Screening method of endophyte of dioscorea zingiberensis for producing diosgenin

A technology of Dioscorea scutellaria and endophytic bacteria, which is applied in the field of strain production, and can solve the problems of stimulating the high-speed and uncontrolled utilization of wild Dioscorea scutellaria scutellaria, ecological environment damage, and difficult treatment.

Inactive Publication Date: 2010-01-06
NORTHWEST A & F UNIV
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Problems solved by technology

With the increasing demand for sapogenin in the pharmaceutical industry, people have intensified the development of yam resources, which stimulated the high-speed and unrestrained use of wild yams, and the predatory excavation in many places has made wild resources gradually and if artificial planting is adopted, on the one hand, the cyclic planting-digging in the planting process of Dioscorea shield leaf will cause serious damage to the fragile ecological environment of its production area (mainly mountainous areas), especially cause the ...

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Embodiment Construction

[0017] The method of obtaining the endophytic bacteria of Dioscorea shield leaf is as follows:

[0018] a. collect samples of Dioscorea scutellaria from the wild;

[0019] b. Select the underground stems of the robust Dioscorea scutellariae, wash them with sterile water, put them into an ultra-clean workbench, cut them into small pieces of 5cm×5cm, soak them in 75% alcohol for 30s, and then soak them in 0.1% HgCl2 solution for 3- 5min, followed by rinsing 3 times with sterile water. And use two methods to test the surface sterilization effect respectively:

[0020] (1) Flushing solution method: Pipette the last sterile water rinsing solution on the potato dextrose agar medium plate, incubate in a constant temperature incubator at 27°C for 3-5 days, do 2 replicates for each material, and observe whether there are colonies produce.

[0021] (2) Raw material method: put the sterilized raw material on a potato dextrose agar medium plate, culture it in a constant temperature inc...

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Abstract

The invention obtains an endophyte of a dioscorea zingiberensis by steps of field sampling, sample surface sterilization, isolation of microorganism, liquid culture and the like; the strain is identified as bacillus licheniformis, which can produce diosgenin of the dioscorea zingiberensis and can be used as production strain for fermentation.

Description

[0001] The invention relates to a production strain, which is isolated from Dioscorea scutellaria and can produce saponins. Background technique [0002] Dioscorea scutellaria is a medicinal plant. Traditional Chinese medicine believes that diosminum scutellaria is used as medicine with its rhizomes, and its nature and flavor are glycosides, bitter, and cool. It has the functions of dispelling dampness, clearing away heat and detoxifying. It is used in the folk to treat acute suppurative infection of the skin, soft tissue injury, bee stings, insect bites and various surgical inflammations. The rhizome also has the function of strengthening the body and bones. Studies in recent years have shown that yam has anti-inflammatory, antibacterial, anti-asthma, and hypolipidemic effects. Saponin is the main active ingredient of Dioscorea, and it is also an important precursor for the synthesis of various steroid hormones in my country. It can synthesize hundreds of steroid hormone drug...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/16C12R1/10
Inventor 秦宝福李军超徐虹史鹏张继文
Owner NORTHWEST A & F UNIV
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