Culture medium composition for culturing chlorella

A composition and medium technology, applied in the direction of microorganism-based methods, microorganisms, single-cell algae, etc., can solve the problems of low absorption and utilization efficiency, decline in algal cell quality, low protein and fatty acid content, etc., to reduce cultivation costs, Promotes oil accumulation and increases oil content

Active Publication Date: 2012-05-23
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to CO 2 The solubility in the culture medium is limited, the absorption and utilization efficiency is low, and the growth rate of algae cells is limited. After large-scale, open ponds are generally used for stocking, but it is easy to pollute and has disadvantages such as low cell density and large floor area.
The second is the heterotrophic culture method, which is to provide artificial carbon sources, such as glucose, etc. during the cu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Add 100mL of the medium shown below in a 250mL shake flask, the inoculation ratio is 10% (that is, the volume of the seed solution accounts for 10% of the culture volume), wherein the seeds are domesticated Chlorella, heterotrophic culture (filling volume 100mL , temperature 30 degrees, shaking table speed 100rpm) for 48 hours, the result showed that the maximum cell density was 14.24g / L, and the oil content was 30.8% (the total oil content in the cell accounts for the percentage of the total dry cell weight).

[0036] Per liter medium composition:

[0037] Glucose 15g Na 2 SO 3 0.040g

[0038] KNO 3 0.35g trace element mother liquor 0.5mL

[0039] K 2 HPO 4 ·3H 2 O 0.075g MgSO 4 ·7H 2 O 0.075g

[0040] CaCl 2 2H 2 O 0.025g KH 2 PO 4 0.175g

[0041] NaCl 0.025 Soil extract (soil extract) 30mL

[0042] FeCl 3 ·6H 2 O 0.005g Fe-EDTA 1mL

[0043] Distilled water balance

[0044] Among them, the formula of 1 liter trace element mother solution: H 3 B...

Embodiment 2

[0049] Add 100mL of the medium shown below in a 250mL shake flask, and the inoculation ratio is 10% (that is, the volume of the seed solution accounts for 10% of the culture volume). Amount of 100mL, temperature 30 degrees, shaking table speed 100rpm) 48 hours, the maximum cell density measured as a result was 19.41g / L, and the oil content was 35.2% (the total oil content in the cell accounts for the percentage of the total cell dry weight).

[0050] The fungus residue treatment solution is obtained by hydrolyzing the bacteria or yeast residue. Among them, Klebsiella pneumoniae is preferred, which uses glycerol as the only substrate to ferment and produce 1,3-propanediol under anaerobic conditions. After fermentation, after cooking at 80°C for 40 minutes, filter the filter cake of bacterial residue at room temperature, dry the filter cake of bacterial residue, and then hydrolyze it in 0.1mol / L NaOH solution. The solid-to-liquid ratio of the hydrolysis process is 1:20, the tre...

Embodiment 3

[0065] Add 100mL of the medium shown below to a 250mL shake flask, and the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume). The seeds used are from Chlorella vulgaris, which has not been cultured heterotrophically process, directly to its heterotrophic culture (filling volume 100mL, temperature 30 degrees, shaker rotation speed 100rpm) 48 hours, the result records that the maximum cell density is 11.30g / L, and the oil content is 28.6% (the total oil content in the cell accounts for percentage of total cell dry weight).

[0066] The composition of each liter medium is as follows:

[0067] Glucose 30g Na 2 SO 3 0.020g

[0068] KNO 3 0.25g trace element mother liquor 0.1mL

[0069] K 2 HPO 4 ·3H 2 O 0.075g MgSO 4 ·7H 2 O 0.075g

[0070] CaCl 2 2H 2 O 0.025g KH 2 PO 4 0.175g

[0071] NaCl 0.025 Soil extract (soil extract) 30mL

[0072] FeCl 3 ·6H 2 O 0.005g Fe-EDTA 1mL

[0073] Distilled water balance ...

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Abstract

The invention relates to a culture medium composition suitable for culturing ordinary chlorella and a method for mixed culture of the chlorella by using the culture medium. The culture medium is obtained by adding 0.00126-0.063 g/L of Na2SO3 and an organic carbon source based on a common SE culture medium. The use of the culture medium in photoautotrophy-heterotrophy mixed culture of the ordinarychlorella can remarkably promote the growth of chlorella, and can allow the cell density of the ordinary chlorella to reach a level of high density culture in a short culture period, that is, the high density culture of the chlorella is realized. The use of the culture medium composition allows the ordinary chlorella to easily use the organic carbon source, especially a cheap carbon source for growing, can promote the later grease accumulation, and can greatly increase the quality of the ordinary chlorella, i.e. the grease content of the ordinary chlorella compared with heterotrophic culture to reach the level of photoautotrophic culture.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a medium composition suitable for high-density and high-quality cultivation of chlorella vulgaris and a method for cultivating chlorella. Background technique [0002] People have a long history of research on chlorella, among which Chlorella vulgaris is a species of algae that has been studied more, and it is also one of the species of algae commonly used in commercial production. [0003] Chlorella is rich in protein, polysaccharides, unsaturated fatty acids and other nutrients, which can be used in food, medicine and energy; it can accumulate fatty acids in large quantities, and the fatty acid content of some chlorella can account for 30% to 60% of dry weight. Utilizing the cultivation of chlorella to accumulate oil resources has become the most popular research field in the development of renewable resources using solar energy. Not only has strong market potential, b...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 王领民金平师文静李晓姝王崇辉佟明友黎元生
Owner CHINA PETROLEUM & CHEM CORP
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