Metarhizium anisopliae plant for controlling curculio chinensis chevrolat larvae and application of metarhizium anisopliae plant

A technology of Metarhizium anisopliae and Camellia oleifera, applied in the fields of application, pesticides, fungi, etc., to achieve good production properties, strong secondary infection ability, and good application prospects

Active Publication Date: 2015-04-29
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the search, there is no report on the use of entomogenic fungi to control Camellia oleifera larvae. For this reason, Metarhizium anisopliae with strong pathogenicity to its larvae was screened out ( Metarhizium anisopliae ) strains, it is of great practical significance to provide excellent strains and bacterial agents for production

Method used

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  • Metarhizium anisopliae plant for controlling curculio chinensis chevrolat larvae and application of metarhizium anisopliae plant
  • Metarhizium anisopliae plant for controlling curculio chinensis chevrolat larvae and application of metarhizium anisopliae plant
  • Metarhizium anisopliae plant for controlling curculio chinensis chevrolat larvae and application of metarhizium anisopliae plant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Metarhizium anisopliae ( Metarhizium anisopliae ) Identification of FJMa201101

[0023] Morphological identification

[0024] Cultivate the isolated and purified Metarhizium anisopliae strain on a flat PPDA medium with a diameter of 90mm at 25±1°C. After 4-5 days, pick a white hyphae preparation and observe the hyphae and meristems of the strain under a microscope Spore peduncle morphology; after 10-12 days, pick mature conidia and make slices to observe the morphology and size of the conidia. Observe and record the morphological characteristics of the colony for about 15 days. The colony texture of this strain is cotton-wool, and the colony is white at the beginning, and gradually expands, and dark green conidia are produced on it and distributed in a ring. Mycelia branched, latticed, colorless and smooth, 2.3μm~3.2μm wide, conidiophores solitary or branched, with 2~5 columnar sporulation cells at the top of the branches, on which basal sporozoites are produced ...

Embodiment 2

[0034] Metarhizium anisopliae ( Metarhizium anisopliae ) Biological properties of FJMa201101

[0035] Metarhizium anisopliae ( Metarhizium anisopliae) FJMa201101 was inoculated in the center of the PPDA plate medium, cultured in an incubator at 25±1°C, and the colony diameter was measured once a day by the cross method, with 3 plates (repeated) for each treatment, and observed for 15 days in total. At the same time, record the start time of sporulation. On the 15th day (sporulation is basically over), use a puncher with a diameter of 1.5 cm to punch a small plate from the center of the colony to the edge 1 / 2, take out the plate and place it in a 0.3 ‰ Tween-80 sterile water in the Erlenmeyer flask, shake to fully disperse the spores and properly dilute, measure the concentration of spores with a hemocytometer and convert it into the amount of sporulation per unit area. The determination was repeated three times on each plate, and the average value was taken as the numb...

Embodiment 3

[0040] Metarhizium anisopliae ( Metarhizium anisopliae ) Determination of pathogenicity of FJMa201101 to Camellia oleifera larvae

[0041] Scrape a certain amount of Metarhizium anisopliae from the PPDA plate ( Metarhizium anisopliae ) FJMa201101 spores were placed in sterile 0.3‰ Tween-80 solution, and prepared into 10 7 Spores / mL of spore suspension (the suspension is Metarhizium anisopliae FJMa201101 inoculum); soak healthy Camellia oleifera larvae in the spore suspension for 10 seconds, and then put them back into the sterilized soil in the insect culture box In this method, 30 camellia oleifera larvae were inoculated and repeated 3 times; treated with 0.3‰ Tween-80 sterile aqueous solution as a control. After treatment, place the insect rearing box in a dark 25±1°C constant temperature incubator, observe and record the death of camellia oleifera larvae every day, observe the growth of mycelia and sporulation of the dead insects in moisturizing culture (the growth ...

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Abstract

The invention discloses a metarhizium anisopliae plant for controlling curculio chinensis chevrolat larvae and application of the metarhizium anisopliae plant. The metarhizium anisopliae plant provided by the invention is named as FJMa 201101, and is collected in China General Microbiological Culture Collection Center on 27th, October, 2014, with a collection number of CGMCC No.9780. The metarhizium anisopliae plant is high in growth speed and strong in sporulation capacity, and has the high pathogenicity on curculio chinensis chevrolat larvae, and when the curculio chinensis chevrolat larvae are inoculated by using a spore suspension of 10<7>spores / ml, the LT50 (lethal time of 50%) of the curculio chinensis chevrolat larvae is 1.65 days. A fungal preparation produced by using the plant has a good control effect on the curculio chinensis chevrolat larvae, is capable of effectively controlling the population density of the curculio chinensis chevrolat larvae, is a biocontrol strain with a potential value on the aspect of controlling the curculio chinensis chevrolat, and has the positive meaning in promotion of high quality and high yield of oil-tea camellia.

Description

technical field [0001] The invention belongs to the field of biological control, and relates to a strain of Metarhizium anisopliae and its application, in particular to a strain of Metarhizium anisopliae with high pathogenicity to larvae of Camellia oleifera and its application. Background technique [0002] Camellia oleifera (Oil-tea Camellia) is a unique woody edible oil tree species in my country, mainly distributed in the Yangtze River Basin and 14 provinces (cities, districts) south of it. Camellia elephant Curculio chinensis Chevrolat, also known as Tea Seed Weevil and Camellia oleifera, belongs to the Weevil family Weevil. It is the most serious pest that harms Camellia oleifera fruit, and it is also a national forestry dangerous pest (State Forestry Administration, 2013). The larvae eat nuts in the fruit, which seriously reduces the yield of tea seeds and affects the quality of tea oil. According to the survey, the fruit drop rate after Camellia oleifera damage can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 何学友蔡守平杨希曾丽琼黄金水
Owner FUJIAN ACAD OF FORESTRY
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