D-type toxigenic pasturella multocida vaccine strain for porcine atrophic rhinitis and application of strain

A technology for Pasteurella multocida and strains, which is applied in the field of porcine atrophic rhinitis D type toxin-producing Pasteurella multocida vaccine strains, which can solve the problem of damage to the normal structure and function of the respiratory tract, susceptibility to other pathogens, production of Speed ​​down and other problems, to ensure the specificity and immune efficacy, the production of vaccines is simple, and the effect of reducing production costs

Active Publication Date: 2017-11-10
天康生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] PAR is a recessive disease. It generally does not show obvious systemic symptoms or cause death clinically, but it can cause carcass grade reduction or pollution, production speed reduction, and stiff pigs. It can damage the normal structure and function of the respiratory tract and make the body resist. It is very easy to infect other pathogens, such as mycoplasma, haemophilus, swine influenza virus, porcine reproductive respiratory syndrome virus, etc., which can cause pig respiratory diseases and increase the mortality of pigs
At present, the main prevention and treatment measures for porcine atrophic rhinitis are vaccines and drugs. With the increase of bacterial drug resistance, effective drugs are lacking

Method used

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  • D-type toxigenic pasturella multocida vaccine strain for porcine atrophic rhinitis and application of strain
  • D-type toxigenic pasturella multocida vaccine strain for porcine atrophic rhinitis and application of strain
  • D-type toxigenic pasturella multocida vaccine strain for porcine atrophic rhinitis and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Isolation and identification of type D toxigenic Pasteurella multocida TK-MD8 strain.

[0056] 1 Isolation and cultivation of strains: inoculate the brain-heart infusion agar medium with the nasal swabs and lung tissues collected from pigs with symptoms of porcine atrophic rhinitis, incubate at 37°C for 18-24 hours and then observe, pick out 1-2 mm in diameter, Clear, smooth colonies were subjected to Gram staining. Gram-stained coccus parvum was a suspicious colony, which was subcultured and purified for further biochemical and PCR identification.

[0057] 2 strain identification

[0058] 2.1 Biochemical identification: the isolated suspicious strain was inoculated on fresh blood brain heart infusion agar medium and grew well, showing small colonies like water droplets without hemolysis. Inoculate suspicious strains into biochemical culture tubes and culture at 36-38°C for 24-48 hours. The strains ferment ornithine, mannitol, glucose and sucrose, but do not ferment m...

Embodiment 2

[0064] Preparation of Pasteurella multocida inactivated vaccine (TK-MD8 strain).

[0065] 1 Preparation of bacterial solution for seedling production

[0066] Aseptically unpack Pasteurella multocida TK-MD8 strain, dissolve it in liquid medium, inoculate it in fresh blood solid medium, and culture it at 36-38°C for 18-24 hours, pick out small colonies in the form of water droplets, For the colony without hemolysis, inoculate the liquid culture medium at 36-38°C, culture for 18-24 hours, conduct a pure inspection according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and use it as the seed solution if it passes the inspection. Take the seed liquid to inoculate the liquid culture medium, put it in a constant temperature shaking incubator at 36-38°C for 16-24 hours, and conduct pure inspection and live bacteria count according to the appendix of the current "Chinese Veterinary Pharmacopoeia".

[0067] 2 Inactivation of bacteria solution

[0068] Formaldehy...

Embodiment 3

[0074] Inspection of Pasteurella multocida Inactivated Vaccine (TK-MD8 Strain)

[0075] 1 Vaccine testing

[0076] 1.1 Sterility test

[0077] Tested according to the appendix of the current "Chinese Veterinary Pharmacopoeia", it should grow aseptically.

[0078] 1.2 Safety inspection

[0079] Five healthy susceptible pigs aged 3 to 4 weeks were used to inject 4ml / head of pig vaccine into the muscle behind the ears and neck. Continuous observation for 14 days, daily temperature measurement, all test pigs should not appear any adverse reactions caused by vaccination.

[0080] 1.3 Effectiveness test

[0081] Five healthy susceptible pigs aged 3 to 4 weeks were used to inject 2ml of the vaccine into the neck behind each ear, and the second immunization was carried out in the same way and with the same dose 21 days later. Twenty-one days after the second immunization, 5 piglets were selected from each of the immunization group and the control group, respectively, and the Past...

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Abstract

The invention relates to the technical field of prevention and treatment of animal diseases, in particular to a D-type toxigenic pasturella multocida vaccine strain for porcine atrophic rhinitis and an application of the strain. The strain is pasturella multocida TK-MD8 and collected in CGMCC (China General Microbiological Culture Collection Center) in the collection No. CGMCC No.10394 on Jan.27th, 2015. A univalent vaccine prepared from the D-type toxigenic pasturella multocida vaccine strain is simple to produce, safe and efficient.

Description

technical field [0001] The invention relates to the technical field of animal disease prevention and control, in particular to a porcine atrophic rhinitis type D toxigenic Pasteurella multocida vaccine strain and application thereof. Background technique [0002] Pasteurella multocida (Pm) type D is one of the main pathogens of swine atrophic rhinitis. The swine atrophic rhinitis caused by this bacteria is called Progressive Atrophic Rhinitis (PAR). The main features of the disease are chronic rhinitis, facial deformation and turbinate atrophy. After the suckling piglets are infected with this disease, in addition to causing the deformation, atrophy or disappearance of the turbinates and even the nasal cavity, it can also cause calcium metabolism disorders in the whole body, resulting in growth retardation and reduced feed utilization in piglets, sometimes accompanied by acute and chronic bronchitis, leading to death of piglets. [0003] PAR is a recessive disease. It gener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31A61K39/102A61P31/04A61P11/02C12R1/01
CPCA61K39/02A61K39/102A61K2039/521A61K2039/70C07K14/285C12N1/205C12R2001/01
Inventor 郝成武何传雨何海韩瑞贺笋李延涛
Owner 天康生物制药有限公司
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