Application of dioscin in prevention and treatment on pine nematode diseases

A technology of diosgenin and nematodes, applied in the application field of diosgenin in the prevention and treatment of pine nematode diseases, can solve the problems of fast transmission, difficult control, strong pathogenicity, etc., and achieve the effect of easy degradation

Active Publication Date: 2020-06-19
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is characterized by strong pathogenicity and fast death of hosts; rapid spread, and often caught off guard; once it occurs, it is difficult to control. For a long time, the control of pine wood nematode disease has revolved around removing dead wood, killing long beetles with chemicals, and luring However, the current chemical agents are all highly toxic and high-residue pesticides, which are very easy to damage trees and ecology. The environment is damaged, so seeking natural and effective biological control nematicides is one of the research hotspots in the prevention and control of pine nematode diseases

Method used

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  • Application of dioscin in prevention and treatment on pine nematode diseases
  • Application of dioscin in prevention and treatment on pine nematode diseases
  • Application of dioscin in prevention and treatment on pine nematode diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Determination of the inhibitory activity of different diosgenins and matrine on the hatching of pine nematode eggs

[0027] Pick mature pine nematodes with consistent color and size under the dissecting microscope, disinfect them with 2% sodium hypochlorite for 3-6 minutes, rinse them with sterile water for 3 times, put them into a 20-well plate, and add 10 mature pine nematodes to each treatment , add 1ml of test diosgenin solution (I, II, III) and 1ml of test matrine solution respectively, the test solution is prepared with sterilized water, and each treatment is repeated 4 times, compared with sterile water, set 26 Cultivate at ℃, observe and record the number of hatched nematodes on 4d, 8d, and 12d respectively, and calculate the inhibition rate of hatching of nematode eggs.

[0028] Table 1: Egg hatching inhibition rate after the same concentration of dioscin and matrine are processed S: dioscin K: matrine

[0029]

[0030] The results showed that di...

Embodiment 2

[0031] Embodiment 2: Determination of the poisonous activity of diosgenin to the second instar larvae of pine nematode

[0032] Using liquid soaking method: Add 1mL of the test solution to the 20-well plate, the test solution is prepared with sterilized water, then add 30-50 nematodes for the test, place in a 26°C incubator, and wait for 1d and 2d , 3d Check and record the number of live nematodes and dead nematodes under an inverted microscope, and calculate the respective corrected mortality rates. In addition, the treated 3d were resuscitated with clear water for 2d, checked again under an inverted microscope and recorded the number of live nematodes and dead nematodes, and calculated the corrected mortality rate for 2d of resuscitation. Each treatment was repeated 4 times, and sterile water was used as control.

[0033] Table 2: Corrected mortality after the same concentration of dioscin and matrine S: dioscin K: matrine

[0034]

[0035] The results showed that the c...

Embodiment 3

[0036] Embodiment 3: forest farm carries out diosgenin control pine nematode disease test

[0037] The age of the pine tree is 20 years. The diameter at breast height is 14-20cm, and the tree height is 5-7m. The tree species are mainly black pine and masson pine.

[0038] Adopt the method of tree trunk instillation, in late March, select a drill bit with a diameter of 5mm, and use an electric drill to drill a hole at a downward angle of 45° on the trunk at a distance of 40 to 50 cm from the ground. The depth of the hole is about 5-6cm, and there is one infusion hole for each tree, and the total amount of instillation agent is 0.5ml per centimeter base diameter of the trunk. Inject 5% dioscin I, 5% dioscin II, 5% dioscin III and 5% matrine into each hole with a medical infusion set, seal the hole with mud after injection, and repeat three times for each dose.

[0039] Afterwards, the growth of the plants was regularly observed, and it was found that the pine trees treated with...

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Abstract

The invention belongs to the field of biological prevention and treatment, and particularly relates to an application of dioscin to prevention and treatment on pine nematode diseases. The dioscin disclosed by the invention is a steroid compound formed by connecting spirostane type diosgenin and a glycogen connected to a 3-site hydroxyl group through beta-1, 3 glucosidic bonds, and the dioscin comprises dioscin polyphyllin and gracillin. The dioscin can be used as an active pharmaceutical ingredient added into a pesticide carrier or composition to be prepared into a pesticide, and then the dioscin is used for preventing and treating the pine nematode disease by adopting a common pesticide formulation and a using method. The dioscin disclosed by the invention is derived from tubers of dioscorea zingiberensis serving as a dioscorea plant and is harmless to the human body and easy to degrade in environment. Compared with a conventional chemical nematocide matrine, the dioscin has the characteristics of no toxicity and no residue, has better prevention and treatment effects on pine nematode diseases, and meets the requirements of green, environment-friendly and sustainable agriculturalproduction and development.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to the application of diosgenin in preventing and controlling pine nematode diseases. Background technique [0002] Pine wilt disease, also known as pine wilt disease, is a devastating epidemic of pine trees, caused by pine wood nematodes, and brings huge ecological disasters to forestry production. Under normal circumstances, pine xylophilus parasitizes inside pine wood. The nematode has a body length of 1 mm, and the overall appearance is slender. Every year when summer comes, the young shoots of newly grown pine trees will be gnawed by longhorns, and pine xylophilus can quickly enter the forest tree through the gnawed tree skin, and then stay inside the pine tree. Once the pine wood is infected, the epithelial cells and resin ducts of the forest wood will be quickly destroyed, resulting in the loss of transpiration of the pine wood, so that the whole plant shows a s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P5/00
CPCA01N43/90
Inventor 田杰华蔡海金志敏吕华伟
Owner ZHEJIANG UNIV OF TECH
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