127 results about "Metarhizium sp." patented technology

The invention relates to a metarhiziumanisopliae compound insecticide composition and use thereof. The metarhiziumanisopliae compound insecticide composition is characterized in that: the active ingredients of the composition include metarhiziumanisopliae and a new neonicotinoid insecticide; the density of the metarhiziumanisopliae in the composition is 1 to 10 billion spores per gram; and the new neonicotinoid insecticide content of the composition is 20 mass percent; and the rest content of the composite are pesticide auxiliary ingredients. The composition has an obvious synergistic effect on grubs and is suitable for preventing and controlling plant pests, particularly grubs. The composition can be used on various crops such as lawns, peanut, sweet potatoes and vegetables or in various places and has the advantages of stable insecticide effect, high quick-acting performance, long efficacy duration, safety and environmental protection.

The invention relates to a Metarhiziumanisopliaevar. anisopliae strain CQMa421 of which the CGMCC (China General Microbiological Culture Collection Center) NO is 4609 and an application thereof in the control of rice leaf roller. The production traits of the strain are good and can grow fast and have high spore-producing capability; and the sporulation rate is up to 6.8%, and the strain has high toxicity. The pesticidal fungal agent produced by using the strain can be used to effectively control the number of the rice leaf rollers.

The invention relates to an application of a Metarhizium anisopliae CQMa421 strain in preparation of insecticide for preventing and controlling Striped flea beetle or plutella xylostella. The strain is capable of infecting Striped flea beetles and plutella xylostella with fatality rates being 60.9% and 70.8% respectively. The Metarhizium anisopliae CQMa421 strain with enhanced toxicity, which is prepared according to a method disclosed by the invention, has a fatality rate reaching 100% and achieves an outstanding prevention and control effect. The insecticide for preventing and controlling Striped flea beetles or plutella xylostella comprises dry conidial powder of Metarhizium anisopliae CQMA421 (44-60%), kieselguhr (30-40%), nano-aluminum trihydrate (0.5-1%), sodium carboxymethylcellulose (3-5%), tween-80 (3-5%) and OP-10 (3-5%). All the components in the Metarhizium anisopliae insecticide are matched effectively to take a synergistic effect. After being mixed with water, the Metarhizium anisopliae insecticide is applied in a spraying way to achieve a field prevention effect of 80% or above for rice planthopper. The Metarhizium anisopliae insecticide has excellent wetting dispersion characteristic, wherein the wetting time is less than 60s; and in addition, the bioactivity during long-term storage can be retained and the storage time is as long as 12 months.

The invention discloses a high-efficiency Metarrhizium anisopliae strain and applications thereof. The strain MaB08 belongs to Eumycota, Mitotic spore fungi, Hyphomycetes, Hyphomycetales, Trichosporon and Metarrhizium, and is preserved in China Center for Type Culture Collection with the preservation date of October 27th, 2008 and the preservation number of CCTCC M 208172. The high-efficiency Metarrhizium anisopliae is an entomopathogenic fungus that has high efficiency for controlling brontispa longissima, and as a living biological pesticide, the high-efficiency Metarrhizium anisopliae has brand new action mechanism that is different from the existing chemical pesticides, has characteristics of non-pollution and non-residue to environment, and can fit for the requirements of organic food production.

The invention discloses entomogenous fungi chafer metarrhizium anisopliae strain CQMa102 acidity trehalase and its manufacturing method. It adopts active chafer metarrhizium anisopliae strain CQMa102 spore to form enzyme in culture medium and purify it. The formed protease is glycoprotein. Its isoelectric point is 4.7. Molecular weight is 170kDa. Trehazolin is its specific inhibition. And its IC50 is 1.3*10-8M. The protein plays important role in trehalose competive in infection insect course. Meanwhile the purified trehalase can be applied to its content qualitative and quantitative detection in industry and other fields.

