Paecilomyces lilacinus granule and preparation method thereof

A technology of Paecilomyces lilacinus and granules, applied in the field of pesticides, can solve the problems of unsatisfactory control effect and low control effect

Inactive Publication Date: 2017-02-08
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, disease-resistant varieties or drugs are often used to prevent and control nematode diseases. When drugs are used for prevention and control, the control effect is often relatively low, and the control effect is not ideal
For example, if 90% crystalline

Method used

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  • Paecilomyces lilacinus granule and preparation method thereof
  • Paecilomyces lilacinus granule and preparation method thereof
  • Paecilomyces lilacinus granule and preparation method thereof

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preparation example Construction

[0027] In the present invention, the preparation method of Paecilomyces lilacinus comprises the following steps:

[0028] 1) activating Paecilomyces lilacinus species;

[0029] 2) inoculating the activated Paecilomyces lilacinus in the Paecilomyces lilacinus liquid culture medium for liquid culture, culturing for 55-75 hours to stop fermentation, and obtaining the first-grade seed liquid of Paecilomyces lilacinus;

[0030] 3) inoculating the primary seed liquid of Paecilomyces lilacinus obtained in the step 2) into the solid medium of Paecilomyces lilacinus, and performing solid culture to obtain Paecilomyces lilacinus.

[0031] The activation of the Paecilomyces lilacinus strains in the present invention is to inoculate the preserved Paecilomyces lilacinus strains in an eggplant-shaped bottle equipped with a nutrient agar medium, and cultivate them at 25-30° C. for 10-14 hours for activation culture.

[0032] After the activation, in the present invention, the activated Paec...

Embodiment 1

[0056] Preparation of Paecilomyces lilacinus: Inoculate the preserved Paecilomyces lilacinus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 12 hours for activation; The inoculation amount of % was inoculated in the liquid medium after 121°C sterilization for 35min, and liquid culture was carried out in a fermenter. The specific culture conditions were: temperature was 28°C; pH value was 6.9; The ventilation rate was 1V / V min; the cultivation was stopped after 65h, and the first-grade seed liquid of Paecilomyces lilacinus was obtained; the obtained first-grade seed liquid of Paecilomyces lilacinus was inoculated in a solid medium with a 20% inoculation amount, Add distilled water to the solid medium to make the water content of the solid medium 50%, and then carry out solid culture. The culture conditions are: the culture temperature is 28°C, the culture humidity is 80% for the first three days, and the culture humidity is...

Embodiment 2

[0058] The preparation of Paecilomyces lilacinus granules: in parts by weight, 10 parts of talcum powder, 65 parts of kaolin, 10 parts of Paecilomyces lilacinus prepared in Example 1, 10 parts of glucose and 5 parts of cornstarch were divided into 4 piles, mix the 1st pile of talcum powder with the 1st pile of kaolin, then mix with the 1st pile of Paecilomyces lilacinus, then mix with the 1st pile of glucose, then mix with the 1st pile of corn starch, and then mix with the 2nd pile of Paecilomyces lilacinus Pile talc powder and mix, and so on, until all the raw materials are mixed, and then continue to mix for 60 minutes to obtain a mixture; the mixture is pulverized by airflow to obtain a 15 μm powder; Under the condition of ℃, it is manufactured into 1200 μm granules to obtain Paecilomyces lilacinus granules.

[0059] The quality control index of the Paecilomyces lilacinus granule prepared by the embodiment 2 of table 1

[0060]

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Abstract

The invention provides a Paecilomyces lilacinus granule. The Paecilomyces lilacinus granule comprises 5-15 parts of a medicinal active component which is Paecilomyces lilacinus, 10-25 parts of an assistant, 50-90 parts of a medicine carrier and 15-25 parts of water. The effective spore amount of the Paecilomyces lilacinus granule is 1.8-2.2 * 10<8> cfu/g, the competitor rate is not greater than 0.1%, and the shedding rate is not greater than 3%. The Paecilomyces lilacinus granule has the advantages of high efficiency, low toxicity, stable insecticidal effect, safety to people, animals and crops, and high safety. An experiment result shows that the control effect of Paecilomyces lilacinus reaches up to 78% or above.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to a Paecilomyces lilacinus granule and a preparation method thereof. Background technique [0002] Nematodes can invade and parasitize plants. The normal growth and development of infected and parasitic plants are affected by the absorption of nutrients in the body by nematodes, and the secretions of nematode metabolism can stimulate the cells and tissues of host plants, resulting in plant deformities. Most plant nematodes attack the underground parts of plants, such as roots, tubers, etc., and the roots can be manifested as nodules, necrosis, stubby roots, and clusters, resulting in a large reduction in yield or quality of crops. Common plant nematode diseases include root-knot nematode disease, soybean cyst nematode disease, wheat grain nematode disease, sweet potato stem nematode disease, rice stem-point nematode disease, citrus semi-puncture nematode disease, etc. [0003] ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/12A01P5/00
CPCA01N25/12A01N63/30A01N2300/00
Inventor 吴俊杰李肖宇曾升华石仕福梁小文石峥罗建辉李英武罗双燕王根豪严艺波
Owner JIANGXI TIANREN ECOLOGY
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