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Metarhizium rileyi MRJCBT190808 and application thereof to control of spodoptera frugiperda

A technology of MRJCBT190808, Spodoptera frugiperda, which is applied in the field of microorganisms, can solve the problems of ecological environment impact, human and animal safety hazards, pesticide residues, etc., and achieve the effects of high spore germination rate, rapid growth, and large spore production

Active Publication Date: 2020-01-10
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, chemical control methods are mainly used for the control of Spodoptera frugiperda at present, and the insect has developed strong resistance to many pesticides
In addition, problems such as pesticide residues caused by the use of chemical control methods have hidden dangers to human and animal safety and risks to the ecological environment.

Method used

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  • Metarhizium rileyi MRJCBT190808 and application thereof to control of spodoptera frugiperda

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. Isolation and identification of Metarhizium anisopliae MRJCBT190808

[0018] 1.1 Materials

[0019] 1.1.1 Tested strains

[0020] The strain was collected from the diseased larvae of Spodoptera frugiperda in Baotaying Village, Jiangchuan County, Yuxi City, Yunnan Province on August 8, 2019.

[0021] 1.1.2 Isolation and cultivation of strains

[0022] Put the collected diseased Spodoptera frugiperda worms into a 5mL sterile EP tube and bring them back to the laboratory, and use the streak method to place them on PDA medium (200g potato, 20g glucose, 20g agar, 1000mL distilled water, natural pH) , cultivated in a light incubator at (25±1)°C, L / / D=16h / / 8h, humidity 80%. After 7 days of culture, the conidia were transferred to a new medium for purification. Purified strains were stored in a 4°C refrigerator using SDAY slant medium.

[0023] 1.1.3 Colony morphology observation

[0024] Inoculate the isolated and purified fungi on PDA (potato 200g, glucose 20...

Embodiment 2

[0027] Example 2. Pathogenicity determination of Metarhizium anisopliae MRJCBT190808 to Spodoptera frugiperda

[0028] 1 material

[0029] 1.1.1 Tested insects

[0030] Spodoptera frugiperda was collected in the cornfield planting area (Banqiao Town, Luliang County, Qujing City, Yunnan Province) and was continuously raised indoors for 3 generations. Carried out in an intelligent artificial incubator (RG-300) under the condition of cycle L:D=16:8. Spodoptera frugiperda was reared artificially with fresh corn kernels and leaves.

[0031] 1.1 Preparation of spore suspension

[0032] The isolated bacterial strain is inoculated on the PDA medium, and after it produces a large amount of conidia (about 20d), the conidia on the surface of the medium are scraped with a sterile scalpel and placed in 0.02% sterile Tween-80 , formulated as 2.96×10 3 –2.96×10 8 individual / mL spore suspension.

[0033] 1.1.2 Dipping method

[0034] Select healthy 3rd instar larvae of Spodoptera frug...

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Abstract

The invention provides metarhizium rileyi MRJCBT190808 and application thereof to control of spodoptera frugiperda. The metarhizium rileyi MRJCBT190808 is preserved in the China General Microbiological Culture Collection Center on August 21, 2019, the preservation number is CGMCC No.18122, and the preservation address is No.3, No.1 courtyard, Beichen west road, Chaoyang district, Beijing. The metarhizium rileyi MRJCBT190808 is obtained by the processes that wild metarhizium rileyi strains are separated from collected larva bodies of infected spodoptera frugiperda, and cultured on a sacharovi medium, conidia are transferred to a new medium for purification, and then single spore isolation is conducted to obtain the purified metarhizium rileyi MRJCBT190808. The provided metarhizium rileyi MRJCBT190808 is biocontrol fungi capable of being used for control of the spodoptera frugiperda, the characteristics of high pathogenicity to larvae of the spodoptera frugiperda, environmental protection and no pollution, and difficult drug resistance production are achieved, and the metarhizium rileyi MRJCBT190808 can be widely used for biological control of the spodoptera frugiperda.

Description

technical field [0001] The application belongs to the technical field of microorganisms, and in particular relates to a Metarhizium anisopliae MRJCBT190808 and its application in the control of Spodoptera frugiperda. Background technique [0002] Spodoptera frugiperda (J.E.Smith)], also known as fall armyworm, belongs to Lepidoptera, Noctuidae, is a kind of omnivorous pest native to tropical and subtropical regions of America, especially harmful to corn serious. Since it first invaded Jiangcheng County, Pu'er City, Yunnan Province in January 2019, it has spread to more than 1,000 counties in 19 provinces in my country, seriously endangering corn and other crops, and has become an important factor seriously affecting the development of the corn industry. [0003] In the prior art, chemical control methods are mainly used for the control of Spodoptera frugiperda at present, and the insect has developed strong resistance to many pesticides. In addition, problems such as pesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P7/04C12R1/645
CPCC12N1/145C12R2001/645
Inventor 陈斌郑亚强肖关丽杜广祖和淑琪桂富荣李正跃
Owner YUNNAN AGRICULTURAL UNIVERSITY
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