The invention provides metarhizium rileyi MRJCBT190808 and application thereof to control of spodoptera frugiperda. The metarhizium rileyi MRJCBT190808 is preserved in the China General Microbiological Culture Collection Center on August 21, 2019, the preservation number is CGMCC No.18122, and the preservation address is No.3, No.1 courtyard, Beichen west road, Chaoyang district, Beijing. The metarhizium rileyi MRJCBT190808 is obtained by the processes that wild metarhizium rileyi strains are separated from collected larva bodies of infected spodoptera frugiperda, and cultured on a sacharovi medium, conidia are transferred to a new medium for purification, and then single spore isolation is conducted to obtain the purified metarhizium rileyi MRJCBT190808. The provided metarhizium rileyi MRJCBT190808 is biocontrol fungi capable of being used for control of the spodoptera frugiperda, the characteristics of high pathogenicity to larvae of the spodoptera frugiperda, environmental protection and no pollution, and difficult drug resistance production are achieved, and the metarhizium rileyi MRJCBT190808 can be widely used for biological control of the spodoptera frugiperda.

Alates of the Formosan subterranean termite (FST), Coptotermes formosanus Shiraki, collected after swarming in 2002 died within 48 hrs, and the cadavers were visibly infected with a fungus. Fungi were picked from the cadavers, transferred to media and ultimately isolated to purity. A single fungal isolate, C4-B, taxonomically identified as Metarhizium anisopliae (Metschnikoff), was found to cause rapid mortality of FST alates. This is the first report of a biological control agent for termite alates. Strain C4-B was more lethal to both alates and workers when compared to M. anisopliae strain ESC1, marketed as the termite biocontrol agent BioBlast®. 100% of FST alates exposed to C4-B spores (106 spores / μl) were killed in 3 days. Field studies where C4-B spores were dispersed on grassy lawns resulted in 100% mortality of alates by day 5.

The invention discloses an application of a metarhizium anisopliae CQMa421 strain in the preparation of a medicine for preventing and treating aphids. The medicine for preventing and treating the aphids is prepared from the following raw materials in percentage by weight: 35%-52% of metarhizium anisopliae CQMa421 xerospore powder, 40%-50% of kieselguhr, 1%-2% of nanometer aluminum oxide, 3%-5% of sodium carboxymethylcellulose, 2%-4% of OP-10 and 2%-4% of HEL-40. Black peach aphids and lipaphis erysimi kaltenbach can be infected by the metarhizium anisopliae CQMa421 strain; and the fatality rate on the black peach aphids and the lipaphis erysimi kaltenbach can respectively reach 71.5% and 70.8%. The fatality rate of the metarhizium anisopliae CQMa421 strain prepared by adopting a method for enhancing the toxicity can reach 100% and is improved by 30%; and the effect is very outstanding. Various components of a metarhizium anisopliae preparation effectively cooperate to reach the effect of synergistic interaction. The metarhizium anisopliae preparation disclosed by the invention is sprayed by blending in water; and the field control effect on the black peach aphids and the lipaphis erysimi kaltenbach reaches 80% or above. The preparation has good ultraviolet resistant characteristic and wetting dispersion characteristic; the biological activity in long-term storage is kept; and the storage time can reach 12 months.

The invention relates to Metarhizium rileyi and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Metarhizium rileyi FJMR1 strain disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 18552. The Metarhizium rileyi not only has strong pathogenicity on Spodoptera frugiperda larvae, but also has an ovicidal effect, can effectively control the population quantity of Spodoptera frugiperda, and can solve the technical problems that in the prior art, chemical pesticides are adopted for preventing and treating Spodoptera frugiperda in corn to pollute the environment and easily generate drug resistance.

The invention disclose a strain with high destruxins yield and application thereof. Classification status of the strain MaQ10 is as follows: asexual generation eumycophyta, mitospore eumycophyta, hyphomycetes, moniliales, hyphomycetaceae and metarrhizium; the strain is conserved at China Center for Type Culture Collection, conservation date is October 27, 2008, the conservation number is CCTCC M 208173. The metarrhizium strain has the advantages of high yield of destruxins A and destruxins B, stable strain yield, in particular good stability of destruxins yield before the 10th generation. The strain can provide technical base for increasing the actual yield and reducing production cost of the destruxins.

