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Streptococcus suis and application thereof

A technology of Streptococcus suis and microbial strains, applied in the direction of bacteria, antibacterial drugs, veterinary vaccines, etc., can solve serious and unfavorable clinical disease prevention and treatment, pathogenic bacteria resistance and other problems, to achieve strong pathogenicity and good protection effect, effect of good immunogenicity

Inactive Publication Date: 2017-07-07
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many drugs developed earlier can have a good therapeutic effect on suis streptococcosis. However, with the development of breeding scale, the use of a large number of antibacterial drugs and the unreasonable use of drugs during the drug withdrawal period have caused the drug resistance of pathogenic bacteria. More and more serious, more and more unfavorable to the prevention and treatment of clinical diseases

Method used

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  • Streptococcus suis and application thereof
  • Streptococcus suis and application thereof
  • Streptococcus suis and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the isolation and identification of Streptococcus suis

[0022] 1.1 Disease data collection

[0023] The disease material came from a 2-month-old nursery pig that died of severe pneumonia, dyspnea and neurological symptoms in a large pig farm in Jun County, Henan Province in November 2016.

[0024] 1.2 Preparation of medium

[0025] TSB liquid medium: Dissolve 30 g of TSB broth powder (Tryptic Soy broth, tryptone soybean broth) in 1000 mL of ultrapure water, sterilize at 115 °C for 15 min, add 50 mL of fetal bovine serum, sterile 1% NAD (Nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide, coenzyme Ⅰ) 1mL.

[0026] TSA solid medium: Dissolve 40g of TSA agar powder (Tryptic Soy Agar, tryptone soybean agar) in 1000mL of ultrapure water, sterilize at 115°C for 15min, add 50mL of fetal bovine serum, 1mL of sterile 1% NAD; Store at ℃ for later use.

[0027] 1.3 Isolation and cultivation of strains

[0028] Aseptically collect the brain tis...

Embodiment 2

[0064] Embodiment 2, the preparation of vaccine, safety and efficacy test

[0065] 2.1 Preparation of vaccine

[0066] Inoculate Streptococcus suis strain HNSS1 into TSB liquid medium, place it in a shaker at 37°C for culture, collect the culture after 18 hours, measure the concentration, and adjust the number of bacteria in the culture to 2×10 9 CFU / mL, add formaldehyde to a final concentration of 0.2%, inactivate at 37°C for 12h. Add aluminum hydroxide adjuvant to the inactivated culture at a volume ratio of 1:1, and mix to prepare the inactivated Streptococcus suis vaccine.

[0067] 2.2 Vaccine Safety Test

[0068] Ten 14-day-old healthy nursery pigs were selected and divided into 2 groups with 5 pigs in each group. Vaccine group: intramuscular injection of 4 mL of this vaccine in the neck; control group: intramuscular injection of 4 mL of sterilized normal saline in the neck. The body temperature of the animals was measured, and the clinical manifestations were observe...

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Abstract

The invention discloses a Streptococcus suis and application thereof. The Streptococcus suis is Streptococcus suis HNSS1 and is preserved in China Generall Microbiological Culture Collection Center with the preservation number of CGMCC NO:13334, the preservation data of November 22th, 2016 and the preservation address of No.3, Courtyard 1, Beichen West Road, Chaoyang District of Beijing. The strain HNSS1 is separated from a brain tissue of a nursery pig dead due to occurrence of typical respiratory symptoms and neurologic symptoms, no other bacteria grow during separation on a TSA solid culture medium, only streptococcus suis grows, and proliferation titer in a TSB liquid culture medium is high and reaches up to more than 10<9>CFU / mL. The strain HNSS1 disclosed by the invention has stronger pathogenicity on the nursery pig, results in pathogenesis and death of the nursery pig and has good immunogenicity.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a strain of Streptococcus suis and its application. Background technique [0002] Streptococcus suis (Streptococcus Suis, SS) is a second-class animal disease stipulated by the state. Diseases such as meningitis and sepsis. According to the capsular polysaccharide antigen of Streptococcus suis, it can be divided into 35 serotypes, namely types 1-34 and type 1 / 2. Type 1, type 2, type 1 / 2, type 7 and type 9 are more pathogenic to pigs. Among them, Streptococcus suis type 2 is the most prevalent and most pathogenic serotype, followed by type 9, type 7, and type 1. In recent years, the suis streptococcosis caused by type 2 streptococcus is on the rise. The disease is mainly acute sepsis, with sudden onset and quick death, which has caused huge economic losses to the pig industry, and Streptococcus suis type 2 can cause human infection and death. In addition, the diseas...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/09A61P31/04C12R1/46
CPCA61K39/092A61K2039/521A61K2039/552A61K2039/55505C12N1/205C12R2001/46
Inventor 徐引弟张青娴王治方郎利敏李海利朱文豪焦文强张立宪游一王克领
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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