Extraction method of raw total saponins from fresh dioscorea plants

A technology of total saponins and Dioscorea, applied in the field of extraction of crude total saponins, can solve the problems of sufficient solvent contact, affecting the extraction rate and extraction time of the target extract, and achieve the effect of improving the extraction rate

Inactive Publication Date: 2013-08-07
万绍平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the target extract cannot be released from the cells, so it cannot be fully contacted with the solvent, which greatly affects the extraction rate and extraction time of the target extract.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting crude total saponins from fresh yam plants comprises the following steps.

[0020] (1) Crush 100 g of the rhizome of fresh yams into granules, then store them for natural fermentation, and use them as raw materials. The fermentation time is 12 hours.

[0021] (2) Put the fermented raw material into the container, irradiate it with microwave for 40 seconds, and the temperature of the raw material rises to 60°C.

[0022] (3) Further pulverize the raw materials after microwave irradiation, and pulverize them into particles with a size of 40-100 mesh.

[0023] (4) Soak the particles obtained in step 3 in a 65% ethanol solution for 1 minute, and then filter out the mixed solution.

[0024] (5) After the remaining filter residue was irradiated by microwave for 30 seconds, it was soaked again in 65% ethanol solution for 1 minute, and then the mixed solution was filtered out.

[0025] (6) Repeat the process of step 5 for 3 times.

[0026] (7) After c...

Embodiment 2

[0028] A method for extracting crude total saponins from fresh yam plants comprises the following steps.

[0029] (1) Cut 150g of fresh yam rhizomes into flakes, store them for natural fermentation, and use them as raw materials. The fermentation time is 24 hours.

[0030] (2) Dehydrate the fermented raw materials for a while, put them in a container, and irradiate them with microwaves for 90 seconds, and the temperature of the raw materials rises to 90°C.

[0031] (3) The raw material after microwave irradiation is further pulverized into particles with a size of 60-120 mesh.

[0032] (4) Soak the particles obtained in step 3 in a 95% ethanol solution for 3 minutes, and then filter out the mixed solution.

[0033] (5) After the remaining filter residue was irradiated by microwave for 70 seconds, it was soaked again in 85% ethanol solution for 3 minutes, and then the mixed solution was filtered out.

[0034] (6) Repeat the process of step 5 for 3 times.

[0035] (7) After ...

Embodiment 3

[0037] A method for extracting crude total saponins from fresh yam plants comprises the following steps.

[0038] (1) Cut 300 g of fresh yam rhizomes into flakes or crush them into granules, and then store them for natural fermentation as raw materials. The fermentation time was 36 hours.

[0039] (2) Put the fermented raw material into a container, and irradiate it with microwaves for 150 seconds. When the temperature of the raw material rises to 85°C, stop the microwave irradiation.

[0040] (3) The raw material after microwave irradiation is further pulverized into particles with a size of 140 mesh.

[0041] (4) Soak the particles obtained in step 3 in a 90% methanol solution for 5 minutes, and then filter out the mixed solution.

[0042] (5) After the remaining filter residue was irradiated by microwave for 80 seconds, it was soaked again in 75% methanol solution for 5 minutes, and then the mixed solution was filtered out.

[0043] (6) Repeat the process of step 5 for 4...

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PUM

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Abstract

The invention relates to an extraction method of raw total saponins and in particular to an extraction method of raw total saponins from fresh dioscorpea plants. The extraction method of the raw total saponins from fresh dioscorpea plants mainly comprises the following steps: crushing, fermenting, radiating with microwave, performing secondary crushing, immerging into a solvent, filtering a mixedsolution and recycling the solvent. Compared with the traditional extraction method, the extraction method provided by the invention adopts a method of secondary crushing after microwave radiation, so that cellular content is quickly expanded by virtue of microwave radiation to cause sufficient expansion of cell walls accordingly, and secondary crushing is carried out to wholly disintegrate the cell walls, so that the cellular content is completely released and sufficiently contacted with the solvent, therefore, the extract can be sufficiently dissolved in the solvent, the extraction rate of a target extract is greatly improved, and the content of total saponins in a material can be over 95 percent.

Description

technical field [0001] The invention relates to a method for extracting crude total saponins, in particular to a method for extracting crude total saponins from fresh yam plants. Background technique [0002] Saponin is a relatively complex glycoside compound in the plant kingdom. Saponin is composed of saponin and sugar. It is soluble in water, soluble in ethanol and methanol, and hardly soluble in ether or benzene. Currently commonly used saponin extraction techniques include: cold extraction with water or aqueous solvents, heating extraction, ultrasonic extraction, supercritical extraction, etc. They have a common feature that the extracted plant and its by-products are subdivided and mixed with a solvent in a container for a period of time, and the saponins diffuse into the solvent. The conventional extraction method uses the solvent to enter the cell through the cell wall, and after the extract is dissolved in the solvent, it is brought out from the cell through the ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00A61K36/8945
Inventor 万绍平郑小非
Owner 万绍平
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