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Sporicidal methods and compositions

A composition and spore technology, applied in the direction of organic/inorganic compound composition, botanical equipment and method, biocide, etc., can solve the problems such as difficult to kill endospores

Pending Publication Date: 2021-02-19
EVONIK OPERATIONS GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, standard antimicrobial treatments do not readily kill endospores

Method used

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  • Sporicidal methods and compositions
  • Sporicidal methods and compositions
  • Sporicidal methods and compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] microorganism All microorganisms were purchased as spores from certified producers. The microorganisms and manufacturers are listed below.

[0042] Paenibacillus chiba-American Type Culture Collection (ATCC);

[0043] Paenibacillus favisporus-DSMZ;

[0044] Bacillus cereus 14579 – Presque Isle Cultures;

[0045] Bacillus atrophaeus 9372 – Mesa Labs;

[0046] Bacillus subtilis 6633 – Presque Isle Cultures;

[0047] Bacillus subtilis 19659 – Presque Isle Cultures.

[0048] Upon receipt, by serial dilution in Butterfield buffer and then in 3MAC Petrifilm TM The plate was plated and the titer of the spore culture was determined. Then Petrifilm TM Incubate at 35°C for about 48 hours and count. Store the culture in a dedicated refrigerator. Before each use, re-titre the spore culture to confirm that the culture is still viable and not contaminated.

Embodiment 2

[0050] With PAA / H shown in Table 1 2 o 2 Ratio of PAA formulations to treat Paenibacillus achiba BAA-725.

[0051] Table 1: PAA / H 2 o 2 Preparation

[0052] Preparation 1 2 3 4 5 6 7 Nominal PAA, wt% 5 15 15 22 35 15 22 Nominal H 2 o 2 ,weight%

23 23 10 10 7 3 4 PAA / H 2 o 2 Compare

0.2 0.6 1.5 2.2 5.0 5.0 5.0

[0053] The PAA formulation was diluted to about 2900 ppm in deionized water before use. The temperature of the formulation was raised to 55 °C and the actual PAA and H were measured in an automatic titrator just before use. 2 o 2 concentration.

[0054] Paenibacillus chiliba BAA-725 was inoculated onto stainless steel bars. Exposure to Paenibacillus chiliba BAA-725 was performed by dipping the bar into the PAA formulation for 10 seconds, 20 seconds or 30 seconds. All viable microorganisms were recovered, cultured and counted. Microorganisms were recovered by suspending the culture in Letheen ...

Embodiment 3

[0058] In the presence and absence of catalase (a hydrolyzed H 2 o 2 In the case of the enzyme), Paenibacillus chiliba BAA-725 was treated with the PAA formulation.

[0059] PAA / H with deionized (DI) water 2 o 2 Equilibrium solution (with 35% by weight of PAA and 7% by weight of H 2 o 2 ) was diluted to a PAA concentration of approximately 2900ppm. For catalase treatment, add 1 μl of catalase (Sigma-Aldrich, catalase from Aspergillus, ≥4000 units / mg protein, about 14.4 mg / ml protein) to 200 ml solution, and Incubate at temperature for 45 minutes. The solution was then warmed to 55°C in a water bath. The heating step took about 38 minutes. After reaching the target temperature, measure the actual PAA and H with an automatic titrator 2 o 2 concentration.

[0060] According to the method described in Example 1, stainless steel bars were inoculated with Paenibacillus chiliba BAA-725 and contacted with catalase-digested PAA solution and non-catalase-digested control PAA ...

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Abstract

Provided herein are methods and compositions for the reduction of endospore contamination of a substrate with percarboxylic acid-based compositions. The methods can include the step of continuously monitoring the oxidation-reduction potential of the percarboxylic acid-based compositions.

Description

[0001] Cross References to Related Applications [0002] Pursuant to 35 U.S.C. §119§119(e)(1), this application claims priority to U.S. Provisional Application Serial No. 62 / 678,562, filed May 31, 2018, the contents of which are incorporated herein by reference. technical field [0003] The present invention relates to percarboxylic acid based compositions for reducing endospore contamination. Background technique [0004] Certain species of bacteria can form endospores under harsh conditions or nutrient deficiencies. Endospores are dormant, multilayered, nonreproductive structures that contain highly dense microbial DNA. Endospores are resistant to environmental damage, such as heat, UV radiation, desiccation, chemical and enzymatic exposure, which would normally kill bacteria. Therefore, standard antimicrobial treatments do not readily kill endospores. After exposure to appropriate conditions such as heat and nutrient media, endospores can germinate to produce live bact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/00A01N59/00A01N25/34
CPCA61L2/0088A61L2/186A61L2202/24A61L2202/26A01N59/00A61L2202/21A61L2202/23A01P1/00A01N37/16A01N37/00A01N25/34C11D3/39
Inventor W·安C·米蒂加R·米蒂加
Owner EVONIK OPERATIONS GMBH
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