75 results about "Dipicolinic acid" patented technology

The invention relates to methods and kits and methods for assessing the effectiveness of a sterilization process by determining the release of dipicolinic acid (DPA) from bacterial or other spores that contain DPA. A biological indicator containing a spore may be included together with articles being sterilized, and an assay of DPA released from the spore can be performed moments after the sterilization process is completed, or during the process. The kits and methods thus provide a rapid and reliable method of assessing the effectiveness of a sterilization process and, consequently, assure the sterility of article subjected to the same process.

The invention provides a dual-organic-ligand MOF, the MOF structurally comprises two different organic ligands with dicarboxylic structures; and the two different organic ligands with the dicarboxylicstructures comprise a phthalic acid organic ligand and a dipicolinic acid organic ligand. The structure of the provided dual-organic-ligand MOF comprises two different organic ligands, different organic ligands in the framework have different chemical functional groups, a chemical formula having an amino structure and a pyridine structure is obtained, the framework structure is charged due to thetwo organic ligands with two different chemical functional groups, the limit of the neutral framework of the MOF is broken, in addition to the fact that the positively charged framework structure with a high specific surface area shows the characteristic of the high specific surface area in the adsorption process, the MOF having different functional groups and the charged framework structure strengthens the adsorption effect in the dye wastewater, and has a better effect on adsorbing charged substances like an anionic dye.

The invention discloses a rate-type rare-earth fluorescent probe Tb/DPA@SiO2-Eu/GMP, a preparation method of visual test paper and an application thereof in the aspect of detecting bacillus-anthracis biomarker, namely 2, 6 dipicolinic acid (DPA). After the fluorescent probe is synthesized by a reverse microemulsion method, the quantitative detection for the concentration of the DPA is realized by utilizing a time-resolved fluorescence analysis method. Further, the portable visual test paper is prepared by soaking filter paper in a fluorescent probe solution, and can be applied to mobile-phone software for recognizing colors to detect the concentration of the DPA. The invention has the advantages that the currently-common traditional detection mode is changed, the complex operation process is not needed and the cost is saved. The invention has the characteristics of high accuracy, sensitivity and selectivity. The prepared test paper has the advantages of being convenient in carry and use, low in price and the like and has wide application prospect.

A lanthanide is combined with a medium to be tested for endospores. The dipicolinic acid released from the endospores binds the lanthanides, which have distinctive emission (i.e., luminescence) spectra, and are detected using photoluminescence. The concentration of spores is determined by preparing a calibration curve generated from photoluminescence spectra of lanthanide complex mixed with spores of a known concentration. A lanthanide complex is used as the analysis reagent, and is comprised of lanthanide ions bound to multidentate ligands that increase the dipicolinic acid binding constant through a cooperative binding effect with respect to lanthanide chloride. The resulting combined effect of increasing the binding constant and eliminating coordinated water and multiple equilibria increase the sensitivity of the endospore assay by an estimated three to four orders of magnitude over prior art of endospore detection based on lanthanide luminescence.

The invention relates to micro-electronic multilayer metal film etching liquid and application thereof. The micro-electronic multilayer metal film etching liquid comprises, by weight percentage, 5%-25% of hydrogen peroxide, 3%-10% of auxiliary acid, 2%-10% of a hydrogen peroxide stabilizer, 0.001%-1% of a taper angle control agent, 0.1%-1% of an additive and the balance ultrapure water. The auxiliary acid is an acidic material capable of providing H+; the hydrogen peroxide stabilizer is a combination of three kinds of bis-phosphonic acid tetrasodium, 8-hydroxyquinoline, citric acid, dipicolinic acid, aminoethanol phosphoric acid, picolinic acid and polyacrylamide; and the taper angle control agent is an organic compound containing amidogens, and the carbon chain length of the taper angle control agent ranges from 1 to 10. According to the micro-electronic multilayer metal film etching liquid, the special hydrogen peroxide stabilizer is selected, the additive in the etching liquid is used in a matched manner, and therefore the long-period stored etching liquid can be obtained; and meanwhile, the etching capacity of the etching liquid is stabilized.

The invention discloses a method for preparing a moxifloxacin hydrochloride intermediate, which comprises the following steps of: adding methylamine and 2,3-dipicolinic acid into a reaction vessel to perform a reflux reaction until the 2,3-dipicolinic acid is reacted completely, and carrying out post treatment to obtain an intermediate A; dissolving the intermediate A into alcohol, adding Pd-C with stirring, carrying out catalytic hydrogenation in a high pressure kettle with a pressure of 3Mpa and a temperature of 65 DEG C until the intermediate A is reacted completely, and carrying out post treatment to obtain an intermediate B; and adding tetrahydrofuran into the reaction vessel, carrying out ice-bath cooling to a temperature of below 5 DEG C, adding sodium borohydride and boron fluoride etherate, controlling a temperature in a range of below 15 DEG C, adding the intermediate B, performing a reflux reaction until the intermediate B is reacted to be disappeared, and carrying out post treatment to obtain an intermediate C. The method for preparing the moxifloxacin hydrochloride intermediate, which is provided by the invention, is simple to operate and has few steps and high yield, and the industrial production is easy to realize.

