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Method for preparing diosgenin by liquid state fermentation of dioscorea zingiberensis

A technology of diosgenin and diosgenin scutellaria, which is applied to the field of liquid fermentation of diosgenin scutellaria scutellariae to prepare diosgenin, and can solve problems such as water pollution, environmental pollution, and increased chemical oxygen demand

Active Publication Date: 2021-02-05
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem of the acid hydrolysis method is: a large amount of starch and cellulose have not been comprehensively utilized, and its hydrolysis leads to a significant increase in the chemical oxygen demand (COD) of the filtrate, and the obtained hydrolyzate is directly washed with water repeatedly to neutrality, resulting in serious Environmental Pollution and Water Pollution
However, these methods are still unable to get rid of acid hydrolysis, and the environmental pollution caused by a large amount of waste water, waste acid and by-products is inevitable, and the application prospect is limited.

Method used

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  • Method for preparing diosgenin by liquid state fermentation of dioscorea zingiberensis
  • Method for preparing diosgenin by liquid state fermentation of dioscorea zingiberensis
  • Method for preparing diosgenin by liquid state fermentation of dioscorea zingiberensis

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, preparation of diosgenin by liquid fermentation of Fusarium sp.

[0035] 1. The preparation of the culture medium used in this embodiment

[0036] Potato dextrose agar medium (PDA medium): Peel potatoes, cut 200g into small pieces, add water and boil for 30min, filter through 4 layers of gauze, add 20g of glucose, 15g of agar, distilled water to 1000mL, boil and mix, 121℃ Sterilize for 20 minutes to obtain PDA medium.

[0037] Seed liquid medium: Seed liquid medium (PDB medium): Peel potatoes, cut 200g into small pieces, add water and boil for 30min, filter with 4 layers of gauze, add 20g of glucose, distill water to 1000mL, boil and mix, 121℃ Sterilize for 20 minutes under the same conditions to obtain PDB medium.

[0038] Liquid fermentation medium (medium containing Dioscorea scutellaria): 21g wheat bran, 2.1g Na 2 HPO 4 , 8.4gKH 2 PO 4 , 26.5g rhizome of Dioscorea scutellaria, 1.9g Tween-80, natural pH, distilled water to 1000mL. Sterilize at 12...

Embodiment 2

[0059] Example 2, Optimization of Microbial Transformation of Yellow Ginger to Prepare Diosgenin Liquid Fermentation Medium

[0060] The liquid fermentation medium used in Example 1 above was obtained through the following optimization experiments.

[0061] In this study, Fusarium sp. CPCC 400226 was used as the experimental strain, and the yield of diosgenin was used as the index. Through the biotransformation mediated by active microorganisms, the response surface optimization method was used to conduct the Plackett analysis on seven factors. -Burman design screened 3 significant factors, and conducted Box-Behnken design experiments on them, and used Design-Expert 8 analysis software to conduct regression analysis to obtain the optimal combination.

[0062] details as follows:

[0063] 1 Experimental materials and methods The experimental materials, culture methods and analysis methods involved are the same as in Example 1.

[0064] 2 Experimental design method

[0065] 2...

Embodiment 3

[0074] Example 3, optimization of liquid state fermentation conditions for preparation of diosgenin by microbial transformation of turmeric

[0075] The liquid fermentation conditions used in the above-mentioned Example 1 were obtained through the following optimization experiments.

[0076] In this study, Fusarium sp. CPCC 400226 was used as the experimental strain, and the yield of diosgenin was used as the index. Through the biotransformation mediated by active microorganisms, the Plackett- Three significant factors were screened by Burman design, and a central composite design experiment was performed on them, and the optimal combination was obtained by regression analysis using Design-Expert 8 (trial version) analysis software.

[0077] details as follows:

[0078] 1 Experimental materials and methods The involved experimental materials, culture methods and analysis methods are the same as in Example 1.

[0079] 2 Experimental design method

[0080] 2.1 The Plackett-Bu...

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Abstract

The invention discloses a method for preparing diosgenin by liquid state fermentation of dioscorea zingiberensis. The method for preparing the diosgenin comprises the following steps of culturing fusarium fungus by using a liquid culture medium containing dioscorea zingiberensis to obtain the diosgenin, wherein the fusarium fungus is a fusarium strain (Fusarium sp.) CPCC 400226. Experiments provethat the yield of the diosgenin is 2.48 + / -0.21% after the fusarium sp. CPCC 400226 is cultured for 11 days at 28 DEG C by adopting the liquid fermentation culture medium disclosed by the invention ina liquid fermentation manner. The method can be used for clean production of diosgenin.

Description

technical field [0001] The invention relates to a method for preparing diosgenin by liquid-state fermentation of diosminthus scutellariae. Background technique [0002] Diosgenin (diosgenin, known as diosgenin in the industry) is an important intermediate in the synthesis of steroid hormone drugs, known as "medicinal gold", and has a huge market demand. At the same time, diosgenin has physiological functions such as anti-cancer, anti-inflammation, anti-virus, anti-skin aging, anti-coagulation and anti-thrombosis. my country is a major exporter of diosgenin, accounting for more than 50% of the world's total output. Dioscorea zingiberensis C.H.Wright, also known as turmeric, is a unique medicinal plant in my country, which is the most important medicinal source plant for producing diosgenin, and diosgenin is also the most critical active substance in Dioscorea zingiberensis. The 3-position glycosylated steroidal saponin exists, and the rhizome part with the highest content ca...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12N1/14C12R1/77
CPCC12P33/20C12N1/14
Inventor 余利岩刘万仓向海波张涛庞旭刘红宇苏静
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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