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Rapid propagation method and culture medium for Dioscorea composita tissue culture seedlings

A technology of Dioscorea chrysanthemum leaf and medium, applied in the field of plant tissue culture, can solve the problems of high cost, large demand for tubers, slow growth rate, etc.

Pending Publication Date: 2022-07-08
天津博奥聚能生物科技有限公司 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a dioecious Dioscorea chrysanthemum, the flowering period of female flowers and male flowers is different, and the seeds are not full enough, so it is only suitable for small-scale planting; tuber propagation requires a large demand for tubers, high cost, and slow growth rate

Method used

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  • Rapid propagation method and culture medium for Dioscorea composita tissue culture seedlings
  • Rapid propagation method and culture medium for Dioscorea composita tissue culture seedlings
  • Rapid propagation method and culture medium for Dioscorea composita tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] (1) The collected rhizome of Dioscorea japonica is simply washed with distilled water to remove impurities, and then the rhizome of Dioscorea japonica is planted in matrix soil (the matrix soil components are nutrient soil and vermiculite, and the nutrient soil and leech The mass ratio of the stone is 2:1) in the light intensity of 1500 lux, the light cycle is 15h / d, and the temperature is 25 ℃ for 8min, and the stronger vegetative organs (stems, leaves, and axillary buds) are grown. , terminal bud) after, choose stem as explant for subsequent use;

[0064] (2) the stem is sterilized by mercuric chloride of 0.05% for 5min by mass concentration, washed 5 times with sterile water, and blotted the surface moisture with sterile filter paper, spread on the dedifferentiation medium of Dioscorea chinensis tissue culture seedlings (the The dedifferentiation medium is based on the modified MS medium, including the following components: 6-BA 1 mg·L -1 , NAA 0.1mg·L -1 , sucrose...

Embodiment 2

[0069] (1) The collected rhizome of Dioscorea japonica is simply washed with distilled water to remove impurities, and then the rhizome of Dioscorea japonica is planted in matrix soil (the matrix soil components are nutrient soil and vermiculite, and the nutrient soil and leech The mass ratio of the stone is 3:1), the light intensity is 2000lux, the light cycle is 16h / d, and the temperature is 28°C for 10min, and the stronger vegetative organs (stems, leaves, and axillary buds) will grow. , terminal buds), select leaves as explants for subsequent use;

[0070] (2) the leaves are sterilized by mercuric chloride with a mass concentration of 0.1% for 10 min, washed 6 times with sterile water, and blotted the surface moisture with sterile filter paper, spread on the dedifferentiation medium of Dioscorea chinensis tissue culture seedlings (the The dedifferentiation medium is based on the modified MS medium, including the following components: 6-BA 2 mg L -1 , NAA 0.2mg·L -1 , suc...

Embodiment 3

[0075] (1) The collected rhizome of Dioscorea japonica is simply washed with distilled water to remove impurities, and then the rhizome of Dioscorea japonica is planted in matrix soil (the matrix soil components are nutrient soil and vermiculite, and the nutrient soil and leech The mass ratio of the stone is 4:1), the light intensity is 2500lux, the light cycle is 17h / d, and the temperature is 30°C for 12min, and the stronger vegetative organs (stems, leaves, and axillary buds) are to grow. , terminal bud) after, choose axillary bud as explant for subsequent use;

[0076] (2) the axillary buds were sterilized by mercuric chloride of 0.15% for 15min by mass concentration, washed 7 times with sterile water, and blotted the surface moisture with sterile filter paper, spread on the dedifferentiation medium of Dioscorea chinensis tissue culture seedlings (the The dedifferentiation medium is based on the modified MS medium, including the following components: 6-BA 3 mg L -1 , NAA 0...

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Abstract

The invention belongs to the technical field of plant tissue culture, and provides a rapid propagation method and a culture medium for Dioscorea composita tissue culture seedlings. According to the method, the axillary buds of dioscorea composita are used as explants, callus generation is promoted on an improved MS + 2mg / L 6-BA + 0.2 mg / L NAA culture medium, callus proliferation is promoted on an MS + 2mg / L 6-BA + 0.2 mg / L NAA + 0.05% glutamic acid culture medium, redifferentiation of cluster buds is promoted on MS + 2mg / L 6-BA + 0.4 mg / L NAA + 0.01% vitamin E + 0.025% selenocysteine, and complete root structures of the axillary buds are promoted on improved MS + 1mg / L IBA + 0.1 mg / L NAA. The invention provides a technical basis for factory scale breeding of the Dioscorea mexicana, and also lays a technical basis for genetic engineering improvement and molecular breeding of the Dioscorea mexicana.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a fast propagation method and a medium for tissue culture seedlings of Dioscorea japonica. Background technique [0002] Chrysanthemum Leaf Dioscorea, Dioscoreaceae, Dioscorea genus, perennial winding herb, also known as Mexican Dioscorea. Dioscorea japonica is mostly distributed in the central part of the Gulf Coast and the southern part of the Pacific coast. It is suitable for growing in areas with an altitude of 1000-1500 meters, an annual average temperature of 25-27 °C, an annual relative humidity of 86%, and an annual precipitation of 1300-1500 mm. Chrysanthemum Dioscorea is known for its high content of diosgenin in its rhizomes. In the 1970s, my country introduced it into Xishuangbanna, Yunnan Province for the first time and the trial planting was successful. Xishuangbanna is located on the edge of the northern tropics, so it has a tropical monsoon rainfore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/15A01G24/10
CPCA01H4/008A01H4/005A01H4/002A01G24/15A01G24/10A01G24/28
Inventor 宋文芹田卫华
Owner 天津博奥聚能生物科技有限公司
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