Process for producing fermented soybean meal through solid state fermentation of mixed culture
A technology for fermented soybean meal and solid-state fermentation, which is applied in the field of solid-state fermentation of mixed strains to produce fermented soybean meal, can solve the problems of heat-sensitive material loss, unfavorable large-scale production, and reduced product nutrition, so as to reduce operation and time, promote further proliferation, and ensure The effect of viable bacterial content
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preparation example Construction
[0031] (1) Preparation of bacterial strain:
[0032] The following raw materials are added to the batching tank: barley malt, peptone, agar, sodium citrate, potassium dihydrogen phosphate, magnesium sulfate, sodium bicarbonate and water, and the mass ratio of the raw materials is barley malt: peptone: agar: citric acid Sodium: potassium dihydrogen phosphate: magnesium sulfate: sodium bicarbonate: water = 2.0-3.0: 1.5-1.8: 2.0-3.0: 0.5-1.0: 0.3-0.4: 0.015-0.020: 0.5-0.8: 180-220, heated to Boil, adjust the pH value to 4.5-5.5 and transfer it to the saccharification tank, add 9×10 to the saccharification tank 5 U-10×10 5 The saccharifying enzyme of U is kept at 55-65°C for 3-5 hours to obtain a saccharification solution, and the obtained saccharification solution is transferred into a fluid heater and heated to 95-100°C, incubated for 0.5 hours, and then cooled to 35°C in 5-10 seconds. -45°C, the breeding medium was obtained and added to the four chemostats;
[0033] The str...
Embodiment 1
[0051] This embodiment includes the following steps:
[0052] (1) Preparation of bacterial strain:
[0053] By following raw material: barley malt 2.5kg, peptone 1.7kg, agar 2.5kg, sodium citrate 0.8kg, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.018kg, sodium bicarbonate 0.7kg and water 190kg are added in the batching tank, heated To boiling, adjust the pH value to 4.8 and transfer to the saccharification tank, add 9×10 to the saccharification tank 5 The saccharifying enzyme of U was incubated for 3 hours at 65°C for saccharification to obtain a saccharification solution, and the obtained saccharification solution was transferred into a fluid heater and heated to 100°C, incubated for 0.5 hours, and then cooled to 40°C within 6 seconds to obtain a breeding medium, and added to four chemostats;
[0054] The strains of lactic acid bacteria, bifidobacteria, Bacillus subtilis and Candida utilis cultivated in the laboratory were respectively inserted into four chemo...
Embodiment 2
[0059] This embodiment includes the following steps:
[0060] (1) Preparation of bacterial strain:
[0061] The following raw materials: barley malt 2.4kg, peptone 1.6kg, agar 2.4kg, sodium citrate 0.8kg, potassium dihydrogen phosphate 0.36kg, magnesium sulfate 0.019kg, sodium bicarbonate 0.6kg and water 200kg are added to the batching tank, heated To boiling, adjust the pH value to 5.0 and transfer to the saccharification tank, add 9.6×10 to the saccharification tank 5 The saccharifying enzyme of U was incubated for 4 hours at 60°C for saccharification to obtain a saccharification solution, and the obtained saccharification solution was transferred into a fluid heater and heated to 95°C, kept for 0.5 hours, and then cooled to 40°C within 8 seconds to obtain a breeding medium, and added to four chemostats;
[0062] The strains of lactic acid bacteria, bifidobacteria, Bacillus subtilis and Candida utilis cultivated in the laboratory were respectively inserted into four chemos...
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