The invention discloses a metarhizium strain pathogenic to Laos false blister beetles and application of the metarhizium strain. The metarhizium strain is named as metarhizium anisopliae [FJMa200902, which is stored in China general microbiological culture collection center (CGMCC)], and the collection number is CGMCC No.10642. The strain grows fast and has strong spore formation capability, good production traits and high pathogenicity to larvae of the Laos false blister beetles; after the strain is inoculated by a spore suspension liquid of 107 spores / mL, LT50 (lethal time of 50%) of the strain to the larvae of the Laos false blister beetles is 3.6 days, and infection rate reaches 100%. A fungus preparation made from the strain has good prevention and treatment effects on the Laos false blister beetles, population density of the larvae of the Laos false blister beetles can be reduced effectively, and the metarhizium strain is a biocontrol strain which has application value for prevention and treatment of the Laos false blister beetles.

The invention relates to application of a metarhizium anisopliae CQMa421 strain in preparation of a medicament for preventing and treating rice planthoppers. The medicament for preventing and treating the rice planthoppers is prepared from the following components by weight percent: 35-52% of metarhizium anisopliae CQMa421 dry spore powder, 40-50% of diatomite, 1-2% of nanometer silicon, 3-5% of sodium carboxymethylcellulose, 2-4% of O-30 and 2-4% of HEL-40. The rice planthoppers can be infected by the metarhizium anisopliae CQMa421 strain, and the fatality rate can reach 32.6 percent. The metarhizium anisopliae CQMa421 strain prepared by a toxicity increasing method has the fatality rate of 98.7-100%, LT50 is shortened by 57.4-61.4%, and the effect is very prominent. The components of the metarhizium anisopliae preparation are effectively matched to play a synergistic interaction role, and filed efficacy on the rice planthoppers can reach 80% above by spraying the metarhizium anisopliae preparation added with water. The preparation has good ultraviolet resistance and dispersing and wetting properties, the biological activity in long-term storage is kept, and the storage time is up to 12 months.

The invention discloses a culture medium for cultivating high-toxicity Metarhizium anisopliae and a preparation of the Metarhizium anisopliae and application of the preparation, and belong to the technical field of plant protection. The culture medium comprises the following components: wheat bran, rice husks, rice flour, corn flour, polypide lyophilized powder, dipotassium hydrogen phosphate, manganese sulfate, calcium chloride and water. Since the polypide lyophilized powder of Spodoptera frugiperda is introduced into a traditional culture medium, the toxicity of Metarhizium anisopliae NG-Ma040 for Spodoptera frugiperda killing can be improved, and no obvious influences on the sporulation quantity can be caused; meanwhile, through synergistic cooperation among the wheat bran, rice husks,rice flour, corn flour, dipotassium hydrogen phosphate, manganese sulfate, calcium chloride and other components, the toxicity for Spodoptera frugiperda is improved further, and the infection rate and control effect on larvae of Spodoptera frugiperda can be improved; and moreover, the culture medium can be made into the preparation of Metarhizium anisopliae, and the preparation is applied to prevention and control of Spodoptera frugiperda.

The invention discloses an eelworm killing metarhizium and the application thereof. The metarhizium is metarhizium anisopliae var.anisopliae HYN-1-2 which is collected in the China general microbiological culture collection center on 31st October, 2011 and the collection number is CGMCC No.5433. The metarhizium and metarhizium preparations are used for controlling plant meloidogyne; the controlling effect can reach 50-70 percent and is relatively stable; the active constituents of the metarhizium and the metarhizium preparations are good in fixed planting effect in soil, non-toxic and pollution-free in use, high in performance price ratio, convenient in application, and wide in application prospect.

The invention provides recombinant broad-spectrum metarhizium; the recombinant broad-spectrum metarhizium down-regulates the expression of monoamine oxidase or does not express the monoamine oxidase,or the content of tryptamine in a recombinant broad-spectrum metarhizium body is higher than that of tryptamine in wild type broad-spectrum metarhizium; the recombinant broad-spectrum metarhizium is the strain per se, the progenies of the strain, the conidia produced by the strain, the mycelia produced by the strain, or any combination of the progenies, the conidia and the mycelia. A monoamine oxidase gene in the recombinant broad-spectrum metarhizium is knocked out, so that the concentration of tryptamine in a broad-spectrum metarhizium robertsii is significantly increased, the insecticidal efficiency is further obviously increased, and the half lethal time LT50 of the wild-type broad-spectrum metarhizium is shortened to 6.136+ / -0.488 days from 7.33+ / -0.445 days; furthermore, the recombinant broad-spectrum metarhizium is harmless to the environment, good in biological safety and non-toxic to human beings.