The invention discloses a synthesis method for a moxifloxacin side chain.With 2,3-dipicolinic acid being a raw material, cyclization, catalytic hydrogenation, resolution and racemization are performed, and then chemical reduction and debenzylation are performed to obtain moxifloxacin side chain.Resolution, racemization and chemical reduction are performed in sequence, sodium borohydride is used for replacing high-risk lithium aluminum hydride to synthesize the moxifloxacin side chain, and the synthesis method has the advantages that industrial waste materials are reduced, the production cost is lowered, and productivity is increased.

The invention provides a method for recycling rare earth ions by using bacillus subtilis spores, and belongs to the technical field of recycling of mineral resources. The method for recycling the rareearth ions by using he bacillus subtilis spores comprises the following steps of (1) preparation of the spores; (2) collection of the spores; (3) adsorption of the spores; and (4) elution of the spores and recycling of the rare earth ions. The spores formed by using bacillus subtilis when nutritional conditions are bad has high adsorbability for the rare earth ions, the adsorption effect is obviously superior to a bacillus subtilis body, the spores have excellent adsorbability, elution can be realized by adding excessive 2,6-dipicolinic acids under the neutral condition after the adsorption,the elution of the spores and recycling of the rare earth ions are realized, the recycling process is simple, convenient and rapid, the spores after the elution can be reused, and resource waste and environmental pollution are reduced.

The invention discloses a chromium ion colorimetric detection probe, namely application of 2,6-dipicolinic acid functionalized gold nano-particles in detection of chromium ions. The chromium ions are Cr<3+> or Cr<6+>. The invention provides a method for preparing the 2,6-dipicolinic acid functionalized gold nano-particles of the claim 1. The method is characterized by comprising the following steps: adding 10mL of a 0.5mM chloroauric acid water solution into a bottle; rapidly adding 4mL of a 2.5*10<-3>M to 1.25*10<-3>M 2,6-dipicolinic acid water solution under violent stirring; agitating for two minutes and adding 1.2mL of a 0.01M NaBH4 solution; agitating a mixed solution to react for one hour until the color of the solution is changed into red from no color; and stopping agitating to obtain the solution, namely the 2,6-dipicolinic acid functionalized gold nano-particles. The chromium ion colorimetric detection probe has the beneficial effects of high sensitivity, good selectivity and low detection limit.

The invention relates to a lithium-ion battery anode material and discloses a preparation method for the lithium-ion battery anode material. The preparation method I is using dipicolinic acid and LiOH.H2O to prepare pyridinium lithium carboxylate through a reflow reaction method; the preparation method II is using dipicolinic acid and inorganic salt containing Li<+> to prepare pyridinium lithium dicarboxylate in a reaction kettle through a hydrothermal method. The preparation method has a simple process, is convenient and easy to operate, and is extensive in material source.

The invention discloses a preparation method of a carbon-nitrogen-based iron cobalt oxide nanocluster MOF catalyst and application of the catalyst in electrocatalytic nitrogen fixation, and belongs tothe fields of nanomaterials, catalytic technologies and the like. The preparation method comprises the following main steps: taking 2,6-dipicolinic acid, iron nitrate and cobalt nitrate as raw materials, and blending at room temperature to prepare FeCo-MOF nanoclusters; heating the FeCo-MOF nanoclusters in air atmosphere to obtain the carbon-nitrogen-based iron cobalt oxide nanocluster MOF catalyst. The raw materials for preparing the catalyst are low in cost; the preparation method has the advantages of simple preparation process, low reaction energy consumption and industrial application prospect. The catalyst is used for high-efficiency electrocatalytic nitrogen fixation and has good electrochemical nitrogen fixation activity.

The invention discloses a fluorescent probe for detecting the concentration of 2,6-dipicolinic acid with a fluorescent method. The fluorescent probe comprises gold nano-cluster particles doped with europium elements, and the particle size of the fluorescent probe is 1 to 2nm. Through combination of the luminescence of rare-earth europium elements and the stable luminescence of gold nano-clusters,the obtained europium-doped gold nano-cluster fluorescent probe has the characteristic of ratio-dependent luminescence, so that the environmental built-in interference is eliminated; a stable luminescence signal, high detection accuracy, high sensitivity and a wide detection range are achieved; and the detection limit is lowered. The invention also provides a biosensor for detecting the concentration of 2,6-dipicolinic acid with the fluorescent method. Through combination of the europium-doped gold nano-cluster fluorescent probe and a standard curve, subsequent detection steps can be simplified; high sensitivity, high accuracy and a wide detection range are achieved; and other precise and precious instruments are not required. The invention also discloses preparation methods of the fluorescent probe and the biosensor. The preparation methods have the advantages of simple preparation process, easiness in operation, low cost and suitability for popularization and use.

