Screening method of endophyte of dioscorea zingiberensis for producing diosgenin

Abstract

The invention obtains an endophyte of a dioscorea zingiberensis by steps of field sampling, sample surface sterilization, isolation of microorganism, liquid culture and the like; the strain is identified as bacillus licheniformis, which can produce diosgenin of the dioscorea zingiberensis and can be used as production strain for fermentation.

Description

[0001] The invention relates to a production strain, which is isolated from Dioscorea scutellaria and can produce saponins. Background technique [0002] Dioscorea scutellaria is a medicinal plant. Traditional Chinese medicine believes that diosminum scutellaria is used as medicine with its rhizomes, and its nature and flavor are glycosides, bitter, and cool. It has the functions of dispelling dampness, clearing away heat and detoxifying. It is used in the folk to treat acute suppurative infection of the skin, soft tissue injury, bee stings, insect bites and various surgical inflammations. The rhizome also has the function of strengthening the body and bones. Studies in recent years have shown that yam has anti-inflammatory, antibacterial, anti-asthma, and hypolipidemic effects. Saponin is the main active ingredient of Dioscorea, and it is also an important precursor for the synthesis of various steroid hormones in my country. It can synthesize hundreds of steroid hormone drug...

AI Technical Summary

Problems solved by technology

With the increasing demand for sapogenin in the pharmaceutical industry, people have intensified the development of yam resources, which stimulated the high-speed and unrestrained use of wild yams, and the predatory excavation in many places has made wild resources gradually and if artificial planting is adopted, on the one hand, the cyclic planting-digging in the planting process of Dioscorea shield leaf will cause serious damage to the fragile ecological environment of its production area (mainly mountainous areas), especially cause the destruction of surface vegetation And cause water and soil erosion, on the other hand long-term artificial planting causes the degradation of yam germplasm resources, and its saponin content constantly reduces

At the same time, the production process of directly extracting sapogenin from yams will produce a large amount of wastewater, with an average of 450 tons of sapogenin produced per ton of sapogenin produced. These wastewaters have high concentrations of organic pollutants, high acidity, and high chroma, and are extremely difficult to treat.