The invention relates to a pesticide for preventing and treating grassland spodoptera litura, and relates to a pesticide. The pesticide comprises an attractant, an adjuvant, beer yeast powder, composite vitamin powder, glucose and active metarhizium anisopliae. The pesticide is prepared from the following components in parts by weight: 1-10 parts of an attractant, 2-30 parts of an adjuvant, 1-10 parts of beer yeast powder, 1-15 parts of compound vitamin powder, 1-5 parts of glucose and 40-70 parts of active metarhizium anisopliae. The pesticide has the advantages that the resistance development of the grassland spodoptera litura can be effectively slowed down, the effect of the cyantraniliprole in seed treatment is achieved quickly, the pesticide effect is stable, an excellent prevention and control effect on pests is achieved, and the pest resistance of crops is improved.

The invention discloses an application of a metarhizium anisopliae CQMa421 strain in preparation of agents for controlling ostrinia nubilalis. The metarhizium anisopliae CQMa421 strain can infect the ostrinia nubilalis, the fatality rate can be 66.5 percent; if a metarhizium anisopliae CQMa421 strain prepared by adopting a toxicity enhancing method is adopted, the fatality rate is up to 100%, LT50 is shortened by 60 percent, and the effect is very outstanding. According to the metarhizium anisopliae preparation disclosed by the invention, the various components are effectively matched with one another, a synergistic effect is achieved, and when the metarhizium anisopliae preparation is blended with water to spray, the field efficacy of ostrinia nubilalis is over 80 percent. The preparation has high ultraviolet resistance and wetting and dispersing characteristics, the biological activity of the preparation in long-term storage is maintained, and the storage time can be 12 months.

The invention discloses a microbial preparation for preventing and treating plant diseases, which is characterized by comprising the following microbial preparations in parts by weight: 10-20 parts of bacillus velezensis HAB-2, 5-10 parts of trichoderma harzianum, 1-5 parts of beauveria bassiana and 1-3 parts of metarhizium anisopliae. Wherein the preservation number of the bacillus velezensis HAB-2 is CCTCC (China Center For Type Culture Collection) NO: M 2015070. Correspondingly, the invention further provides a preparation method and application of the microbial preparation. The microbial preparation disclosed by the invention has remarkable prevention and treatment effects on areca-nut anthracnose, guava leaf blight, navel orange anthracnose, longan leaf spot disease, wax apple fruit rot and the like, and can effectively reduce the morbidity.

The invention discloses a Metarhizium taii GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof. The preservation number of the Metarhizium taii GYYA0601 is CGMCC No.2880. The Metarhizium taii GYYA0601 strain is used for producing the antibiotics by fermentation; a fermentation method comprises the steps of strain activation, primary seed preparation and fermentation; and the obtained fermentation product has broad-spectrum antibiotic activity.

The invention relates to a new formula of a culture medium used in culture of metarhizium anisopliae which is mainly used for transforming W-oxides to mildew oxides during the biological fermentation process of sterides, and the new formula of the culture medium belongs to the technical field of fermentation of industrial fungi. The new formula of the culture medium is as follows: each 1000ml of the culture medium contains 2.0%-4.5% of glucose by weight, 0.4%-0.7% of peptone by weight, 0.03%-0.07% of magnesium sulfate by weight, 0.2%-0.6% rapeseed oil cake by weight, 0.1%-0.3% prinsepia utilis royle powder by weight, 0.1%-0.3% cotton seed meal by weight and the balance of water. The metarhizium anisopliae cultured by the culture medium is rapid and uniform in growth, and thalli are less prone to balling generally; and in addition, most of raw materials adopted by the culture medium are waste materials from industrial production, the sources are wide, the yield is great, the metarhizium anisopliae can be supplied all the year round, the production cost is low, and the characteristics of low carbon and circulatory production are simultaneously reflected.

