Induced destruction of anthracnose spore-forming medium, preparation method and method for inducing spore-forming
A culture medium and anthracnose technology, which is applied in the field of plant pathogenic fungi research, can solve the problems of inability to meet the needs of destroying anthracnose research in a short period of time, and the formation of conidia attached spores is small, so as to shorten the research and test period, The effect of high generation rate and saving valuable time
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Embodiment 1
[0040] Use the destruction of anthracis spore-producing medium of the present invention to cultivate, and the components of the following mass fractions are included in the 1L medium:
[0041]
[0042] Prepare medium:
[0043] 1) Weighing: Weigh naked oats, glucose, beef extract, agar, succulent leaves and water according to the quality, and set aside;
[0044] 2) Grinding and filtering: take the succulent leaves, grind them into a homogenate with a mortar, filter, and take the filtrate;
[0045] 3) mixing the filtrate obtained in step 2) with naked oats, glucose, beef extract and agar obtained in step 1), adding water to dissolve to obtain a solution;
[0046] 4) Sterilize the solution obtained in step 3) at 115°C for 30 minutes, cool to 45°C, add 100 μL of rifampicin with a concentration of 50 μg / mL per liter of medium, mix well, pour it into a petri dish to solidify, and obtain the product Spore culture medium, spare.
[0047]The process of inducing and destroying ant...
Embodiment 2
[0054] Use the destruction of anthracnose spore-producing medium of the present invention to cultivate, and the components comprising the following mass fractions in the 1L medium are:
[0055]
[0056] Prepare medium:
[0057] 1) Weighing: Weigh naked oats, glucose, beef extract, agar, succulent leaves and water according to the quality, and set aside;
[0058] 2) Grinding and filtering: take the succulent leaves, grind them into a homogenate with a mortar, filter, and take the filtrate;
[0059] 3) mixing the filtrate obtained in step 2) with naked oats, glucose, beef extract and agar obtained in step 1), adding water to dissolve to obtain a solution;
[0060] 4) Sterilize the solution obtained in step 3) at 125°C for 20 minutes, cool to 55°C, add 100 μL of rifampicin with a concentration of 50 μg / mL per liter of medium, mix well, pour it into a petri dish to solidify, and obtain the product Spore culture medium, spare.
[0061] The process of inducing and destroying a...
Embodiment 3
[0068] Use the destruction of anthracis spore-producing medium of the present invention to cultivate, and the components of the following mass fractions are included in the 1L medium:
[0069]
[0070] Prepare medium:
[0071] 1) Weighing: Weigh naked oats, glucose, beef extract, agar, succulent leaves and water according to the quality, and set aside;
[0072] 2) Grinding and filtering: take the succulent leaves, grind them into a homogenate with a mortar, filter, and take the filtrate;
[0073] 3) mixing the filtrate obtained in step 2) with naked oats, glucose, beef extract and agar obtained in step 1), adding water to dissolve to obtain a solution;
[0074] 4) Sterilize the solution obtained in step 3) at 120°C for 21 minutes, cool to 50°C, add 100 μL of rifampicin with a concentration of 50 μg / mL per liter of medium, mix well, pour it into a petri dish to solidify, and obtain the product Spore culture medium, spare.
[0075] The process of inducing and destroying an...
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