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Penicillium fungus M1 and application thereof to increase of saponins yield in fermentation process of ginseng or American ginseng

A fermentation process, technology of the genus Penicillium, applied in the direction of fungi, fermentation, microbial-based methods, etc., can solve the problems of high price, shortage of cultivation land, etc., and achieve the effect of increasing yield

Active Publication Date: 2013-02-27
都晓伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, ginsenosides are mainly isolated from plants such as ginseng and American ginseng. Due to the physiological characteristics of ginseng and American ginseng, it usually takes four to six years to be harvested for medicinal purposes, whether wild or cultivated, and is vulnerable to climate, cultivation conditions, and pests and diseases. Influenced by the impact, there is gradually a shortage of cultivated land and an increasingly expensive situation

Method used

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  • Penicillium fungus M1 and application thereof to increase of saponins yield in fermentation process of ginseng or American ginseng
  • Penicillium fungus M1 and application thereof to increase of saponins yield in fermentation process of ginseng or American ginseng
  • Penicillium fungus M1 and application thereof to increase of saponins yield in fermentation process of ginseng or American ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The separation and purification of embodiment 1 fungus M1

[0041] 1 Experimental instruments and materials

[0042] 1.1 Materials

[0043] The ginseng medicinal material was purchased from Harbin Pharmaceutical Group Shiyitang Pharmaceutical Co., Ltd., and was identified as the dried root and rhizome of Araliaceae plant ginseng (Panax ginseng C.A.Mey) by Professor Du Xiaowei from the Pharmacy Teaching and Research Office of Heilongjiang University of Traditional Chinese Medicine.

[0044] 1.2 Instruments

[0045] AB204-N electronic analytical balance (Mettler-Toledo Shanghai Co., Ltd.); Portable pressure steam sterilizer (Wuxi Changjiang Medical Equipment Co., Ltd.); Class 100 clean bench (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory ); SPX-150C constant temperature and humidity box (Shanghai Boxun Industrial Co., Ltd. Equipment Factory).

[0046] 1.3 Utensils and reagents

[0047] Utensils: scissors, tweezers, gauze, filter paper, absorbent cotton, ...

Embodiment 2

[0064] The strain identification of embodiment 2 active fungi

[0065] 1. Morphological identification of active fungi

[0066] 1 Experimental materials

[0067] 1.1 Experimental material: fungus M1 isolated from ginseng.

[0068] 1.2 Instrument reagents: Motic BA-400 digital microscope image system, Motic Company; lactic acid, chemically pure, Tianjin Kaitong Chemical Reagent Co., Ltd.; phenol, chemically pure, Jilin Jihua Company; glycerin, chemically pure, Jilin Jihua Company Company; water-soluble aniline blue, Shanghai Specimen Model Factory.

[0069] 2 Experimental methods

[0070] 2.1 Morphological identification of strains

[0071] 2.1.1 Preparation of specimen slices

[0072] 2.1.1.1 Slide culture

[0073] Take a piece of circular filter paper with a diameter of about 7cm, lay it on the bottom of a petri dish with a diameter of 9cm, put a U-shaped glass rod on it, put a clean glass slide and a cover glass on it, and cover the petri dish Cover for sterilization....

Embodiment 3

[0131] Example 3 Research on the Changes of Total Saponin and Monomer Saponin Production of Ginseng Medicinal Materials During M1 Fungal Fermentation

[0132] 1. Study on the change of total saponin production of ginseng medicinal materials in the process of fungal fermentation

[0133] 1 Experimental materials

[0134] 1.1 Instrument

[0135] Model 722 UV-Vis Spectrophotometer (Shanghai Spectrum Instrument Co., Ltd.)

[0136] KQ-500DE Medical Numerical Control Ultrasonic Extractor (Kunshan Ultrasonic Instrument Co., Ltd.)

[0137] RE-52 rotary evaporator (Shanghai Botong Technology Co., Ltd.)

[0138] SHB-Ⅲ circulating water type multi-purpose vacuum pump (Zhengzhou Great Wall Science, Industry and Trade Co., Ltd.)

[0139] AB204-N Electronic Analytical Balance (Mettler-Toledo Shanghai Co., Ltd.)

[0140] SPX-150C constant temperature and humidity box (Shanghai Boxun Industrial Co., Ltd. Equipment Factory)

[0141] 1.2 Drugs and reagents

[0142] Ginsenoside reference ...

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Abstract

The invention discloses a strain of Penicillium fungus M1 and application thereof to increase of saponins yield in a fermentation process of ginseng or American ginseng, and belongs to the technical field of biotechnology. According to the invention, functional fungus is used for fermentation cultivation of ginseng or American ginseng; the content of saponins before and after the fermentation are analyzed and compared; and finally a bacterial strain capable of significantly increasing saponins yield in the fermentation process of the two herbs is obtained. The strain is identified as Penicillium fungus (Penicillium sp.), and named after Penicillium fungus M1, and has a preservation number of CGMCC NO.6359. The strain can be used in fermentation production of ginseng or American ginseng; and the content of saponins in fermentation products fermented by the strain is greatly improved. Therefore, according to the present invention, Penicillium fungus has broad application prospects in increasing saponins yield in the fermentation process of ginseng or American ginseng.

Description

technical field [0001] The invention relates to a Penicillium fungus and its new use in the field of fermentation, in particular to its new use in increasing the yield of saponins in the fermentation process of ginseng or American ginseng. Belongs to the field of biotechnology. Background technique [0002] Ginseng is the dried root and rhizome of Panax ginseng C.A.Mey, a plant of the genus Panax ginseng (Panax ginseng C.A.Mey) in the Araliaceae family. Cell growth and many other functions. As a traditional precious Chinese medicine, the ginsenosides contained in ginseng have many significant pharmacological activities. American ginseng is the dried root of Panax quinquefolium L., a plant of the genus Panax quinquefolium L. in the family Araliaceae. It is native to Canada and the United States in North America. Deficiency, internal heat, coughing, phlegm and blood, deficiency heat and fatigue, thirst, dry mouth and throat embolism, can lower blood sugar, lower blood fat, ...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12N1/14C12R1/80
Inventor 都晓伟陈照宇
Owner 都晓伟
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