The invention belongs to the technical field of microorganisms, and particularly relates to a solid fermentation culture medium for promoting spore production of metarhizium rileyi as well as a preparation method and application of the solid fermentation culture medium. The solid fermentation culture medium for promoting spore production of metarhizium rileyi is prepared from the following raw materials in parts by mass: 1-3 parts of rice, 1-2 parts of soybeans and 1-2 parts of a supplement, wherein the supplement comprises lactose, glucose, yeast powder, potassium nitrate and peanut oil, andthe mass ratio of lactose to glucose to yeast powder to potassium nitrate to peanut oil is (10-20):(4-8):(16-32):(8-16):(5-10). The spore production quantity of the metarhizium rileyi obtained by adopting the culture medium provided by the invention is greatly improved, the spore production period is short, the spore germination rate reaches 95% or above, and the cost is low.

The invention provides a culture medium used for producing metarhizium anisopliae spore powder and a production method of the high-content spore powder of metarhizium anisopliae. The culture medium comprises a liquid seed culture medium and a solid culture medium which are used for culturing the metarhizium anisopliae, wherein the liquid seed culture medium is composed of the following componentsin percentage by weight: 6% of yam powder, 4% of cockroach powder, 3% of glucose, 0.1% of K2HPO4, 0.05% of MgSO4 and the balance water, and a pH value is 6; and the solid culture medium is composed ofthe following components in percentage by weight: 10-12% of yam herbaceous climber powder, 30-40% of cockroach powder, 20-30% of yam powder, 2-3% of glucose and the balance water, and a pH value is 6. The production method comprises the following steps: rejuvenating the metarhizium anisopliae; conducting liquid seed culture to obtain metarhizium anisopliae mother liquor; and conducting secondaryculture by means of the solid culture medium, and collecting spores. According to the provided production method, no grains are used, a spore yield is high, pollution is not liable to be caused in a fermentation process, and conidia and a substrate are easy to separate.

The invention discloses Metarhizium spore powder produced by an inclined-form fermentation and reaction bin, which adopts straws as major materials and wheat bran and corn meal as accessories while KH2PO4, MgSO4 and CuSO4 are used as fermentation-used culture materials and metarhpium anisophiae is adopted as a strain and then intermittent culture is carried out to the fermentation bin to produce spores. A method includes the steps: the straws are put into a material gas explosion system for carrying out gas explosion; the straws after gas explosion react with the wheat bran and the corn meal in a chelated reaction bin; after the reaction, the materials are put into the fermentation bin and the culture materials and the metarhpium anisophiae are added, and then ventilation and oxygen supply are carried out in the fermentation bin and fermentation lasts for 96 hours; and a packet receiver is used for collecting spores. The spore powder has the advantages that products can effectively control over 130 kinds of plant diseases and insect pests and wide material selection is realized, and more straw wastes are utilized with environmental production, no toxicity, no innocuity, low production cost, high yield, no easy cross contamination and wide application prospects.

The invention discloses metarhizium anisopliae CHMA-005 and application thereof in prevention and control of tea geometrid, and belongs to the technical field of biological prevention and control. Themetarhizium anisopliae CHMA-005 disclosed by invention is simple to culture, rapid in growth on culture medium, large in sporulation quantity and high in spore germination rate, and infection lethaleffect on tea geometrid larvae can reach 80% or above, the metarhizium anisopliae CHMA-005 is relatively safe to natural enemies, capable of replacing part of chemical pesticides of tea geometrid, capable of reducing the dosage of the chemical pesticides and beneficial to maintaining ecological balance.

The invention relates to the technical field of pesticides, in particular to a diatomite-Metarhizium anisopliae pesticide composition and application thereof. According to the invention, the diatomite-Metarhizium anisopliae pesticide composition is prepared by compounding diatomite with a specific zigzag structure and Metarhizium anisopliae; the prepared pesticide composition is relatively good inpesticide effect and relatively wide in insecticidal range, and has relatively good pesticide effects on various pests such as citrus red spiders, citrus scale insects, frankliniella occidentalis, whiteflies, liriomyza sativae, prodenia litura, tea lesser leafhoppers, tea yellow thrips, tea geometrids, apolygus lucorum and grape aphids. Besides, the diatomite-Metarhizium anisopliae wettable powder is further prepared from a specific wetting agent and a dispersing agent, and the prepared diatomite-Metarhizium anisopliae wettable powder is good in wettability and spreadability, high in suspension rate and short in wetting time.

