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Application of a kind of isoflavone compound for preparing agricultural fungicide

A technology for agricultural fungicides and compounds is applied in the field of preparing agricultural fungicides to achieve the effects of excellent bacteriostatic effect, wide application prospects and environmental safety.

Active Publication Date: 2017-11-07
四川明峰洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are no relevant reports on the bactericidal activity of this compound against agricultural fungi and bacterial diseases

Method used

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  • Application of a kind of isoflavone compound for preparing agricultural fungicide
  • Application of a kind of isoflavone compound for preparing agricultural fungicide
  • Application of a kind of isoflavone compound for preparing agricultural fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Antibacterial activity of Glycyrrhizin of formula (I) of the present invention

[0024] The microdilution method was used to determine the effects of Glycyrrhizin on ginger blast fungus (R.solanacearum), tomato bacterial wilt (P.solanacearum), cabbage soft rot fungus (E.carotovora), and kiwi fruit canker (P.syringaepv. The antibacterial activity of the tested bacteria. The strains are provided by the Harmless Pesticide Research Laboratory of Sichuan Agricultural University.

[0025] The test bacteria were connected to Mueller-Hinton broth medium and cultured at 35°C for 48 hours. After reaching a slight turbidity, they were transferred to 0.9% saline and adjusted to Mai's turbidity 0.5 (the measurement method is to use a spectrophotometer to make it The absorbance is 0.020 at a wavelength of 650nm), and then diluted 200 times with Mueller-Hinton broth medium for use. Connect the diluted bacterial solution to a 96-well plate, add 100μl to each well, and then add 100μl of th...

Embodiment 2

[0030] The effects of Glycyrrhizin on Botrytis cinerea, Fusarium graminearum, Phytophthora infestans, Pyriculariagrisea, and Colletotrichum (Colletotrichum) gloeosporioides) and other 5 test fungi. The strains are provided by the Harmless Pesticide Research Laboratory of Sichuan Agricultural University.

[0031] Inhibition of spore germination: the spores of pathogenic fungi are mixed into a certain concentration of spore suspension (20-40 spores in each field of view under a 10×10 low power lens), and different concentrations of glycyrrhizin are combined with the spore suspension After mixing, drop the drops on the concave glass slide, and repeat the treatment 3 times. Carbendazim is used as the control of the drug, and sterile water is used as the blank control. After moisturizing culture at 25℃ for 8h-12h, check the germination of control spores. When the control germination rate reached 90%, check the germination rate of all treatments. The spore germ tube length is greate...

Embodiment 3

[0036] Example 3 Combination of Glycyrrhizin and Levo-Shikonin on Pathogenic Fungi

[0037] 1.1 Inhibitory toxicity of Glycyrrhizin and Levo-Shikonin to pathogenic fungi

[0038] The virulence of Glycyrrhizin and Levo-shikonin to Fusarium graminearum and Colletotrichum gloeosporioides was determined with reference to the method in Example 2.

[0039] 1.2 Study on the compound effect of two compounds

[0040] Combine Glycyrrhizin and Levo-Shikonin: The two compounds are formulated into 5 ratios (mass ratio) according to the mass ratio of 3:1, 2:1, 1:1, 1:2, and 1:3 . The above formulas were tested for toxicity according to the method of drug toxicity determination.

[0041] To evaluate the combined effect of the mixture, with EC 50 Based on the value, the combined effect type of the mixture is evaluated by calculating the co-toxicity coefficient of the mixture.

[0042]

[0043] CTC≥120 is synergistic effect, 80≤CTC <120 is additive, CTC <80 is the antagonistic effect.

[0044] 1.3 Test ...

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Abstract

The present invention relates to the use of an isoflavone compound, glabridin, in the preparation of an agricultural fungicide, and the compound is used to inhibit the following crop pathogenic fungi: Fusarium graminearum, Colletotrichum gloeosporioides, Strawberry gray mold (Botrytis cinerea), potato late blight (Phytophthora infestans) or rice blast fungus (Pyricularia grisea), and the following crop pathogenic bacteria: Chinese cabbage soft rot (Erwina carotovora), tomato bacterial wilt (Pseudomonas solanacearum ), ginger blast fungus (Ralstonia solanacearum) or kiwi fruit canker fungus (Pseudomonas syringae pv.Actinidae); in addition, the compound can also be made into various dosage forms with other pesticide adjuvants, and the compound and levoshikonin can be prepared in a certain amount The weight ratio has a synergistic effect when used in combination.

Description

Technical field [0001] The invention belongs to the field of agricultural fungicides, and specifically relates to the use of glycyrrhizin for preparing agricultural fungicides. technical background [0002] Glabridin (Glabridin) is a isoflavone compound, which is mainly isolated from Glycyrrhiza glabra. At present, the use of Glycyrrhizin is mainly concentrated in the field of medicine, and it has shown greater applications in anti-oxidation, anti-inflammatory, anti-atherosclerosis, lowering blood lipids, lowering blood pressure, neuroprotection, memory enhancement, and anti-obesity. Value, among which the most researched are the effects of antioxidant and anti-inflammatory. For example, research by Vaya (1997) et al. found that Glycyrrhizin can inhibit the consumption of β-carotene and at the same time affect the formation of CLOOH and the consumption of CL in the LDL oxidation reaction induced by AAPH. Kent (2002) et al. found that Glycyrrhizin exhibits a significant anti-rad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P3/00A01P1/00
Inventor 杨春平陈华保钱勇张敏龚国淑常小丽蒋春先王海建李沛利邱小燕田悦
Owner 四川明峰洋生物科技有限公司
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