The invention discloses a pharmaceutical composition for preventing and treating a poultry air sac inflammation and a preparation method thereof .The pharmaceutical composition for preventing and treating the poultry air sac inflammation is prepared from nervate twayblade herb, fig fruit, cortex fraxini, platycodon roots, licorice roots, betel nuts, tsaoko amomum fruit, officinal magnolia bark, Chinese thorowax roots, baical skullcap roots, raw lotus leaves, herba houttuyniae, pine needles, opuntia dillenii Haw., radix sophorae flavescentis, fructus forsythiae, mulberry leaves, honeysuckle flowers, common fenugreek seeds, dried tangerine peel, Chinese wax gourd peel, peach flowers, Chinese lobelia herb, radix gentianae and radix ophiopogonis by weight .The traditional Chinese medicine preparation has the complementary and synergistic effects on treating pathology of the poultry air sac inflammation, is mild in drug property, free of irritation to respiratory mucosae, capable of rapidly relieving respiratory symptoms caused by air sac inflammation type respiratory diseases and repairing the respiratory mucosae, stable in drug efficacy and lasting in effect and can obviously cure or relieve poultry air sac inflammation symptoms, decrease poultry death rate and reduce losses.