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Acid trehalosease and preparation process thereof

A technology of trehalase and trehalose, applied in the direction of hydrolytic enzymes, fungi, etc., can solve problems that have not been reported, and achieve the effects of purification, avoidance of interference, and high activity

Inactive Publication Date: 2006-06-28
CHONGQING UNIV +1
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  • Abstract
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Problems solved by technology

However, there is no report on the acid trehalase of entomogenic fungi at home and abroad.

Method used

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  • Acid trehalosease and preparation process thereof
  • Acid trehalosease and preparation process thereof
  • Acid trehalosease and preparation process thereof

Examples

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Embodiment Construction

[0043] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but the present invention is not limited thereto.

[0044] Activation of Spores of Metarhizium anisopliae Strain CQMa102

[0045] The Metarhizium anisopliaevar.acridum CQMa102 strain in this example is isolated and purified from the diseased Bamboo locust anisopliae, isolated and purified by the Genetic Engineering Center of Chongqing University, and preserved by the Chinese Institute of Microorganisms Culture Conservation Center, with a preservation number of CGMCC No.0877, its conidia are stored in 30% glycerin solution and stored in -80℃ ultra-low temperature refrigerator for a long time. The CQMa102 bacterial strain of Metarhizium anisopliae (Metarhizium anisopliae) preserved at -80°C was inoculated on a 1 / 4 SDA plate medium for activation, and the medium used was composed of 10g / l glucose, 2.5g / l peptone, 5.0g / l yeast extract, 20g / l agar, and adjust...

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Abstract

The invention discloses entomogenous fungi chafer metarrhizium anisopliae strain CQMa102 acidity trehalase and its manufacturing method. It adopts active chafer metarrhizium anisopliae strain CQMa102 spore to form enzyme in culture medium and purify it. The formed protease is glycoprotein. Its isoelectric point is 4.7. Molecular weight is 170kDa. Trehazolin is its specific inhibition. And its IC50 is 1.3*10-8M. The protein plays important role in trehalose competive in infection insect course. Meanwhile the purified trehalase can be applied to its content qualitative and quantitative detection in industry and other fields.

Description

technical field [0001] The invention relates to an acid trehalase, in particular to an acid trehalase from an entomogenous fungus and a preparation method thereof. Background technique [0002] It is known that trehalose (α-D-glucoside (1,1)-α-D-glucoside) is a non-reducing disaccharide, which is the main blood sugar in insect hemolymph, and its content is as high as 20-100mM, accounting for the total blood sugar These insects include Locustamigratoria (Elbein, A.D; 1974). Trehalose has an important physiological role in the life activities of insects, which can be summarized as the following four points: (1) as energy storage in insects, (2) as antifreeze protection agent to prevent blood coagulation in insects at low temperatures, (3) as The protein stabilizer prevents the denaturation of hemolymph proteins under conditions such as heat stress, and (4) acts as a feedback regulator of feeding intake to regulate insect feeding. Depletion of trehalose in hemolymph may be le...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12N1/14
Inventor 夏玉先赵华王中康殷幼平彭国雄
Owner CHONGQING UNIV
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