34results about How to "Strong sporulation ability" patented technology

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

The invention discloses a Beauveria bassiana DSXJ-07, which has been preserved, and the storage number is CGMCC No.7192, and the sequence of the internal transcriptional spacer is SEQ ID No.7192. The present invention also provides the application of the bacterial strain for preventing and controlling pests on gramineous crops, and as the application of preparing a medicine for preventing and controlling pests on gramineous crops, wherein the gramineous crops are rice, corn, sugarcane, wheat, barley or forage grass. The pests mentioned are rice water weevil and Asian corn borer. The insecticidal fungal preparation produced by the strain can effectively control the populations of the rice water weevil and the Asian corn borer.

The invention relates to a Nomuraea rileyi strain CQNr126, with a preserving number of CGMCC No.4610. The strain provided by the present invention has a strong sporulation capability. On a 3 / 4 SMAY medium plate, the amount of spores reaches 2.2*10<8> spore / cm<3>. The strains have a good productive performance, grow fast, and have a strong insecticidal activity and a good insecticidal effect against rice leaf rollers and chilo suppressalis. Under the effects of the strains, the median lethal time (LT50) of rice leaf rollers and chilo suppressalis are respectively 6.03 days and 6.42 days. With a fungal preparation prepared from the strains, rice leaf roller population and chilo suppressalis population can be effectively controlled.

The invention relates to (Aspergillus fumigates)Ty-1 screened out from in-situ soil and application of the (Aspergillus fumigates)Ty-1. The depositary institution of the (Aspergillus fumigates)Ty-1 is China Center for Type Culture Collection (CCTCC), the depositary date of the (Aspergillus fumigates)Ty-1 is October 16, 2011, and the depositary number of the (Aspergillus fumigates)Ty-1 is CCTCC M 2011353. According to the invention, the hyphae of (Aspergillus fumigates)Ty-1 can be attached and wound on the hyphae of tobacco black shanks, and the hyphae of pathogenic bacteria are finally decomposed because the protoplasm of the hyphae of the pathogenic bacteria is concentrated and vacuolated and collapses. The cell wall of the attached and wound host hyphae body is adhered by a sucker of the host hyphae body and grows through sucking nutritive substances in the host hyphae body by using the sucker, so that the strong inhibition on the growth of the pathogenic bacteria is generated.

The invention discloses wuyiencin spore powder and a production technology thereof. The production technology comprises liquid culture, solid culture, drying, crushing and separation so as to obtain the wuyiencin spore powder, wherein spore number of the wuyiencin spore powder is greater than or equal to 50.0*10<8>, the water content is less than or equal to 8%, the pH value is 4.0-7.0, and the resolution ratio of the effective components is less than or equal to 5%. In the invention, through the expansion culture of liquid phase before solid phase, the spore powder content of the obtained wuyiencin spore powder is greater than 50*10<8> / g, thus the stress resistance of the spores is strong, thereby being favorable for storage and transportation; the wuyiencin spore powder can be used in various modes by directly spraying powder or spraying after being prepared into a liquid preparation, thereby being convenient to use; and the wuyiencin spore powder is particularly suitable for preventing diseases of woods and fruit trees, and has an obvious prevention effect.

The invention discloses a strain of Metarhizium anisopliae for controlling camellia oleifera larvae and its application. The Metarhizium anisopliae strain provided by the present invention is named as Metarhizium anisopliae (Metarhizium anisopliae) FJMa201101, which has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on October 27, 2014, and the preservation number is CGMCC No.9780 . The strain has fast growth rate, strong sporulation ability, and high pathogenicity to the larvae of Camellia oleifera. The LT50 (medium lethal time) to larvae of Camellia oleifera after inoculation with 107 spores / mL larvae was 1.65 days. The fungal preparation produced by the strain has a good control effect on Camellia oleifera and can effectively control the population density of Camellia oleifera.

The invention discloses a spore-forming medium for inducing and destroying anthracnose bacteria. The 1L medium includes naked oats 60-80g, glucose 20-30g, beef extract 10-15g, agar 15-20g, red heart lotus succulent leaves 80-100g and 1L of water; the preparation process includes: weighing, filtering, mixing, and sterilization; the induction process includes: strain activation, mycelium culture, conidia culture and conidia attachment spore induction.

The invention belongs to the technical field of microorganisms, and in particular relates to a high-yielding compound cellulose-degrading enzyme biological control strain, namely Trichoderma asperellum T-1, which has been preserved in China Microbial Bacteria on September 24, 2014 Species Deposit Management Committee General Microbiology Center, the deposit number is CGMCC 9722. The invention also provides a garbage composting inoculant prepared from the T-1 strain of Trichoderma aculeatus and its application in the degradation treatment of organic garbage. The Trichoderma aculeatus involved in the present invention has fast growth, strong sporulation ability and strong pH tolerance, can use various cellulose materials as substrates, is a member of biocontrol bacteria, and can prevent diseases when applied to crops. . The Trichoderma aculeatus T-1 bacterial agent provided by the present invention can be added to organic garbage to reduce the permeate produced by garbage composting, improve the degradation efficiency of cellulose substances in garbage, and improve the quality of garbage composting products.

The invention discloses a spore-producing medium for corn leaf spot fungus, a preparation method and application thereof. The medium includes potatoes, mannitol, agar powder and water, wherein 200 g / L of potatoes, 5-10 g / L of mannitol, and 14 g / L of agar powder. Cultivated on the surface of the spore-forming medium, and cultivated in the dark at 28°C for 6-7 days, a large number of conidia with uniform shape, good germination effect, and high germination rate can be produced on the surface of the medium, and the sporulation yield is about equal to 1.2 to 3.6 times the sporulation yield of PDA medium, and the germination rate can reach more than 90%. The spore-producing culture medium for the corn leaf spot fungus is suitable for the subculture of the pathogen, and can be used in the screening of the pathogenic agent and the determination of the pathogenicity of the pathogen.

The invention discloses a metarhizium strain pathogenic to Laos false blister beetles and application of the metarhizium strain. The metarhizium strain is named as metarhizium anisopliae [FJMa200902, which is stored in China general microbiological culture collection center (CGMCC)], and the collection number is CGMCC No.10642. The strain grows fast and has strong spore formation capability, good production traits and high pathogenicity to larvae of the Laos false blister beetles; after the strain is inoculated by a spore suspension liquid of 107 spores / mL, LT50 (lethal time of 50%) of the strain to the larvae of the Laos false blister beetles is 3.6 days, and infection rate reaches 100%. A fungus preparation made from the strain has good prevention and treatment effects on the Laos false blister beetles, population density of the larvae of the Laos false blister beetles can be reduced effectively, and the metarhizium strain is a biocontrol strain which has application value for prevention and treatment of the Laos false blister beetles.

The invention provides a metarhizium flavoviride bacterial strain (Metarhizium flavoviride.minus) CQMf1072 with the preservation number of CGMCC NO.4717 and the ITS1-5.8S-ITS2 sequence of SEQ ID NO.1. The metarhizium flavoviride bacterial strain CQMf1072 which is directly separated and screened from naturally infected rice planthopper has strong sporulation capability, and the sporulation rate can be up to 2.5%; the metarhizium flavoviride bacterial strain has good production trait and is rapid to grow; meanwhile, the metarhizium flavoviride bacterial strain has high insecticidal activity on the rice planthopper and has a good insecticidal effect; the LT50 (lethal time) and LT90 (lethal time) of the larva of the rice planthopper are respectively 5.8 days and 8.7 days. By using an insecticidal fungal preparation which is manufactured by utilizing the bacterial strain, the population quantity of the rice planthopper is effectively controlled.