Beauveria bassiana strain having pathogenicity on cyclophragma undans and application thereof

A corrugated caterpillar, Beauveria bassiana technology, applied in the application, pesticides, fungi and other directions, can solve problems such as environmental pollution and pest resistance.

Active Publication Date: 2015-11-11
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and control of mottled caterpillars in production mainly adopts chemical control methods, but long-term use of chemical agents will kill a large number of nat

Method used

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  • Beauveria bassiana strain having pathogenicity on cyclophragma undans and application thereof
  • Beauveria bassiana strain having pathogenicity on cyclophragma undans and application thereof
  • Beauveria bassiana strain having pathogenicity on cyclophragma undans and application thereof

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Experimental program
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Effect test

Embodiment 1

[0031] Beauveria bassiana ( Beauveria bassiana ) Identification of FJBb201202

[0032] Morphological identification

[0033] Cultivate the isolated and purified Beauveria bassiana strain on a 90mm-diameter plate PPDA medium at 25±1°C, pick white mycelium slices after 4-5 days, and observe the mycelia and conidia of the strain under a microscope Stem morphology; 10-12 days later, pick mature conidia and make slices to observe the shape and size of conidia. Observe and record the morphological characteristics of the colony for about 15 days. The colonies of this strain are white to milky white, fluffy or cotton-like at first, and milk powder-like spore layer is formed later, the spore layer is thick and uniform, and the center is raised. Branched hyphae, regular, colorless and smooth, sometimes growing in clusters; sporogenous cells (bottle stems) are densely clustered on short and enlarged stalk cells, bottle-shaped, the upper part of the sporogenous cells is zigzag, and th...

Embodiment 2

[0036] Beauveria bassiana ( Beauveria bassiana ) Biological properties of FJBb201202

[0037] Beauveria bassiana ( Beauveria bassiana ) The strain FJBb201202 was inoculated in the center of the PPDA plate medium, cultured in an incubator at 25±1°C, and the diameter of the colony was measured once a day by the cross method, with 3 replicates for each treatment, and a total of 15 days of observation. At the same time, record the start time of sporulation. On the 15th day (sporulation is basically over), use a puncher with a diameter of 1.5cm to punch a small plate from the center of the colony to the edge 1 / 2, take out the plate and place it in a 0.3 ‰ Tween-80 sterile water in the Erlenmeyer flask, shake to fully disperse the spores and properly dilute, measure the concentration of spores with a hemocytometer and convert it into the amount of sporulation per unit area. Three wells were drilled on each plate to measure the spore content, and the average value was taken as th...

Embodiment 3

[0042] Beauveria bassiana ( Beauveria bassiana ) Determination of the pathogenicity of FJBb201202 to the larvae of the mottled caterpillar

[0043] A certain amount of Beauveria bassiana was scraped from the PPDA plate ( Beauveria bassiana ) FJBb201202 spores were placed in sterile 0.3‰ Tween-80 solution, and prepared into 10 7 Spores / mL of spore suspension (the suspension is Beauveria bassiana FJBb201202 inoculum); immerse healthy 3rd instar larvae of the mottled caterpillars in the spore suspension for 10s, and then put them back into the insect culture box to Feed with fresh pine needles, inoculate 30 mottled caterpillar larvae, repeat 3 times; soak in 0.3‰ Tween-80 sterile aqueous solution as a control. After the treatment, place the insect culture box in a dark 25±1°C constant temperature incubator, observe and record the death of the mottled caterpillar larvae every day, observe the growth of hyphae and sporulation of the dead insects in moisturizing culture (visible...

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Abstract

The invention discloses a beauveria bassiana strain having pathogenicity on cyclophragma undans and an application thereof. The beauveria bassiana strain is named as beauveria bassiana FJBb201202 and preserved in a common microorganism center of China committee for culture collection of microorganisms on March 19, 2015, and the preservation number is CGMCC No. 10641. The strain is high in growth rate, high in spore producing capacity and good in production property; meanwhile, the pathogenicity on the cyclophragma undans is high, the larva death rate reaches 100 percent 15 days after spore suspension with the concentration 107 spores/mL is inoculated into third instar larvae of the cyclophragma undans, and LT50 (the median lethal time) is 5.83. A fungal preparation produced by the strain has the good control effect on the cyclophragma undans and can effectively decrease the forest pest density of the cyclophragma undans; the strain is a biocontrol strain which has the application value on controlling of the cyclophragma undans and has the positive significance for reducing the harm of the cyclophragma undans.

Description

technical field [0001] The invention belongs to the field of pest biological control, and relates to a Beauveria bassiana strain and its application, in particular to a Beauveria bassiana strain which has pathogenicity to the larvae of the mottled caterpillar and the application thereof. Background technique [0002] Corrugated caterpillar Cyclophragmaundans (Walker), belongs to Lepidoptera, Satyridae, Miscellaneous caterpillars, is an important leaf-eating pest of various coniferous and broad-leaved forests and fruits, and has a wide range of hosts. In addition to mainly harming Pinus massoniana, it also harms including Dozens of coniferous and broad-leaved trees, including Chinese fir, camellia oleifera, litsea cubeba, chestnut, bayberry, etc., broke out in some areas, and the leaves (needles) of the host plants could be eaten completely during the outbreak. Like a fire, causing great economic losses. [0003] At present, the prevention and control of the mottled caterpi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 蔡守平何学友詹祖仁吴培衍
Owner FUJIAN ACAD OF FORESTRY
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