Method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae
A technology of dioscin and diosgenin, which is applied in the field of fermenting and preparing diosgenin by using endophytic bacteria of dioscens scutellaria, can solve the problem that the acidic wastewater has not been fundamentally solved, shorten the fermentation period, overcome the small yield, and increase the yield Effect
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Embodiment 1
[0032] This embodiment provides a method for preparing diosgenin by fermenting endophytic bacteria from Dioscorea scutellariae. This method of preparing diosgenin is different from the existing preparation method of separating and purifying diosgenin from yam. It uses the endophytic bacterium SYt1 of Dioscorea scutellaria as the starting strain and separates it from its fermentation broth through a specific fermentation process. . The endophytic bacterium SYt1 of Dioscorea scutellaria used in this example is Bacillus licheniformis Syb06.11.1, and the strain was preserved in CGMCC on January 10, 2008, with a preservation number of 2334. See patent ZL200810150274.3 for the isolation and identification of this strain and the isolation of its metabolite diosgenin.
[0033] The method for fermenting and preparing diosgenin described in this example includes the following steps.
[0034] The first step is to expand the culture of endophyte SYt1 from Dioscorea scutellaria.
[0035...
Embodiment 2
[0046] In this example, a basic process for producing saponin by fermentation in a 5L small tank was established, that is, a fermentation tank with a volume of 5L was used to ferment the endophytic bacteria of Dioscorea scutellariae. In this example, the above-mentioned fermentation conditions were optimized, and the technological conditions for producing diosgenin by fermentation were sought.
[0047] (1) Temperature control
[0048] figure 1 The trend chart of the yield change of diosgenin in the fermentation broth under different fermentation temperatures is given. Depend on figure 1 It can be seen that the fermentation temperature is set to 24°C, 26°C, 28°C, 30°C and 32°C respectively, and the small-scale fermentation tank (5L) of the endophyte SYt1 of Dioscorea scutellaria is carried out in batches, and the objective function is established with the maximum diosgenin yield, An optimal temperature trajectory with the fermentation time was obtained by calculation. On th...
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