Process for producing fermented soybean meal through solid state fermentation of mixed culture

A technology for fermented soybean meal and solid-state fermentation, which is applied in the field of solid-state fermentation of mixed strains to produce fermented soybean meal, can solve the problems of heat-sensitive material loss, unfavorable large-scale production, and reduced product nutrition, so as to reduce operation and time, promote further proliferation, and ensure The effect of viable bacterial content

Inactive Publication Date: 2014-09-24
李晓叶
View PDF5 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drying of fermented soybean meal is a common problem. High-temperature drying will cause serious loss of heat-sensitive substances such as volatile sour substances in fermented soybean meal, reducing the nutrition of the product; low-temperature drying will increase the cost of fermentation, which is not conducive to large-scale production
[0003] Patent CN103315143A discloses a preparation method of low-temperature drying fermented soybean meal. Mixed strains ferment wet soybean meal and molasses, and then use low-temperature drying to treat the fermented soybean meal....

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] (1) Preparation of bacterial strain:

[0032] The following raw materials are added to the batching tank: barley malt, peptone, agar, sodium citrate, potassium dihydrogen phosphate, magnesium sulfate, sodium bicarbonate and water, and the mass ratio of the raw materials is barley malt: peptone: agar: citric acid Sodium: potassium dihydrogen phosphate: magnesium sulfate: sodium bicarbonate: water = 2.0-3.0: 1.5-1.8: 2.0-3.0: 0.5-1.0: 0.3-0.4: 0.015-0.020: 0.5-0.8: 180-220, heated to Boil, adjust the pH value to 4.5-5.5 and transfer it to the saccharification tank, add 9×10 to the saccharification tank 5 U-10×10 5 The saccharifying enzyme of U is kept at 55-65°C for 3-5 hours to obtain a saccharification solution, and the obtained saccharification solution is transferred into a fluid heater and heated to 95-100°C, incubated for 0.5 hours, and then cooled to 35°C in 5-10 seconds. -45°C, the breeding medium was obtained and added to the four chemostats;

[0033] The str...

Embodiment 1

[0051] This embodiment includes the following steps:

[0052] (1) Preparation of bacterial strain:

[0053] By following raw material: barley malt 2.5kg, peptone 1.7kg, agar 2.5kg, sodium citrate 0.8kg, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.018kg, sodium bicarbonate 0.7kg and water 190kg are added in the batching tank, heated To boiling, adjust the pH value to 4.8 and transfer to the saccharification tank, add 9×10 to the saccharification tank 5 The saccharifying enzyme of U was incubated for 3 hours at 65°C for saccharification to obtain a saccharification solution, and the obtained saccharification solution was transferred into a fluid heater and heated to 100°C, incubated for 0.5 hours, and then cooled to 40°C within 6 seconds to obtain a breeding medium, and added to four chemostats;

[0054] The strains of lactic acid bacteria, bifidobacteria, Bacillus subtilis and Candida utilis cultivated in the laboratory were respectively inserted into four chemo...

Embodiment 2

[0059] This embodiment includes the following steps:

[0060] (1) Preparation of bacterial strain:

[0061] The following raw materials: barley malt 2.4kg, peptone 1.6kg, agar 2.4kg, sodium citrate 0.8kg, potassium dihydrogen phosphate 0.36kg, magnesium sulfate 0.019kg, sodium bicarbonate 0.6kg and water 200kg are added to the batching tank, heated To boiling, adjust the pH value to 5.0 and transfer to the saccharification tank, add 9.6×10 to the saccharification tank 5 The saccharifying enzyme of U was incubated for 4 hours at 60°C for saccharification to obtain a saccharification solution, and the obtained saccharification solution was transferred into a fluid heater and heated to 95°C, kept for 0.5 hours, and then cooled to 40°C within 8 seconds to obtain a breeding medium, and added to four chemostats;

[0062] The strains of lactic acid bacteria, bifidobacteria, Bacillus subtilis and Candida utilis cultivated in the laboratory were respectively inserted into four chemos...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a process for producing fermented soybean meal through the solid state fermentation of mixed culture. A homemade breeding culture medium can be used for simultaneously breeding and culturing four kinds of cultures and then obtained culture solutions are used for fermenting the soybean meal; few breeding and fermentation procedures are adopted, the process is easy in operation and easy to achieve large-scale production; the fermented soybean meal is low in water content, loose, non-viscous and short in drying time, so that the production cost is lowered; in addition, the drying is performed at a relatively-low temperature, so that the biological activity of probiotics in the soybean meal is maintained. The process disclosed by the invention is used for fermentation production of the soybean meal by using mixed cultures of candida utilis, bacillus subtilis, lactobacillus and bifidobacterium; the fermented soybean meal contains a great number of probiotic groups, and a trypsin inhibitor is removed effectively; as an animal feed, the fermented soybean meal can be used for improving the immunity of animals and reducing the occurrence of diseases. Used as the animal feed, the fermented soybean meal produced by adopting the process disclosed by the invention is relatively easy to digest and absorb and can be used for replacing fish meal to be applied to aquaculture feeds and pig and chicken feeds.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a process for producing fermented soybean meal by solid-state fermentation of mixed strains. Background technique [0002] Soybean meal is a by-product obtained after soybean oil is extracted from soybean. It is the one with the largest output and the most widely used among 12 kinds of animal and vegetable oil meal feed products such as cottonseed meal, peanut meal and rapeseed meal. Main ingredient for livestock and poultry feed, widely used in poultry and aquaculture. Bio-fermentation of soybean meal can remove anti-nutritional factors in soybean meal, and the fermented soybean meal contains a large number of probiotic components, which has high biological activity, can improve the microbial balance of animal intestines, and supplement a large number of active probiotics in the intestines It can inhibit the growth of harmful bacteria such as Escherichia coli an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23K1/14A23K1/00A23K10/12A23K10/37
CPCY02P60/87
Inventor 李晓叶
Owner 李晓叶
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap