Simple storage method for potato late blight bacteria

Abstract

The invention discloses a simple storage method for potato late blight bacteria. The method comprises the following steps: taking sterile water as a storage carrier for storing the potato late blightbacteria, specifically, pouring a solid medium into a culture dish, inoculating the potato late blight bacteria into the center of the solid medium, causing the potato late blight bacteria to grow andmultiply at 17 DEG C, transferring the grown potato late blight bacteria blocks into a triangular flask filled with sterile water and storing under 17 DEG C in a dark and sealed container, thereby realizing the simple, quick, medium and long term storage for the potato late blight bacteria. The storage method disclosed by the invention is characterized by simple operation, convenience, high speedand low cost; the treated potato late blight bacteria are long in storage period and stable in culture characters; the regular transplanting in the storage period is not required; the storage cost and space are saved, the storage is convenient and quick and the required culture can be stored at any time; and a basic guarantee is supplied for the biological characteristics of the potato late blight bacteria, the breeding for disease resistance of the potato and the research on diseases.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and in particular relates to a simple preservation method for potato infestans. Background technique [0002] Potatoes are perennial herbaceous plants belonging to the family Solanaceae. The tubers are edible and are the fourth most important food crop in the world. The main producing countries are China, Russia, India, Ukraine, the United States, etc. China is the country with the largest potato production in the world. Potato infestans (Phytophthora infestans) is the pathogen that causes late blight of potatoes and tomatoes, and the loss of potato yield caused by Phytophthora infestans exceeds 500 million US dollars every year. It occurs in all potato producing areas in China, and it is more serious in the southwest region, and it is more serious in rainy and humid years in the northeast, north China and northwest China. Potato late blight can damage potato leaves, petioles, stems and tub...

AI Technical Summary

Problems solved by technology

It is difficult to preserve the strain in vitro for a long time

Commonly used preservation methods in the laboratory, such as the culture medium slant preservation method, place the strains to be preserved in a 4°C refrigerator after the culture medium is overgrown; this preservation method has a short preservation time and requires regular transplantation, which not only increases the probability of contamination , and consume a lot of manpower and material resources

Freeze vacuum drying and cryopreservation require special equipment and high storage costs

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