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Multifunctional microorganism soil repairing agent as well as preparation and application thereof

A technology of soil remediation agent and composite microbial agent, which is applied in the field of multifunctional microbial soil remediation agent and its preparation and application, can solve the problems of soil degradation, organic pollution, frequent occurrence of diseases and insect pests in agricultural environment, etc., and achieve wide remediation and low cost , the effect of preventing soil degradation

Inactive Publication Date: 2018-05-04
厦门天邦润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the deficiencies in the prior art, and provides a kind of multifunctional microbial soil remediation agent and preparation method thereof, adopts the multifunctional microbial soil remediation agent obtained by the present invention to be mainly used in solving pollutions such as heavy metal and petroleum hydrocarbon and PAHs in the soil environment and Organic pollution caused by agricultural chemical fertilizers and pesticides, and secondly, it can solve the problems of soil degradation and frequent occurrence of pests and diseases in the agricultural environment

Method used

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  • Multifunctional microorganism soil repairing agent as well as preparation and application thereof
  • Multifunctional microorganism soil repairing agent as well as preparation and application thereof
  • Multifunctional microorganism soil repairing agent as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 prepares multifunctional microbial soil remediation agent of the present invention according to the following steps:

[0034] (1) Take the following strains: Bacillus phenylevorus, Bacillus desertis Sonora, Bacillus sphaericus, Bacillus licheniformis, Bacillus circulans, Bacillus thuringiensis, Achromobacter denitrifying, and inoculate them in liquid seeds respectively The culture medium is liquid cultured at 32-37°C for 20-24h to obtain liquid seeds, then inoculate the liquid seeds in the liquid fermentation medium at an inoculum amount of 10%, and carry out liquid seed expansion fermentation culture at 32-37°C After 26-32 hours, dry and dehydrate to obtain the bacteria powders of the above-mentioned strains respectively, which are sequentially recorded as bacteria A, bacteria B, bacteria C, bacteria D, bacteria E, bacteria F, and bacteria G;

[0035] The number of viable bacteria contained in the bacterial powder of each strain is as follows: the number o...

Embodiment 2

[0047] Example 2 Take the composite microbial agent prepared according to the steps (1)-(6) of Example 1, and mix it with nano-chitosan, humic acid, and nano-biochar in the following weight ratio to prepare multifunctional microbial soil remediation Agent: 30 parts of nano-chitosan, 20 parts of humic acid, 20 parts of nano-biochar, 30 parts of compound microbial agent.

Embodiment 3

[0048] Example 3 Take the composite microbial agent prepared according to the steps (1)-(6) of Example 1, mix it with nano-chitosan, humic acid, and nano-biochar in the following weight ratio to prepare multifunctional microbial soil remediation Agent: 60 parts of nano-chitosan, 10 parts of humic acid, 10 parts of nano-biochar, 20 parts of compound microbial agent.

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Abstract

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.

Description

technical field [0001] The invention relates to a multifunctional microbial soil restoration agent and its preparation and application, belonging to the fields of biotechnology and environmental protection. Background technique [0002] At present, with the intensification of industrial and urban pollution and the increase in the types and quantities of agricultural chemicals, soil pollution has become a global environmental pollution issue that has attracted much attention. The area of ​​polluted cultivated land in China is about 150 million mu, of which organic pollution 36 million hm2 of farmland polluted by pollutants (pesticides, petroleum hydrocarbons and polycyclic aromatic hydrocarbons (PAHs)) 2 . Soil pollution is mainly caused by heavy metals and oil pollution caused by industrial activities and organic pollution caused by agricultural chemical fertilizers and pesticides. [0003] If soil pollution cannot be repaired in time, pollutants will inevitably be transfe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/07C12R1/10C12R1/09C12R1/01C12R1/365
CPCB09C1/10C12N1/20
Inventor 何圣平凌仕信
Owner 厦门天邦润生物科技有限公司
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