34results about How to "Strong sporulation ability" patented technology

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

The invention relates to a Metarhiziumanisopliaevar. anisopliae strain CQMa421 of which the CGMCC (China General Microbiological Culture Collection Center) NO is 4609 and an application thereof in the control of rice leaf roller. The production traits of the strain are good and can grow fast and have high spore-producing capability; and the sporulation rate is up to 6.8%, and the strain has high toxicity. The pesticidal fungal agent produced by using the strain can be used to effectively control the number of the rice leaf rollers.

The invention discloses a beauveria bassiana DSXJ-07, which has been preserved with the accession number being CGMCC No.7192. The sequence of an internal trasicribed spacer of the beauveria bassiana DSXJ-07 is represented as the Seq ID No.7192. The invention also provides the application of the strain in prevention and treatment on pests on graminaceous crops, wherein the application is used for preparing a drug for preventing and treating the pests on graminaceous crops. The graminaceous crops include paddy rice, corn, sugarcanes, wheat, barley or forage grass. The pests comprise lissorhoptrus oryzophilus kuschel and asiatic corn borer. A pest killing fungus preparation prepared from the strain can effectively control the population quantity of the lissorhoptrus oryzophilus kuschel and asiatic corn borer.

The invention discloses a beauveria bassiana strain having pathogenicity on cyclophragma undans and an application thereof. The beauveria bassiana strain is named as beauveria bassiana FJBb201202 and preserved in a common microorganism center of China committee for culture collection of microorganisms on March 19, 2015, and the preservation number is CGMCC No. 10641. The strain is high in growth rate, high in spore producing capacity and good in production property; meanwhile, the pathogenicity on the cyclophragma undans is high, the larva death rate reaches 100 percent 15 days after spore suspension with the concentration 107 spores/mL is inoculated into third instar larvae of the cyclophragma undans, and LT50 (the median lethal time) is 5.83. A fungal preparation produced by the strain has the good control effect on the cyclophragma undans and can effectively decrease the forest pest density of the cyclophragma undans; the strain is a biocontrol strain which has the application value on controlling of the cyclophragma undans and has the positive significance for reducing the harm of the cyclophragma undans.

The invention discloses a strain of Aspergillus niger Ty-3 and application thereof. The strain has been deposited in the China Center for Type Culture Collection on July 8, 2011 with the accession number of CCTCC NO.: M 2011241. The mycelia of the strain can adhere to and wind around the mycelia of tobacco black shank and parasitize on the mycelia of tobacco black shank and grow; through long time parasitic growth, the mycelia of the strain can kill and decompose pathogens, thereby exerting a strong inhibitory effect on the growth of pathogens. The Aspergillus niger strain is applicable to preparation of bactericides used for controlling tobacco diseases.

The invention provides a metarhizium flavoviride bacterial strain (Metarhizium flavoviride.minus) CQMf1072 with the preservation number of CGMCCNO.4717 and the ITS1-5.8S-ITS2 sequence of SEQ ID NO.1. The metarhizium flavoviride bacterial strain CQMf1072 which is directly separated and screened from naturally infected rice planthopper has strong sporulation capability, and the sporulation rate can be up to 2.5%; the metarhizium flavoviride bacterial strain has good production trait and is rapid to grow; meanwhile, the metarhizium flavoviride bacterial strain has high insecticidal activity on the rice planthopper and has a good insecticidal effect; the LT50 (lethal time) and LT90 (lethal time) of the larva of the rice planthopper are respectively 5.8 days and 8.7 days. By using an insecticidal fungal preparation which is manufactured by utilizing the bacterial strain, the population quantity of the rice planthopper is effectively controlled.

The invention relates to a fungi medium which comprises the following raw materials: 15-18 g/L potato extract powder, 3-5 g/L yeast extract powder, 20-22 g/L glucose and 15-20 g/L agar powder. The fungi medium prepared by taking the potato extract powder with stable quality and the yeast extract powder which enriches a fungi growth environment and is convenient for fungi metabolism observation, as raw materials is stable in quality, simple in material preparation, convenient in material getting, easy to prepare and good in operability; when the fungi medium is used for fungi culture, fungi cells with good growth synchronicity, thick and uniform fungi layers and high spore productivity can be obtained and the culture cycle of fungi can be shortened to be only 3-5 days.

