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Paecilonyces variotii bacterial strain having high virulence to citrus psylla and application thereof

A technology of Paecilomyces wannerii and citrus psyllids, applied in the field of biological control, to achieve the effects of high spore germination rate, easy preparation, and large spore production

Active Publication Date: 2016-01-20
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Beauveria bassiana (Balsamo) Vuillemin, Isariafumosorosea (Wize) BrownetSmith, Hirsutellacitriformis Speare, Lecanicillium lecanii (Zimmermann) Viégas and Metarhizium anisopliae ( Metchnikoff) Sorokin reported on the prevention and treatment of citrus psyllids, but so far in China, only Zhang Yanxuan et al. have carried out experimental research on the use of Amblyseius cucurbita carrying Beauveria bassiana to control citrus psyllids

Method used

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  • Paecilonyces variotii bacterial strain having high virulence to citrus psylla and application thereof
  • Paecilonyces variotii bacterial strain having high virulence to citrus psylla and application thereof
  • Paecilonyces variotii bacterial strain having high virulence to citrus psylla and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] Isolation and screening of embodiment 1 citrus psyllid pathogenic bacteria

[0027] (1) Isolation and purification of pathogenic bacteria

[0028] The diseased citrus psyllids were collected from Gourita japonica in Tianhe District, Guangzhou City, Guangdong Province, and operated in a laboratory ultra-clean workbench. The citrus psyllid was first soaked in 70% ethanol for 30s, then soaked in 0.1% mercury liter for 1min, and finally rinsed with sterile water for 3 times. Use sterile filter paper to dry the water on the worm body, and then put it on the potato dextrose agar (PDA) medium (the preparation method is: weigh 200g potatoes, wash, peel and chop them, add 1000mL of water and boil for 20-30min, two Filter with a layer of gauze, add 20g of glucose and 15g of agar, fully dissolve and replenish water to 1000mL, then pack into Erlenmeyer flasks, sterilize under high pressure at 121°C for 20min).

[0029] Place the petri dish in a microbial incubator for constant te...

Embodiment 2

[0034] Identification of embodiment 2 bacterial strain GZMS-11

[0035] 1. Morphological characteristics of strain GZMS-11: strain GZMS-11 grows well on potato dextrose agar (PDA) medium, cultured at a constant temperature at 27°C, observes the growth of colonies every day and records the color and shape of colonies. Inoculate the strain GZMS-11 into the center of another PDA medium plate, insert the sterilized coverslip (1cm×1cm) obliquely into the medium about 1cm away from the inoculation point, and culture at a constant temperature of 27°C for 5 days After the hyphae climbed onto the cover glass, the cover glass was taken out and placed under an optical microscope to observe the hyphae and spore morphology of strain GZMS-11.

[0036] figure 1 It is the colony characteristic of strain GZMS-11 cultured on PDA medium for 10 days. It can be seen that the colonies of the strain GZMS-11 are round and fluffy, and the colonies are white at first, and later turn yellowish brown w...

Embodiment 3

[0042] The pathogenicity determination of embodiment 3 bacterial strain GZMS-11 to citrus psyllid

[0043] (1) The lethal medium concentration and lethal medium time of strain GZMS-11 to citrus psyllids

[0044] After the strain GZMS-11 was inoculated on the PDA solid medium for 10 days, the cultured colonies were rinsed with sterile water, and the conidia suspension was collected by filtration with sterile gauze, and serially diluted with sterile water to obtain concentrations of 1.0×10 8 spores / mL, 1.0×10 7 spores / mL, 1.0×10 6 spores / mL, 1.0×10 5 spores / mL, 1.0×10 4 spores / mL conidia suspension, and an appropriate amount of Tween-80 was added thereto to a final concentration of 0.1% (v / v). Put the healthy citrus psyllid in the conidia suspension containing 0.1% (v / v) Tween-80 of the above-mentioned concentrations as described in Example 1 for infiltration treatment, and then put it in a light incubator at 27±0.5°C (L:D=14:10, RH>90%) culture, and the citrus psyllid trea...

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Abstract

The invention discloses a Paecilonyces variotii bacterial strain having high virulence to citrus psylla and an application thereof and relates to the technical field of biological prevention and control. The bacterial strain is Paecilonyces variotii GZMS-11, preserved in China Center for Type Culture Collection on October 29, 2015 with the preservation number CCTCC NO: 2015651. The Paecilonyces variotii GZMS-11 has high virulence to citrus psylla, can be developed into biopesticide for biological prevention and control of citrus psylla. Compared with existing chemicals for prevention and control of citrus psylla, the Paecilonyces variotii GZMS-11 has the advantages of being highly efficient, environment-friendly, high in persistence and not easy to cause drug resistance, and has no pollution to the environment and results in no residue. The bacterial strain of the Paecilonyces variotii GZMS-11 grows fast, has high sporulation yield and high germination rate of spores, is easy to prepare, and has good market perspective.

Description

technical field [0001] The invention belongs to the technical field of biological control, and particularly relates to a Paecilomyces variotii strain with strong pathogenicity to citrus psyllids and an application thereof. Background technique [0002] Citrus Huanglongbing is the most devastating disease in the citrus industry. It can infect a variety of citrus plants. There is no effective therapeutic agent and no disease-resistant (tolerant) varieties available. Citrus Huanglongbing is currently spreading across the world. The gradual spread of growing areas is a major threat to the world citrus industry. Citrus psyllid (Diaphorina citrikuwayamae) belongs to the family Psyllidae of the order Homoptera. It mainly harms Rutaceae plants such as murella and citrus. Adults and nymphs gather in new shoots to suck juice. Shedding seriously affects plant growth and is one of the important pests on citrus. The citrus psyllid is the only insect vector of HLB, and the frequent occu...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/79
Inventor 宋晓兵彭埃天程保平凌金锋周娟陈霞
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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