The invention discloses a metal organic complex having antineoplastic activity. A chemical reaction of the complex is [Pr(C7H3NO4)3].C4H10N2, the molecular weight is 1677.01, is characterized by comprising a zero-dimensional structure and a trigonal system, a space group is P-3, the unit cell parameter is a=14.953(2), b=14.953(2), c=10.099(2), alpha=beta=90 DEG, gamma=120 DEG, V=1955.5(5), Z=1; wherein the metal organic complex is prepared by taking Pr(NO3)3.6H2O, 2,6-dipicolinic acid, and anhydrous piperazine as raw materials through a hydrothermal method. The process is simple, cost is low, the obtained complex has good inhibition effect on chronic granulocytic leukemia cell strain K562 and esophageal cancer cell line OE-19, and is hopeful for preparing the corresponding antitumor drug.

The invention discloses a method for synthesizing 2,6-dipicolinic acid. The method comprises the following steps: under the condition of air isolation, by using anhydrous diethyl ether or THF (tetrahydrofuran) as solvent, reacting 2,6-dichloropyridine used as a raw material with active metal under the action of an initiator to obtain a Grignard reagent; and cooling, then introducing excessive dry carbon dioxide gas, and acidifying to obtain the 2,6-dipicolinic acid. According to the invention, the cheap 2,6-dichloropyridine is used as the raw material, so the cost is low; and the synthesis process route is simple and easy to control, and can easily implement industrial production.

The invention discloses a synthetic method for 2,3-dipicolinic acid. According to the invention, CuSO4.5H2O, sulfuric acid, sodium quinoline chlorate, methyl 4-formylbenzoate, pyrrole, H2TCPP-OMe andMnCl2.6H2O are used as raw materials. The synthetic method of the invention uses quinoline for oxidative ring-opening in the oxidation system of NaClO3-H2SO4-CuSO4 under the action of the catalyst Mn-TCPP and then successively carries out alkali hydrolysis and acidification to obtain 2,3-dipicolinic acid. As the phenyl ring and the double bond of the catalyst Mn-TCPP are connected with electron-donating groups, electron cloud density on the double bond can be increased, and it is easier to attack and activate reactant intermediates; so ring-opening oxidation effect is improved, and yield is significantly higher than the yield of products obtained by conventional oxidation of potassium permanganate.

The invention relates to the field of fine chemical synthesis, in particular to a preparation method of 2, 3-dipicolinic acid. According to the method, oxygen is adopted to oxidize quinoline under the reaction pressure of 2-4 MPa in the presence of sulfuric acid and copper sulfate, the finished product of 2, 3-dipicolinic acid is obtained through filtration, alkali fusion, decoloration, acid pickling and drying, waste liquid generated in the reaction can be directly applied to the production process of 2, 3-dipicolinic acid, and the yield is increased to 83% or above. According to the method, the magnetic activated carbon prepared from the cotton straw is adopted as the adsorbent to perform decoloration and impurity removal on the 2, 3-dipicolinic acid crude product, the adsorption effect is good, the 2, 3-dipicolinic acid product is a white crystal, and the product purity is as high as 99.97%.

The invention relates to the field of pesticide chemistry, in particular to a preparation method of a 2, 3-dipicolinic acid ester derivative intermediate and a 2, 3-dipicolinic acid ester derivative, which comprises the following steps: dissolving a general formula II and a general formula III in an organic solvent, adding a strong base for reaction, filtering, washing, and drying to obtain the 2, 3-dipicolinic acid ester derivative intermediate. After the reaction is finished, carrying out acidification treatment by using an alcoholic solution of anhydrous acid to obtain an acidification reaction solution of the intermediate I; carrying out heat treatment on an acidification reaction solution of the intermediate I, then dropwise adding a general formula IV, and after the reaction is finished, carrying out simple post-treatment to obtain a general formula V; according to the method for preparing the 2, 3-dipicolinic acid ester and the derivative thereof, the two-step reaction can be carried out through a one-pot boiling method, free ammonia generated in the second step provides an ammonia source for pyridine ring formation, and the whole process is safe, environmentally friendly, high in atom utilization rate, economical, efficient and suitable for industrial production.