The invention relates to (Aspergillus fumigates)Ty-1 screened out from in-situ soil and application of the (Aspergillus fumigates)Ty-1. The depositary institution of the (Aspergillus fumigates)Ty-1 is China Center for Type Culture Collection (CCTCC), the depositary date of the (Aspergillus fumigates)Ty-1 is October 16, 2011, and the depositary number of the (Aspergillus fumigates)Ty-1 is CCTCC M 2011353. According to the invention, the hyphae of (Aspergillus fumigates)Ty-1 can be attached and wound on the hyphae of tobacco black shanks, and the hyphae of pathogenic bacteria are finally decomposed because the protoplasm of the hyphae of the pathogenic bacteria is concentrated and vacuolated and collapses. The cell wall of the attached and wound host hyphae body is adhered by a sucker of the host hyphae body and grows through sucking nutritive substances in the host hyphae body by using the sucker, so that the strong inhibition on the growth of the pathogenic bacteria is generated.

The invention discloses a Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of the Metarhizium anisopliae strain, and belongs to the technical field of biological control. The strain is named Metarhizium anisopliae FJMa201516, and the preservation number is CGMCC No.18123. The strain is high in growth speed and high in spore production capacity, and meanwhile has high pathogenicity to the larvae of the Milionia basalis; after the larvae of the Milionia basalis are inoculated with a 107 spore/mL spore suspension for 15 days, the cumulative deathrate of the larvae reaches 96.7%, and LT50 is 5.01 days; a fungal preparation produced by using the strain has a good control effect on the Milionia basalis, and the population density of the larvaeof the Milionia basalis can be effectively reduced. The strain has potential value in control of the Milionia basalis, and has positive significance in reducing harm caused by the Milionia basalis andreducing the use amount of chemical pesticides.

The invention belongs to the technical field of biological prevention and control of agriculture and forestry pests, and particularly relates to beauveria bassiana, use thereof in preventing and treating tea tree leaf-eating beetles and a fungus agent. The beauveria bassiana is named as FJBb1338 and has the preservation number of CGMCC No.20253. The invention provides the use of the beauveria bassiana in preventing and treating the tea tree leaf-eating beetles. The invention further provides thefungus agent for preventing and treating the tea tree leaf-eating beetles. Beneficial effects are as follows: the beauveria bassiana FJBb1338 has a high growth speed and a strong sporulation capacity and the fungus agent of the beauveria bassiana can be remarkably reduce the number of the leaf-eating beetles in a tea garden. Meanwhile, the strain can be stored in the tea garden for a long time, has high secondary infection capacity and is suitable for biological prevention and treatment of the leaf-eating beetles in the tea garden.

Provided is cladosporium cladosporioides CQBBW3. The preservation number is CGMCC No.11439. The homology between the rDNA-ITS sequence of the cladosporium cladosporioides and the corresponding sequence of the cladosporium cladosporioides reaches up to 95%. The cladosporium cladosporioides CQBBW3 which is directly separated and screened from naturally-infected citrus red mites is high in conidium-producing ability, the conidium-producing quantity reaches 4.3*10<8> conidia/mL on the fourteenth day, the production character is good, growth is fast, and the colony diameter is 6.6125 cm on the fourteenth day. Meanwhile, the citrus red mite killing activity is high, the citrus red mite killing effect is good, LC50 of female adult mites of citrus red mites is 4.66*10<8> conidia/mL, and LT50 of the female adult mites of the citrus red mites is 6.2353 days. An insecticidal fungus preparation produced through the cladosporium cladosporioides effectively controls the colony quantity of the citrus red mites.

The invention provides a novel Aspergillus niger strain QY-002 capable of simultaneously secreting amylase and glucoamylase. The novel Aspergillus niger strain QY-002 has an accession number of CGMCC No. 10650 and is preserved on April 24, 2015. In the conventional fermentation industry, amylase and glucoamylase are separately produced by using different processes. In the conventional sugar industry, liquefaction and saccharification of starch are carried out in two steps under different temperature and pH value conditions and aided by amylase and glucoamylase respectively. Enzyme liquid obtained after fermentation of the novel Aspergillus niger strain has the activity of both amylase and glucoamylase and produces synergism at a same reaction temperature and a same pH value condition, so high-efficiency catalysis is realized in complete hydrolysis of a substrate, i.e., starch, into glucose. Thus, a process of preparing sugar from starch is greatly simplified; reaction time is shortened; product quality is improved; energy consumption is reduced; production cost is lowered; and the novel Aspergillus niger strain has potential and wide application value and is of epoch-making significance to development of the industry of preparation of sugar from starch in China.