 0.01%, CaCl 2 0.01%, KCl 0.06%, the rest ...

Embodiment 2

[0041] (1) Strain recovery culture: Saccharomyces cerevisiae, Candida utilis and Bacillus subtilis were respectively inoculated on slant surfaces, cultured at 30°C for 48 hours, then stored at 4°C, and subcultured once every 2 months;

[0042] (2) Bacterial strain expansion culture: inoculate step (1) recovery culture strain into the Erlenmeyer flask to expand culture, the concentration of bacteria liquid after the expansion culture is greater than 10 9 cfu / mL;

[0043] (3) The strains are subjected to liquid enrichment fermentation respectively: inoculate the bacterium liquid cultivated in step (2) into the liquid medium with an inoculum size of 8% by volume fraction, and cultivate it for 22 hours at 32°C; The liquid culture medium of Candida includes the following weight ratio raw materials, glucose 2%, ammonium sulfate 1%, yeast extract 3%, KH 2 PO 4 0.3%, MgSO 4 0.03%, CaCl 2 0.02%, KCl 0.04%, the rest is water, the liquid medium for cultivating Bacillus subtilis inclu...

Embodiment 3

[0052] (1) Strain restoration culture: Saccharomyces cerevisiae, Candida utilis and Bacillus subtilis were respectively inoculated on slant surfaces, cultured at 30°C for 48 hours, then stored at 4°C, and subcultured once every 3 months;

[0053] (2) Bacterial strain expansion cultivation: inoculate step (1) recovery culture strain into the Erlenmeyer flask for expansion cultivation, and the concentration of the bacterial solution after the expansion cultivation is greater than 10 9 cfu / mL;

[0054] (3) The bacterial strains are subjected to liquid enrichment fermentation respectively: inoculate the bacterium solution cultivated in step (2) into the liquid medium with an inoculum volume fraction of 10%, and cultivate it for 26 hours at 33° C.; The liquid culture medium of Candida includes the following weight ratio raw materials, glucose 2.5%, ammonium sulfate 1.5%, yeast extract 2%, KH 2 PO 4 0.2%, MgSO 4 0.05%, CaCl 2 0.04%, KCl 0.02%, the rest is water, the liquid mediu...

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Abstract

The invention relates to a microbiological additive for ruminants and a preparation technology thereof. The additive is characterized by culturing respectively Saccharomyces cerevisiae, Candida utilis and Bacillus subtilis in a liquid culture medium to obtain Saccharomyces cerevisiae fermentation bacterial liquid, Candida utilis fermentation bacterial liquid and Bacillus subtilis fermentation bacterial liquid, mixing the three fermentation bacterial liquids, and then carrying out a fermentation process of the three fermentation bacterial liquids on a solid culture medium to obtain a mixed culture, wherein in the mixed culture, a polypeptide content is equal or greater than 5.3 g / Kg; a beta-glucan content is equal or greater than 125 mg / Kg; by weight, an amino acid content is equal or greater than 16.5%; and by weight, an organic acidity content is equal or greater than 3.45%. The invention also relates to a preparation method of the microbiological additive for ruminants.

Description

technical field [0001] The invention relates to a micro-ecological additive and a preparation process thereof, in particular to a ruminant micro-ecological additive capable of effectively increasing the milk yield of cows, reducing the number of milk somatic cells and increasing the weight gain rate of mutton sheep and the preparation process thereof. Background technique [0002] For many years, animal nutritionists and microbiologists have been committed to the research of rumen micro-ecosystem of ruminants, with the purpose of improving the production efficiency of ruminant livestock by regulating the rumen microflora. In recent years, the use of probiotics to regulate rumen function has become a research hotspot in this field. [0003] Domestic research reports on microecological preparations mainly focus on monogastric animals and poultry, while there are fewer reports on ruminants. Many studies have applied the mature technology of monogastric animals to ruminants. Du...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16A23K10/18A23K40/00A23K50/10
Inventor 刘大程王晓宏殷兆丽高民卢德勋
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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