Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of Metarhizium anisopliae strain

A technology for Metarhizium anisopliae, orange-banded blue-foot moth, applied in the application, pesticides, fungi and other directions, to achieve the effect of reducing the amount of use, strong spore-producing ability, and significant control effect

Active Publication Date: 2020-03-20
FUJIAN ACAD OF FORESTRY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

After retrieval, there is no report on the use of Metarhizium anisopliae to control the blue-footed moth

Method used

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  • Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of Metarhizium anisopliae strain
  • Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of Metarhizium anisopliae strain
  • Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of Metarhizium anisopliae strain

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1 scarab metarhizium anisopliae ( Metarhizium anisopliae ) Identification of FJMa201516

[0031] Morphological identification:

[0032] The isolated and purified Metarhizium anisopliae FJMa201516 strain was cultured on a flat PPDA medium with a diameter of 90 mm at 25±1°C, and after 4 to 5 days, the white mycelium was picked up to observe the mycelium and distribution of the strain under a microscope. Morphology of sporozoites; after 8-10 days, mature conidia were picked to make slices, and the shape and size of the conidia were observed under a microscope. Observe and record the morphological characteristics of the colony for about 15 days. The colony of this strain is white at the beginning, and the texture is cotton-wool. After 4 to 5 days, dark green conidia are produced on the colony and distributed in a ring. Mycelia branched, latticed, colorless and smooth, 2.2μm~3.3μm wide, conidiophores solitary or branched, with 2~5 columnar sporulation cells at...

Embodiment 2

[0041] Embodiment 2 scarab metarhizium anisopliae ( Metarhizium anisopliae ) Biological properties of FJMa201516

[0042] Metarhizium anisopliae ( Metarhizium anisopliae ) FJMa201516 was inoculated in the center of PPDA plate medium, cultured in an incubator at 25±1°C, and the colony diameter was measured once a day by the cross method, and 3 plates (repeated) were set up for each treatment, and observed for 15 days in total. At the same time, record the start time of sporulation. On the 15th day (sporulation is basically over), use a puncher with a diameter of 1.5 cm to punch a small plate from the center of the colony to 1 / 2 of the edge, take out the plate and place it in a 30 mL container In a triangular flask with 0.3‰ Tween-80 sterile water, oscillate to fully disperse the spores and dilute them appropriately. Measure the spore concentration with a hemocytometer and convert it into the sporulation per unit area. The determination was repeated three times on each plate...

Embodiment 3

[0047] Metarhizium anisopliae ( Metarhizium anisopliae ) Determination of the pathogenicity of FJMa201516 to the larvae of the blue-footed moth

[0048] Collection and rearing of test insects:

[0049] The test insect was the larvae of the orange-banded blue-footed moth. It was collected on May 12, 2019 at the Podocarpus nursery in Youxi County, which was seriously damaged by the orange-banded blue-footed moth. After the larvae were collected, they were brought back to the laboratory, placed in insect cages, and fed with fresh Podocarpus pine leaves. After 1 day, the selection activities were normal, and the 3rd to 4th instar larvae were placed in insect feeding boxes, 15 insects were placed in each feeding box, for the determination of the pathogenicity of Metarhizium anisopliae.

[0050] Pathogenicity determination and analysis

[0051] Scrape a certain amount of Metarhizium anisopliae from the PPDA plate ( Metarhizium anisopliae ) FJMa201516 spores were placed in ster...

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Abstract

The invention discloses a Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of the Metarhizium anisopliae strain, and belongs to the technical field of biological control. The strain is named Metarhizium anisopliae FJMa201516, and the preservation number is CGMCC No.18123. The strain is high in growth speed and high in spore production capacity, and meanwhile has high pathogenicity to the larvae of the Milionia basalis; after the larvae of the Milionia basalis are inoculated with a 107 spore/mL spore suspension for 15 days, the cumulative deathrate of the larvae reaches 96.7%, and LT50 is 5.01 days; a fungal preparation produced by using the strain has a good control effect on the Milionia basalis, and the population density of the larvaeof the Milionia basalis can be effectively reduced. The strain has potential value in control of the Milionia basalis, and has positive significance in reducing harm caused by the Milionia basalis andreducing the use amount of chemical pesticides.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a strain of Metarhizium anisopliae with high pathogenicity to larvae of the blue-footed moth and its application. Background technique [0002] Podocarpus ( Podocarpus macrophyllus ) and cypress ( Podocarpus nagi ) is an important tree species for landscaping and garden landscape in our country, especially podocarpus has great economic value, and a large number of species are introduced from abroad every year in our country. Orange banded blue inch moth ( Milionia basalis Walker) belongs to Lepidoptera (Lepidoptera) Geometridae (Geometridae) blue-footed moth ( Milionia Walker 1854), is an important leaf-eating pest that has occurred on podocarpus and bamboo cypress in southern my country in recent years. When it occurs seriously, it will eat up the leaves of the host plant, leaving only bare branches. In Guangxi, my country , Guangdong, Hainan, Taiwa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/145C12R2001/645
Inventor 何学友蔡守平郑宏曾丽琼宋海天
Owner FUJIAN ACAD OF FORESTRY
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