Method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae

A technology of dioscin and diosgenin, which is applied in the field of fermenting and preparing diosgenin by using endophytic bacteria of dioscens scutellaria, can solve the problem that the acidic wastewater has not been fundamentally solved, shorten the fermentation period, overcome the small yield, and increase the yield Effect

Inactive Publication Date: 2020-06-02
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current research and application direction is mainly to use isolated strains to ferment or naturally ferment the underground stems of Dioscorea scutellaria, and then isolate diosgenin. Compared with the conventional acid hydrolysis method, although the effective components of Dioscorea scutellaria can be fully utilized, the extraction of saponin can be significantly improved rate, but the problem of producing a large amount of acid wastewater in the production process has not been fundamentally resolved

Method used

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  • Method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae
  • Method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae
  • Method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae

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Embodiment 1

[0032] This embodiment provides a method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae. This method of preparing diosgenin is different from the existing preparation method of separating and purifying diosgenin from yam. It uses the endophytic bacterium SYt1 of Dioscorea scutellaria as the starting strain and separates it from its fermentation broth through a specific fermentation process. . The endophytic bacterium SYt1 of Dioscorea scutellaria used in this example is Bacillus licheniformis Syb06.11.1, and the strain was preserved in CGMCC on January 10, 2008, with a preservation number of 2334. See patent ZL200810150274.3 for the isolation and identification of this strain and the isolation of its metabolite diosgenin.

[0033] The method for fermenting and preparing diosgenin described in this example includes the following steps.

[0034] The first step is to expand the culture of endophyte SYt1 from Dioscorea scutellaria.

[0035...

Embodiment 2

[0046] In this example, a basic process for producing saponin by fermentation in a 5L small tank was established, that is, a fermentation tank with a volume of 5L was used to ferment the endophytic bacteria of Dioscorea scutellariae. In this example, the above-mentioned fermentation conditions were optimized, and the technological conditions for producing diosgenin by fermentation were sought.

[0047] (1) Temperature control

[0048] figure 1 The trend chart of the yield change of diosgenin in the fermentation broth under different fermentation temperatures is given. Depend on figure 1 It can be seen that the fermentation temperature is set to 24°C, 26°C, 28°C, 30°C and 32°C respectively, and the small-scale fermentation tank (5L) of the endophyte SYt1 of Dioscorea scutellaria is carried out in batches, and the objective function is established with the maximum diosgenin yield, An optimal temperature trajectory with the fermentation time was obtained by calculation. On th...

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Abstract

The invention belongs to the technical field of a microorganism secondary metabolite, and discloses a method for preparing diosgenine by a fermenting dioscorea zingiberensis endophyte. Diosgenine is obtained and separated from fermentation liquor of a dioscorea zingiberensis endophyte. The method comprises the following steps: 1) inoculating a strain culture medium with the dioscorea zingiberensis endophyte for strain culture to obtain a fermentation strain; 2) inoculating a fermentation tank containing a fermentation culture medium with the fermentation strain, starting stirring, and setting fermentation conditions of the fermentation temperature being 24-32 DEG C, the fermentation pH being 5.5-8.5, and the ventilation amount being 1.0-5.5 L / min; and 3) performing acidolysis to the obtained fermentation liquor, and carrying out neutralization to obtain diosgenine. According to a basic process, a 5L small tank is established for fermentation production of diosgenine. The yield of diosgenine is obviously increased, the technical defects of low yield of diosgenine and high energy consumption in the prior art are overcome, and research foundation is provided for development and utilization of dioscorea zingiberensis.

Description

technical field [0001] The invention belongs to the technical field of microbial secondary metabolites, and relates to the optimization of the preparation process of raw drug saponin, in particular to a method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae. Background technique [0002] Dioscorea zingiberensis C.H.Wright is a unique variety of dioscorea in my country, also known as turmeric, and is the main plant raw material for the production of saponin. Dioscin is called "medicinal gold" by the medical field, and it can synthesize thousands of steroid hormones such as corticosteroids, anabolic hormones, and sex hormones. Dioscorea shield leaf is one of the important raw materials for obtaining diosgenin. At present, 138 species of Dioscorea plants containing diosgenin have been found in the world, but only about 10% of the plants of the genus Dioscorea have industrial utilization value. With the continuous increase of diosgenin mar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/10
CPCC12P33/20
Inventor 秦宝福曹让吴养会郑立飞王建刚陈晓红胡仲秋任永茂崔向月向永生
Owner NORTHWEST A & F UNIV
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