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Applications of dioscin and Sophora alopecuroides total alkali in preparation of snail killing agents

A technology of diosgenin and molluscicide, which is applied in the field of molluscicide preparation, can solve problems such as water pollution, achieve the effects of no environmental pollution, simple production and preparation process, and enhanced molluscicide efficacy

Active Publication Date: 2020-01-14
SICHUAN COLLEGE OF ARCHITECTURAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the currently used molluscicides will cause water pollution, the inventors have conducted long-term in-depth research and found that diosgenin has a significant molluscicidal effect when used to control snail hazards in water areas, thus completing the present invention

Method used

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  • Applications of dioscin and Sophora alopecuroides total alkali in preparation of snail killing agents
  • Applications of dioscin and Sophora alopecuroides total alkali in preparation of snail killing agents
  • Applications of dioscin and Sophora alopecuroides total alkali in preparation of snail killing agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Turmeric Diosgenin Extract

[0031] Dry the turmeric and crush it, add 75% ethanol solution to extract at 80°C for 6 hours, and dry the extract by rotary evaporation to form a powder. HPLC detection shows that the content of diosgenin is 72.7%.

[0032] The extraction of diosgenin in turmeric can be done by alcohol extraction or biological enzyme preparation. In this example, a simple and non-toxic ethanol aqueous solution extraction method is used.

Embodiment 2

[0034] Total Alkaline Extraction of Sophora Sophora

[0035] Grind the bitter beans into powder, pass through a 60-mesh sieve, soak in hydrogen chloride with a mass fraction of 0.5 for 12 hours, filter and take the filtrate, concentrate under reduced pressure to form a paste, dissolve it in an ethanol solution with a volume fraction of 85%, and let it stand for 12 hours to take the filtrate , concentrated under reduced pressure into a paste, alkalized with ammonia water to pH 9-10, added NaCl to saturation, extracted several times with chloroform, collected chloroform, placed in rotary evaporation at 50°C and dried into powder, TLC detection containing Sophora flavescens Alkaline, oxymatrine, sophocarpine, oxidized sophocarpine, sophoramine, sophodine, HPLC detection, the total alkaloid content of Sophora japonica in the powder is 43.75%.

Embodiment 3

[0037] Dioscin saponin laboratory dipping experiment to kill molluscs

[0038] Experimental Materials:

[0039] Oncomelania: Collected from Dantu Oncomelania breeding waters in Zhenjiang, Jiangsu, snails with 7-8 spirals, no schistosomiasis infection, negative and healthy snails.

[0040] Experimental method: Dip to kill molluscs Experiment:

[0041] Laboratory: the diosgenin powder obtained in Example 1 is respectively configured into 10, 15, 20, 25, 30mg / L mass concentrations of medicinal liquids with dechlorinated tap water, and each mass concentration medicinal liquid is divided into 3 groups on average, poured respectively Put into 500ml beaker, every cup 300ml, put into respectively 30 of the selected Oncomelania snails in every beaker, be provided with dechlorinated tap water blank control group. Room temperature is 23±1°C, observe the climbing rate for 3 hours, and after soaking for 24 hours, 48 ​​hours, and 72 hours respectively, take a cup of snails from each group...

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Abstract

The invention discloses applications of dioscin in treatment of water area snail harm, provides a successful path for development of a novel efficient low-toxicity snail killing product, and further relates to a snail killing preparation prepared by compounding Sophora alopecuroides total alkali and dioscin. According to the invention, the product is compounded by using the plant preparations, isenvironmentally-friendly, is safe to people and livestock, does not generate resistance, can reach the indexes required by WHO and health schistosomiasis prevention management department, is a novel efficient low-toxicity snail killing product, can replace the existing snail killing chemical niclosamide, and is worthy of application and popularization.

Description

technical field [0001] The invention relates to the technical field of preparation of molluscicides, in particular to the application of diosgenin and total alkaloids of Sophora sophora in the preparation of molluscicides. Background technique [0002] Drug molluscicide is a method of killing snails by using chemical substances that are toxic to snails in an appropriate way. It is time-saving, labor-saving, quick-acting, and reusable. It is currently the main method of molluscicide in my country. The development and production of domestic molluscicides in my country began in the 1950s and 1960s. Up to now, a variety of effective molluscicides have been screened out from more than 2,000 compounds and pesticides, such as insecticide, bromoacetamide, nicotinamide, and insecticide. , metaldehyde, boron ore powder, etc., but due to various reasons such as targeting, environmental pollution, or poor killing effect, they have not been fully popularized and applied. Niclosamide is t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N65/20A01P9/00
CPCA01N43/90A01N65/00A01N65/20Y02A50/30
Inventor 丁陆军刘裕红
Owner SICHUAN COLLEGE OF ARCHITECTURAL TECH
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