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Preparation method of diosgenin chitosan carrier

A technology of diosgenin and transfer body, applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of low yield of diosgenin, cumbersome operation, and long consumption time, and achieve Shorten the separation time, optimize the preparation process, and have good anti-inflammatory effects

Active Publication Date: 2021-11-12
JIANGXI YONGTONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional acid hydrolysis method is cumbersome to operate, takes a long time, and has a low yield of diosgenin. Moreover, due to the large volume of medicinal materials, the consumption of acid is large, and the organic matter degraded by acid is large, resulting in a large amount of sewage and large environmental pollution, which seriously hinders Development of Diosgenin Industry
Using gasoline or petroleum ether to extract the hydrolyzate requires a large amount, and 120# gasoline is a first-class explosion-proof solvent with a high risk factor

Method used

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  • Preparation method of diosgenin chitosan carrier
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  • Preparation method of diosgenin chitosan carrier

Examples

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Effect test

Embodiment 1

[0026] A preparation method of diosgenin chitosan transfer body, which is extracted by the following steps:

[0027] S1. Crush 10 g of the rhizome of Dioscorea scutellariae and pass through an 80-mesh sieve, add 5 times the volume of deionized water to soak for 1 hour, adjust the pH to 6.5 with sodium acetate-acetic acid buffer, then add enzymes to enzymatically hydrolyze at 40°C for 45 minutes, then heat at 90°C After the enzyme is inactivated, adjust the pH to neutral, add 5 mL of sodium lauryl sulfate aqueous solution and 2 mL of magnesium chloride solution, soak for 3 hours, then undergo ultrasonic treatment at 24KHz for 30 minutes, then centrifuge at 4000r / min for 15 minutes, take the supernatant, filter, and obtain Ultrasonic extraction solution;

[0028] S2, add 0.2 times the volume of sulfuric acid solution into the ultrasonic extraction solution, stir and hydrolyze at 105°C for 2 hours, neutralize to neutral with saturated sodium carbonate solution after hydrolysis, o...

Embodiment 2

[0038] A preparation method of diosgenin chitosan transfer body, which is extracted by the following steps:

[0039] S1, crush 12 g of the rhizome of Dioscorea scutellariae through an 80-mesh sieve, add 5.5 times the volume of deionized water to soak for 1.5 hours, adjust the pH to 6.5 with sodium acetate-acetic acid buffer solution, then add enzymes to enzymolyze at 40°C for 50 minutes, 90 After the enzyme is inactivated at ℃, adjust the pH to neutral, add 7 mL of sodium lauryl sulfate aqueous solution and 2.5 mL of magnesium chloride solution, soak for 3.5 hours, and then undergo ultrasonic treatment at 25KHz for 35 minutes, then centrifuge at 4500r / min for 18 minutes, and take the supernatant. Filter to obtain ultrasonic extract;

[0040] S2, adding 0.2 times the volume of sulfuric acid solution into the ultrasonic extraction solution, stirred and hydrolyzed at 105°C for 2.5h, neutralized to neutral with saturated sodium carbonate solution after hydrolysis to obtain diosgen...

Embodiment 3

[0049] A preparation method of diosgenin chitosan transfer body, which is extracted by the following steps:

[0050] S1. Crush 15g of the rhizome of Dioscorea scutellariae through a 100-mesh sieve, add 6 times the volume of deionized water to soak for 2 hours, adjust the pH to 6.5 with sodium acetate-acetate buffer, then add enzymes to enzymatically hydrolyze at 40°C for 55min, then heat at 90°C After the enzyme is inactivated, adjust the pH to neutral, add 10 mL of sodium lauryl sulfate aqueous solution and 3 mL of magnesium chloride solution, soak for 4 hours, then undergo ultrasonic treatment at 26KHz for 40 minutes, then centrifuge at 5000r / min for 20 minutes, take the supernatant, filter, and obtain Ultrasonic extraction solution;

[0051] S2, add 0.2 times the volume of sulfuric acid solution into the ultrasonic extraction solution, stir and hydrolyze at 105°C for 3 hours, neutralize to neutral with saturated sodium carbonate solution after hydrolysis, obtain diosgenin h...

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Abstract

The invention discloses a preparation method of a diosgenin chitosan carrier. The preparation method is characterized by comprising the following steps of: firstly, crushing rhizomes of dioscorea zingiberensis, fermenting the rhizomes with an enzyme, and performing ultrasonic extraction with lauryl sodium sulfate and magnesium chloride to obtain an ultrasonic extracting solution; and hydrolyzing the ultrasonic extracting solution with a small amount of sulfuric acid to obtain a diosgenin hydrolysate, separating out foam with a foam separator, flushing the foam with a phosphate buffer salt solution, enabling the foam to flow into a dry lipid membrane prepared from egg yolk lecithin and cholesterol to form a liposome suspension, finally performing ultrasonic treatment, performing microfiltration, and performing chitosan wrapping to obtain the diosgenin chitosan carrier. The diosgenin yield is high, the acid consumption is low, and the diosgenin chitosan carrier is high in encapsulation efficiency, good in stability, free of irritation to human skin, high in skin permeability and high in anti-inflammatory capacity, has an inhibiting effect on tyrosinase and is applicable to cosmetics.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to a preparation method of a diosgenin chitosan transfer body. Background technique [0002] Diosgenin, known as diosgenin in industry, is the ligand of diosgenin. It is connected to the sugar group through a glycoside bond at the C-3 position. It mainly exists in the rhizome of diosgenin in the form of diosgenin, and is closely connected with cellulose. bound in the cell wall. Diosgenin is an ideal precursor for the synthesis of various steroidal hormones and steroidal contraceptives. More than 60% of the steroidal hormones produced in the world are produced from it. At the same time, it also has the effects of hemolysis, blood fat reduction, anti-tumor, antibacterial and anti-inflammatory. There is a huge demand for diosgenin at home and abroad, so developing a new production process for extracting diosgenin has important value and broad market potential. [0003] At present, th...

Claims

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Application Information

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IPC IPC(8): A61K8/63A61K8/73A61Q19/00A61Q19/08A61P29/00
CPCA61K8/63A61K8/736A61Q19/00A61Q19/08A61P29/00A61K2800/805A61K2800/782
Inventor 周晖刘涛杨学宁王炳权
Owner JIANGXI YONGTONG TECH
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