(Aspergillus fumigates)Ty-1 and application of (Aspergillus fumigates)Ty-1

A technology of Aspergillus fumigatus and tobacco, applied in the application, chemical for biological control, biocide and other directions, can solve the problems of difficult colonization, poor growth ability of strains, reduced antagonism, etc., and achieves prevention and control of tobacco black shank. Effect

Inactive Publication Date: 2012-07-04
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Penicillium lilacinus ZY-19-2 has a strong ability to produce chitinase, which can decompose the body wall of fly maggots and resist the infection of tobacco black shank. However, it is difficult to colonize foreign strains when applied to the soil.
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Method used

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  • (Aspergillus fumigates)Ty-1 and application of (Aspergillus fumigates)Ty-1
  • (Aspergillus fumigates)Ty-1 and application of (Aspergillus fumigates)Ty-1
  • (Aspergillus fumigates)Ty-1 and application of (Aspergillus fumigates)Ty-1

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Effect test

Embodiment 1

[0046] Example 1 Isolation and screening of tobacco black shank antagonistic bacteria

[0047] 1) Isolation of pathogenic bacteria

[0048] Tobacco black shank was isolated from infected tobacco plants collected in Luoshan County of Xinyang and Wuyang County of Luohe. When the cotton-like mycelium grows around the cigarette block, a little mycelium is picked and purified, and inserted into the PDA slant medium for subsequent use. PDA medium: 200g potato, 20g sucrose, 15-20g agar, 1000ml distilled water, natural pH, pressure 1.05kg / cm2, sterilized at 121.3°C for 20min.

[0049] The micrographs show that the hyphae of the fungus are colorless and without septa; cyst peduncles protrude from the stomata of the diseased tissue, and 1-3 roots are bundled; the sporangia are terminal or lateral, pear-shaped to oval, with papillae, The sporangia can release 5-30 zoospores; the zoospores are nearly round or reniform, colorless, with two lateral flagella.

[0050] 2) Identification of...

Embodiment 2

[0059] Embodiment 2Ty-1 Morphological Observation

[0060] The initial colony of Ty-1 bacteria is flat and white, with sparse hyphae, and the mature colony is black and colorless on the back. The hyphae are colorless and clearly separated, with branches, 3-4 μm in diameter. When mature, the hyphae grow conidiophores, the main trunk and the top of each side branch are stalked, the stalks are in the shape of spindle rods with two pointed ends, and the conidia are spherical or oval like Figure 8 , Figure 9 shown.

Embodiment 3

[0061] Embodiment 3Ty-1 parasitic phenomenon observation

[0062] In the confrontation culture of Ty-1 bacteria and black shank pathogenic bacteria, it was found that Ty-1 bacteria grew vigorously and could produce a large number of short velvet aerial hyphae and black conidia. Ty-1 bacteria can cross the colony junction and directly occupy the growth space of the black shank colony, and can grow on the bacteria, produce spores, and gradually digest the bacteria. Figure 10 shown. The antagonism of the bacterium is manifested as the formation of a bacteriostatic zone or a bacteriostatic zone, which significantly inhibits the growth of pathogenic mycelia, and has obvious nutrient competition and secretion of anti-biological substances to produce antagonism.

[0063] Observing the hyphae at the junction of the colony with a microscope, it was found that the Mycelia of Ty-1 fungus could attach and wrap around the mycelium of blackleg disease as follows: Figure 11 , Figure 12...

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Abstract

The invention relates to (Aspergillus fumigates)Ty-1 screened out from in-situ soil and application of the (Aspergillus fumigates)Ty-1. The depositary institution of the (Aspergillus fumigates)Ty-1 is China Center for Type Culture Collection (CCTCC), the depositary date of the (Aspergillus fumigates)Ty-1 is October 16, 2011, and the depositary number of the (Aspergillus fumigates)Ty-1 is CCTCC M 2011353. According to the invention, the hyphae of (Aspergillus fumigates)Ty-1 can be attached and wound on the hyphae of tobacco black shanks, and the hyphae of pathogenic bacteria are finally decomposed because the protoplasm of the hyphae of the pathogenic bacteria is concentrated and vacuolated and collapses. The cell wall of the attached and wound host hyphae body is adhered by a sucker of the host hyphae body and grows through sucking nutritive substances in the host hyphae body by using the sucker, so that the strong inhibition on the growth of the pathogenic bacteria is generated.

Description

technical field [0001] The invention relates to a microbial strain, in particular to an Aspergillus fumigatus strain screened from in situ soil and its application in the prevention and treatment of tobacco black shank. Background technique [0002] Tobacco black shank is an important soil-borne disease of tobacco, which usually infects tobacco roots, causes diseases of crop roots and even whole plants, and causes significant economic losses. At present, agricultural measures such as disease-resistant varieties and crop rotation are mainly used in production to prevent and control tobacco soil-borne diseases. The Kunming Institute of Botany, Chinese Academy of Sciences has developed a variety (patent number CN01108714.5) resistant to black shank, but due to the variety of disease-resistant genes required for disease-resistant varieties, and the impact of the environment is relatively large, the control effect is not ideal; crop rotation is also One of the effective methods ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/68
Inventor 郭夏丽李红丽王岩李玉红
Owner ZHENGZHOU UNIV
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