Induced destruction of anthracnose spore-forming medium, preparation method and method for inducing spore-forming

A culture medium and anthracnose technology, which is applied in the field of plant pathogenic fungi research, can solve the problems of inability to meet the needs of destroying anthracnose research in a short period of time, and the formation of conidia attached spores is small, so as to shorten the research and test period, The effect of high generation rate and saving valuable time

Active Publication Date: 2022-03-18
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Potato dextrose agar (PDA) medium is often used in the prior art for sporulation culture of destroying anthracnose bacteria, but the amount of conidia discs and conidia attached sporogenesis is small, and the time required to obtain a large number of conidia is 10-15 days , unable to meet the needs of artificial research on destroying anthrax bacteria in a short time

Method used

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  • Induced destruction of anthracnose spore-forming medium, preparation method and method for inducing spore-forming
  • Induced destruction of anthracnose spore-forming medium, preparation method and method for inducing spore-forming
  • Induced destruction of anthracnose spore-forming medium, preparation method and method for inducing spore-forming

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Experimental program
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Effect test

Embodiment 1

[0040] Use the destruction of anthracis spore-producing medium of the present invention to cultivate, and the components of the following mass fractions are included in the 1L medium:

[0041]

[0042] Prepare medium:

[0043] 1) Weighing: Weigh naked oats, glucose, beef extract, agar, succulent leaves and water according to the quality, and set aside;

[0044] 2) Grinding and filtering: take the succulent leaves, grind them into a homogenate with a mortar, filter, and take the filtrate;

[0045] 3) mixing the filtrate obtained in step 2) with naked oats, glucose, beef extract and agar obtained in step 1), adding water to dissolve to obtain a solution;

[0046] 4) Sterilize the solution obtained in step 3) at 115°C for 30 minutes, cool to 45°C, add 100 μL of rifampicin with a concentration of 50 μg / mL per liter of medium, mix well, pour it into a petri dish to solidify, and obtain the product Spore culture medium, spare.

[0047]The process of inducing and destroying ant...

Embodiment 2

[0054] Use the destruction of anthracnose spore-producing medium of the present invention to cultivate, and the components comprising the following mass fractions in the 1L medium are:

[0055]

[0056] Prepare medium:

[0057] 1) Weighing: Weigh naked oats, glucose, beef extract, agar, succulent leaves and water according to the quality, and set aside;

[0058] 2) Grinding and filtering: take the succulent leaves, grind them into a homogenate with a mortar, filter, and take the filtrate;

[0059] 3) mixing the filtrate obtained in step 2) with naked oats, glucose, beef extract and agar obtained in step 1), adding water to dissolve to obtain a solution;

[0060] 4) Sterilize the solution obtained in step 3) at 125°C for 20 minutes, cool to 55°C, add 100 μL of rifampicin with a concentration of 50 μg / mL per liter of medium, mix well, pour it into a petri dish to solidify, and obtain the product Spore culture medium, spare.

[0061] The process of inducing and destroying a...

Embodiment 3

[0068] Use the destruction of anthracis spore-producing medium of the present invention to cultivate, and the components of the following mass fractions are included in the 1L medium:

[0069]

[0070] Prepare medium:

[0071] 1) Weighing: Weigh naked oats, glucose, beef extract, agar, succulent leaves and water according to the quality, and set aside;

[0072] 2) Grinding and filtering: take the succulent leaves, grind them into a homogenate with a mortar, filter, and take the filtrate;

[0073] 3) mixing the filtrate obtained in step 2) with naked oats, glucose, beef extract and agar obtained in step 1), adding water to dissolve to obtain a solution;

[0074] 4) Sterilize the solution obtained in step 3) at 120°C for 21 minutes, cool to 50°C, add 100 μL of rifampicin with a concentration of 50 μg / mL per liter of medium, mix well, pour it into a petri dish to solidify, and obtain the product Spore culture medium, spare.

[0075] The process of inducing and destroying an...

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Abstract

The invention discloses a spore-forming medium for inducing and destroying anthracnose bacteria. The 1L medium includes naked oats 60-80g, glucose 20-30g, beef extract 10-15g, agar 15-20g, red heart lotus succulent leaves 80-100g and 1L of water; the preparation process includes: weighing, filtering, mixing, and sterilization; the induction process includes: strain activation, mycelium culture, conidia culture and conidia attachment spore induction.

Description

technical field [0001] The invention relates to the technical field of plant pathogenic fungi research, and more specifically relates to a culture medium for inducing Bacillus anthracis sporulation and a method for inducing sporulation. Background technique [0002] Colletotrichum destructivum has a wide host range and can infect various host plants such as tobacco, sunflower, Indian cowpea, alfalfa, succulent and anthurium, and is the main leaf disease in the process of domestic succulent planting. The anthracnose bacteria mainly survive the winter in diseased tissue or soil in the form of conidia, mycelium or conidia, and spread to plants by airflow at the beginning of the next year. After overwintering, the mycelia and conidia discs can develop into conidia and become the source of primary infection. In addition, the prevalence of anthrax bacteria is most closely related to two factors, temperature and humidity, and usually occurs most seriously in a high-humidity enviro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 姚锦爱黄鹏余德亿
Owner INST OF PLANT PROTECTION FAAS
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