Curvularia dilatata strain and application thereof

A technology of spores and strains, which is applied to the strains of Curculiocarpus intumescenta and its application field, can solve the problems that there is no report on the research on insecticidal activity and insecticidal mechanism, and achieves strong insecticidal activity, reduced dosage and good insecticidal effect. Effect

Active Publication Date: 2019-01-15
CHONGQING TAIJI MEDICAL RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, domestic and foreign research on T.inflatum mainly focuses on the antibacterial activity of secondary metabolites and the pr

Method used

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  • Curvularia dilatata strain and application thereof
  • Curvularia dilatata strain and application thereof
  • Curvularia dilatata strain and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0043] The isolation of embodiment 1 bacterial strain

[0044]Disinfect the surface of the collected naturally infected bat moth larvae with 70% alcohol, put them in a 25°C incubator and culture them for 3-5 days, so that fresh spores grow on the surface of the insects; under sterile conditions, pick them with an inoculation loop Take a small amount of fresh spores and streak them on the PDA medium plate containing 100 μg / ml ampicillin; turn the streaked culture dish over and place it in a 25°C incubator for cultivation; after 3-4 days, select the typical ones without bacteria Use a transfer needle to pick up a small piece of medium with hyphae at the edge of the colony, transfer it to the center of the culture medium on the slant of the test tube, and culture at a constant temperature of 25°C for 10 days.

Embodiment 2

[0045] The identification of embodiment 2 bacterial strains

[0046] (1) Morphological identification

[0047] The isolated (SCKDC10) strain was inoculated on a PDA plate and cultured at 25°C to observe the morphological characteristics of the colony, the sporulation structure, and the shape and size of the spores. see figure 2 , Inoculated on a PDA plate with the strain SCKDC10 of Curvularia magnum of the present invention, displayed on a PDA medium plate, cultivated at a constant temperature of 25°C, the strain was cultivated for 5 days, and the colony was round and white, fluffy, with a diameter of 2-4cm; the middle of about 7 days of culture Fluffy fluffy, radial fluffy edges, 5-8cm in diameter; 14 days later, the colony diameter is 10-12cm, the colony is still white, the edges are neat, the hyphae are evenly raised, the fluff becomes rare, the back is light yellow, and the back of the colony changes from the initial The yellow and white color gradually turns dark yello...

Embodiment 3

[0053] Example 3 Determination of Indoor Insecticidal Activity of SCKDC10 Strain to Lepidoptera Model Insect Bombyx mori 3rd Instar Larvae and Pupa

[0054] Take the SCKDC10 strain grown on the PDA medium for 7 days, scrape the conidia under aseptic conditions, suspend them in 0.02% Tween 80 sterile water, filter and collect the conidia, and configure 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 5 different concentrations of conidia / mL.

[0055] Take 60 silkworms from the 4th instar silkworm, set up 3 replicates for each strain, 20 heads for each replicate, soak in the conidia suspension prepared in advance for 25 seconds, take it out, absorb the water on the body surface with filter paper and put it into a 150mm petri dish , feed fresh mulberry leaves coated with spore suspension on the back of the leaves, place them in a constant temperature incubator at 28°C for moisturizing culture, and treat silkworm and mulberry leaves with sterile water containing 0.02% Tween-80 as a ...

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Abstract

The invention belongs to the field of microorganisms, in particular to a Curvularia dilatata strain and an application thereof. The strain SCKDC10 (CCTCC M2018701) of Tolypocladium inflatum of the present invention has homology between a rDNA-ITS and Tolypocladium inflatum isolate as high as 99%. The strain SCKDC10 of Curvularia dilatata isolated from the larvae of the bat moth has good productioncharacteristics and rapid growth, and the diameter of the colony after 15 days of cultivation can reach 10-12cm; and has strong insecticidal activity against many kinds of pests at the same time. Thecumulative mortality to larvae of Bombyx mori and Panonychus citri is 78.33% and 59.5% respectively at the concentration of 2.0 * 108 spores/mL on the eighth day. The insecticidal fungicides producedby this strain could effectively control the number of many kinds of insect pests.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of C. inflata and its application. Background technique [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in agriculture as an increase in production and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (i.e. resistance to pesticides, residence of pesticide residues, No matter how rampant Resurgence is, it has also brought a series of ecological and environmental problems. The long-term and extensive use of chemical pesticides has led to an increase in pesticide-resistant pests. In particular, in the past 10 years, cotton bollworms, aphids, diamondback moths, Spodoptera litura, and spider mites have become more resistant to chemical pesticides such as pyrethroid...

Claims

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Application Information

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IPC IPC(8): C12N15/31C12N1/14A01N63/04A01P7/02A01P7/04C12R1/645
CPCA01N63/30C07K14/37C12N1/14C12N1/145C12R2001/645
Inventor 秦少容胡军华卿玉玲陈仕江王帆陈若霓
Owner CHONGQING TAIJI MEDICAL RES INST CO LTD
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