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Application of metarhizium anisopliae strain in preparation of medicine for killing aphids

A technology of Metarhizium anisopliae and Metarhizium anisopliae, applied in the direction of insecticide, application, biocide, etc., can solve the problems of short product storage period, poor wet dispersing effect, and large control effect by ultraviolet rays, so as to maintain biological Effect of activity, outstanding effect, good UV resistance and wetting and dispersing properties

Active Publication Date: 2015-05-20
CHONGQING JULIXIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the activity of fungal spores is affected by wetting agents, the dosage of surfactants is often reduced in preparations, resulting in poor wetting and dispersing effects. At the same time, the storage period of the product is still short, and the control effect is greatly affected by ultraviolet rays.

Method used

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  • Application of metarhizium anisopliae strain in preparation of medicine for killing aphids
  • Application of metarhizium anisopliae strain in preparation of medicine for killing aphids
  • Application of metarhizium anisopliae strain in preparation of medicine for killing aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 The virulence, sporulation characteristics and heat resistance characteristics of CQMa421 strain after being inoculated with peach aphid and radish aphid

[0027] Inoculate the CQMa421 strain on a 1 / 4 SDA plate, incubate at 28°C for 15 days, collect mature conidia, disperse them with 0.05% (W / V) Tween-80, and prepare a final concentration of 1×10 7 spores / ml suspension. The green peach aphid larvae reared indoors were transferred into 500ml triangular flasks with cabbage leaves, 30-50 aphids per bottle, sprayed and inoculated with a spray tower (POTTER, BURKARD company), 5 bottles were inoculated, each bottle was inoculated with 0.5 ml. After inoculation, place them in a bioassay room with a room temperature of 28°C and a humidity of 50-70%; select the aphid slugs that are the first to die, and put them in a 28°C incubator for 3-5 days to grow on the surface of the insects. produce fresh spores; collect fresh spores, inoculate radish aphid larvae again, and o...

Embodiment 2

[0034] Example 2 The protective effect of nano-alumina on Metarhizium anisopliae

[0035] According to Table 2, the pure sporopollen of Metarhizium anisopliae CQMa421 and nano-alumina were first mixed, and then 45% diatomaceous earth, 5% CMC and different contents of OP-10 and HEL-40 were added and mixed.

[0036] UV protection: disperse the preparation sample with water to make a concentration of 1×10 7 After spores / mL of the spore suspension, take 100μl of the spore suspension and spread it on a 1 / 4 SDA plate, spread it evenly with glass, and then irradiate it vertically under a 290-310nm ultraviolet lamp (978mW / m2) for 5 hours, then place Cultivate in an incubator at 28°C for 24 hours, measure the germination rate (the method is the same as above), and repeat 3 times. The data were processed and analyzed with SPSS software.

[0037] Storage experiment: Store the preparation sample in a sealed container at 28°C for 2 months, and disperse it with water to make a concentrati...

Embodiment 3

[0042] Example 3 Preparation of wettable powder of Metarhizium anisopliae CQMa421 and its wettability, storage period and control effect.

[0043] According to Table 3, the pure sporopollen of Metarhizium anisopliae CQMa421 and nano-alumina were first mixed, and then diatomaceous earth, CMC, OP-10 and HEL-40 with different contents were added.

[0044] Determination of wettability of wetting and dispersing agent: Take 100mL of standard water and pour it into a 250mL beaker. Place the beaker in a constant temperature water bath at 25°C±1°C, so that the liquid level is flush with the water bath level. When the hard water reaches 25°C±1°C, accurately weigh 5.0g of the sample to be tested and place it on a watch glass. Do not disturb the liquid level excessively. Immediately record the time with a stopwatch when adding the sample until the sample is completely wetted (the fine powder film remaining on the liquid surface can be ignored). Note down the wetting time (accurate to t...

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Abstract

The invention discloses an application of a metarhizium anisopliae CQMa421 strain in the preparation of a medicine for preventing and treating aphids. The medicine for preventing and treating the aphids is prepared from the following raw materials in percentage by weight: 35%-52% of metarhizium anisopliae CQMa421 xerospore powder, 40%-50% of kieselguhr, 1%-2% of nanometer aluminum oxide, 3%-5% of sodium carboxymethylcellulose, 2%-4% of OP-10 and 2%-4% of HEL-40. Black peach aphids and lipaphis erysimi kaltenbach can be infected by the metarhizium anisopliae CQMa421 strain; and the fatality rate on the black peach aphids and the lipaphis erysimi kaltenbach can respectively reach 71.5% and 70.8%. The fatality rate of the metarhizium anisopliae CQMa421 strain prepared by adopting a method for enhancing the toxicity can reach 100% and is improved by 30%; and the effect is very outstanding. Various components of a metarhizium anisopliae preparation effectively cooperate to reach the effect of synergistic interaction. The metarhizium anisopliae preparation disclosed by the invention is sprayed by blending in water; and the field control effect on the black peach aphids and the lipaphis erysimi kaltenbach reaches 80% or above. The preparation has good ultraviolet resistant characteristic and wetting dispersion characteristic; the biological activity in long-term storage is kept; and the storage time can reach 12 months.

Description

technical field [0001] The invention relates to a new application of a strain of Metarhizium anisopliae, in particular to a preparation for killing aphids and an application of the Metarhizium anisopliae, belonging to the field of biological pesticides. Background technique [0002] Cabbage aphid is an important vegetable pest. In addition to direct feeding hazards, it is also the main vector of various plant viruses, and the damage of the latter is often more than that of direct feeding. Among them, the main vegetable aphids in my country, including peach aphid and radish aphid, are broad-feeding pests. The hosts mainly include pears, peaches, plums, plums, cherries and other Rosaceae fruit trees and cabbage, cabbage, radish, mustard greens, brassica, turnips, sweet peppers, Capsicum, spinach and many other crops. Radish aphids can damage cruciferous vegetables such as cabbage, Chinese cabbage, radish, kale, broccoli, and sedge vegetables, and often occur mixed with peach a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04C12N1/14C12N3/00C12R1/645
Inventor 夏玉先彭国雄
Owner CHONGQING JULIXIN BIOENG
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