The invention discloses a Metarhizium anisopliae strain with high pathogenicity to larvae of Milionia basalis and application of the Metarhizium anisopliae strain, and belongs to the technical field of biological control. The strain is named Metarhizium anisopliae FJMa201516, and the preservation number is CGMCC No.18123. The strain is high in growth speed and high in spore production capacity, and meanwhile has high pathogenicity to the larvae of the Milionia basalis; after the larvae of the Milionia basalis are inoculated with a 107 spore / mL spore suspension for 15 days, the cumulative deathrate of the larvae reaches 96.7%, and LT50 is 5.01 days; a fungal preparation produced by using the strain has a good control effect on the Milionia basalis, and the population density of the larvaeof the Milionia basalis can be effectively reduced. The strain has potential value in control of the Milionia basalis, and has positive significance in reducing harm caused by the Milionia basalis andreducing the use amount of chemical pesticides.

The invention discloses an insecticidal composition containing metarhizium anisopliae and cyromazine and a preparation method and application of the insecticidal composition.According to the insecticidal composition, metarhizium anisopliae and cyromazine serve as basic active ingredients, and metarhizium anisopliae conidia (piece) and cyromazine (gram) are mixed according to the ratio of (49-1) *2 *1010:(1-49).Wet powder, water dispersible granules and an oil suspending agent can be prepared after auxiliary materials are added to the active ingredients.The insecticidal composition can be used for preventing and treating pests such as scale insects, trialeurodes vaporariorum, thrips, grubs, wireworms, citrus fruit flies, liriomyza sativae, root maggots and Bradysia odoriphaga Yang et Zhang, is especially suitable for preventing and treating diptera pests such as citrus fruit flies, liriomyza sativae, root maggots and bradysia odoriphaga, and has the advantages of being stable in pesticide effect, good in fast-acting property, long in lasting period, safe, environmentally friendly and the like.

The invention provides a seed dressing agent without pesticide residue and a preparation method thereof, and belongs to the field of biological products. The seed dressing agent comprises, by weight,25-30 parts of metarhizium anisopliae, 25-30 parts of beauveria bassiana, 2000-3000 parts of animal protein, 200-300 parts of brown sugar, and 15-20 parts of yeast. Animal protein and brown sugar provide nutrition for beauveria bassiana, metarhizium anisopliae and yeast in the fermentation process. Some toxins can be generated in the fermentation process of metarhizium anisopliae and has high insecticidal activity, and hyphae and spores of metarhizium anisopliae itself also have high bactericidal activity. Toxins, hydrolase, organic acids and other compounds can be generated in the fermentation process of beauveria bassiana to resist insects, and beauvericin, ovosporin and calcium oxalate crystals can also be generated to cause insect poisoning, disruption of metabolism and death. In addition, beauveria bassiana is mainly used for infecting host cells and forming specific sexual organisms after symbiosis with hosts so as to exert a pesticide effect, and beauveria bassiana can also be used for inducing cell apoptosis and the like and can be used for well preventing and treating underground insect pests.

The invention discloses a preparation method of a yield-increasing disease-resistant biological bacterial fertilizer for field crops. The preparation method comprises the following steps: step 1, preparing a first complex microbial inoculant and a second complex microbial inoculant; step 2, screening a plurality of strains from a strain library of verticillium lecanii, beauveria bassiana and metarhizium anisopliae, selecting three fungal strains which have the smallest antagonism with the second complex microbial inoculant obtained in the step 1 from the strains, and carrying out enlarged culture; step 3, preparing a coating agent; step 4, fermenting; step 5, granulating and coating; and step 6, carrying out low-temperature drying in vacuum. The problems that in the prior art, a field biological bacterial fertilizer prepared from plant ash, furfural residues and mushroom dregs does not contain various active microorganisms with different functions, the colonization survival performance of the microorganisms is low, and the disease prevention and insect prevention effects are not achieved can be solved.