Streptococcus suis and application thereof

A technology of Streptococcus suis and microbial strains, applied in the direction of bacteria, antibacterial drugs, veterinary vaccines, etc., can solve serious and unfavorable clinical disease prevention and treatment, pathogenic bacteria resistance and other problems, to achieve strong pathogenicity and good protection effect, effect of good immunogenicity

Inactive Publication Date: 2017-07-07
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many drugs developed earlier can have a good therapeutic effect on suis streptococcosis. However, with the development of breeding scale, the use of a large number of antibacterial drugs and the unreasonabl

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Streptococcus suis and application thereof
  • Streptococcus suis and application thereof
  • Streptococcus suis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1. Isolation and identification of Streptococcus suis

[0022] 1.1 Disease collection

[0023] The disease feed came from a 2-month-old nursery pig who died of severe pneumonia, dyspnea and neurological symptoms in a large pig farm in Jun County, Henan Province in November 2016.

[0024] 1.2 Preparation of medium

[0025] TSB liquid medium: Dissolve 30g of TSB broth powder (Tryptic Soy broth) in 1000mL ultrapure water, sterilize at 115℃ for 15min, add 50mL fetal bovine serum, sterile 1% NAD (Nicotinamide) adeninedinucleotide, nicotinamide adenine dinucleotide, coenzyme Ⅰ) 1mL.

[0026] TSA solid medium: Dissolve 40 g of TSA agar powder (Tryptic Soy Agar, Tryptic Soy Agar) in 1000 mL of ultrapure water, sterilize at 115°C for 15 minutes, add 50 mL of fetal bovine serum, and 1 mL of sterile 1% NAD; Store at ℃ for later use.

[0027] 1.3 Isolation and cultivation of strains

[0028] The brain tissues of the sick pigs were collected aseptically and inoculated on TSA solid med...

Example Embodiment

[0064] Example 2. Preparation, safety and efficacy test of vaccine

[0065] 2.1 Preparation of vaccine

[0066] The Streptococcus suis strain HNSS1 was inoculated into TSB liquid medium and placed in a shaker at 37°C for culture. The culture was collected after 18 hours, the concentration was measured, and the number of bacteria in the culture was adjusted to 2×10 9 CFU / mL, add formaldehyde to a final concentration of 0.2%, and inactivate at 37°C for 12h. The inactivated culture was added with aluminum hydroxide adjuvant in a volume ratio of 1:1 and mixed to prepare an inactivated vaccine of Streptococcus suis.

[0067] 2.2 Safety test of vaccine

[0068] Select 10 14-day-old healthy nursing pigs and divide them into 2 groups with 5 pigs in each group. Vaccine group: neck intramuscular injection of 4mL of this vaccine; control group: neck intramuscular injection of 4mL sterile saline. The animal body temperature was measured and the clinical manifestations were observed for a total ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a Streptococcus suis and application thereof. The Streptococcus suis is Streptococcus suis HNSS1 and is preserved in China Generall Microbiological Culture Collection Center with the preservation number of CGMCC NO:13334, the preservation data of November 22th, 2016 and the preservation address of No.3, Courtyard 1, Beichen West Road, Chaoyang District of Beijing. The strain HNSS1 is separated from a brain tissue of a nursery pig dead due to occurrence of typical respiratory symptoms and neurologic symptoms, no other bacteria grow during separation on a TSA solid culture medium, only streptococcus suis grows, and proliferation titer in a TSB liquid culture medium is high and reaches up to more than 10<9>CFU/mL. The strain HNSS1 disclosed by the invention has stronger pathogenicity on the nursery pig, results in pathogenesis and death of the nursery pig and has good immunogenicity.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a strain of Streptococcus suis and its application. Background technique [0002] Streptococcus suis (Streptococcus Suis, SS) is a second-class animal disease stipulated by the state. Diseases such as meningitis and sepsis. According to the capsular polysaccharide antigen of Streptococcus suis, it can be divided into 35 serotypes, namely types 1-34 and type 1 / 2. Type 1, type 2, type 1 / 2, type 7 and type 9 are more pathogenic to pigs. Among them, Streptococcus suis type 2 is the most prevalent and most pathogenic serotype, followed by type 9, type 7, and type 1. In recent years, the suis streptococcosis caused by type 2 streptococcus is on the rise. The disease is mainly acute sepsis, with sudden onset and quick death, which has caused huge economic losses to the pig industry, and Streptococcus suis type 2 can cause human infection and death. In addition, the diseas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A61K39/09A61P31/04C12R1/46
CPCA61K39/092A61K2039/521A61K2039/552A61K2039/55505C12N1/205C12R2001/46
Inventor 徐引弟张青娴王治方郎利敏李海利朱文豪焦文强张立宪游一王克领
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap