Application of biocontrol strain FZ01-GFP capable of expressing GFP to targeted pest epidemiology

Abstract

The invention discloses application of a biocontrol bacterium paecilomyces fumosoroseus strain capable of expressing green fluorescent protein (GFP) to the targeted pest epidemiology. According to the biocontrol bacterium, through protoplast transformation technology, an expression plasmid vector pCT74-GFP with green fluorescent protein marks is converted into a genome of the paecilomyces fumosoroseus strain FZ01, and the biocontrol strain FZ01-GFP capable of expressing the GFP is obtained. The bacterial strain is used for researches on the targeted pest epidemiology, the biocontrol strain FZ01-GFP is released, collection is conducted at a fixed time and location, the number of targeted pests is recorded, sick pest samples are collected, PCR molecular detection identification is conducted on the sick pest samples through a pair of specific primers of the plasmid vector pCT74-GFP, data are analyzed, and the plasmid vector pCT74-GFP and tendency of targeted pest infection of the biocontrol strain FZ01-GFP are determined; the problem that it is difficult to identify the pathogenesis of the targeted pests in the researches on the biocontrol bacterium epidemiology in the prior art is solved; the application has the advantages of being simple in step, convenient to operate and capable of effectively monitoring and objectively reflecting the targeted pest occurrence and development rules of the biocontrol bacterium paecilomyces fumosoroseus strain and the like.

Description

technical field [0001] The invention relates to the field of research on the epidemiology of pathogenic fungi of crop pests, in particular to the application of a biocontrol strain Paecilomyces fumigatus FZ01-GFP capable of expressing GFP in the epidemiology of target pests. Background technique [0002] In recent years, my country has paid great attention to the biological control of agricultural pests. However, the current basic research on bio-control bacteria is relatively weak, especially the research on the epidemiology of target pest pathogenic fungi lacks effective and reasonable technical means, which seriously hinders the utilization and development of bio-control bacteria. [0003] Paecilomyces fumosoroseus (Paecilomyces fumosoroseus) belongs to the class Deuteromycetes, the order Shellomyces, and the family Calicilaceae. It has a wide geographical distribution and diverse insect hosts, and is a pathogenic fungus of important pests such as aphids and whiteflies. ...

AI Technical Summary

Problems solved by technology

However, the research on the infection and epidemiology of the target pests by the biocontrol bacteria is still blank. The most important thing is that it is impossible to easily and effectively identify the etiology of the pests, especially for the epidemic law and control of a specific strain in this genus. Evaluation, there is still a lack of fast and reliable technology for large-scale detection applications

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